PMID- 33269744
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210703
IS  - 1673-5374 (Print)
IS  - 1876-7958 (Electronic)
IS  - 1673-5374 (Linking)
VI  - 16
IP  - 6
DP  - 2021 Jun
TI  - Electroacupuncture improves learning and memory functions in a rat cerebral 
      ischemia/reperfusion injury model through PI3K/Akt signaling pathway activation.
PG  - 1011-1016
LID - 10.4103/1673-5374.300454 [doi]
AB  - Electroacupuncture has been widely used to treat cognitive impairment after 
      cerebral ischemia, but the underlying mechanism has not yet been fully 
      elucidated. Studies have shown that autophagy plays an important role in the 
      formation and development of cognitive impairment, and the phosphoinositide 
      3-kinase (PI3K)/Akt signaling pathway plays an important role in autophagy 
      regulation. To investigate the role played by the PI3K/Akt signaling pathway in 
      the electroacupuncture treatment of cerebral ischemia/reperfusion rat models, we 
      first established a rat model of cerebral ischemia/reperfusion through the 
      occlusion of the middle cerebral artery using the suture method. Starting at 2 
      hours after modeling, electroacupuncture was delivered at the Shenting (GV24) and 
      Baihui (GV20) acupoints, with a dilatational wave (1-20 Hz frequency, 2 mA 
      intensity, 6 V peak voltage), for 30 minutes/day over 8 consecutive days. Our 
      results showed that electroacupuncture reduced the infarct volume in a rat model 
      of cerebral ischemia/reperfusion injury, increased the mRNA expression levels of 
      the PI3K/Akt signaling pathway-related factors Beclin-1, mammalian target of 
      rapamycin (mTOR), and PI3K, increased the protein expression levels of 
      phosphorylated Akt, Beclin-1, PI3K, and mTOR in the ischemic cerebral cortex, and 
      simultaneously reduced p53 mRNA and protein expression levels. In the Morris 
      water maze test, the latency to find the hidden platform was significantly 
      shortened among rats subjected to electroacupuncture stimulation compared with 
      rats without electroacupuncture stimulation. In the spatial probe test, the 
      number of times that a rat crossed the target quadrant was increased in rats 
      subjected to electroacupuncture stimulation compared with rats without 
      electroacupuncture stimulation. Electroacupuncture stimulation applied to the 
      Shenting (GV24) and Baihui (GV20) acupoints activated the PI3K/Akt signaling 
      pathway and improved rat learning and memory impairment. This study was approved 
      by the Animal Ethics Committee of the First Affiliated Hospital of Henan 
      University of Traditional Chinese Medicine, China (approval No. 8150150901) on 
      March 10, 2016.
FAU - Wang, Hui-Ling
AU  - Wang HL
AD  - Rehabilitation Center, the First Affiliated Hospital of Henan University of 
      Traditional Chinese Medicine, Zhengzhou, Henan Province, China.
FAU - Liu, Fei-Lai
AU  - Liu FL
AD  - Rehabilitation Center, the First Affiliated Hospital of Henan University of 
      Traditional Chinese Medicine, Zhengzhou, Henan Province, China.
FAU - Li, Rui-Qing
AU  - Li RQ
AD  - Rehabilitation Center, the First Affiliated Hospital of Henan University of 
      Traditional Chinese Medicine, Zhengzhou, Henan Province, China.
FAU - Wan, Ming-Yue
AU  - Wan MY
AD  - Department of Rehabilitation Medicine, Fujian University of Chinese Medicine, 
      Fuzhou, Fujian Province, China.
FAU - Li, Jie-Ying
AU  - Li JY
AD  - Department of Rehabilitation Medicine, Henan University of Chinese Medicine, 
      Zhengzhou, Henan Province, China.
FAU - Shi, Jing
AU  - Shi J
AD  - Department of Rehabilitation Medicine, Henan University of Chinese Medicine, 
      Zhengzhou, Henan Province, China.
FAU - Wu, Ming-Li
AU  - Wu ML
AD  - Rehabilitation Center, the First Affiliated Hospital of Henan University of 
      Traditional Chinese Medicine, Zhengzhou, Henan Province, China.
FAU - Chen, Jun-Hua
AU  - Chen JH
AD  - Rehabilitation Center, the First Affiliated Hospital of Henan University of 
      Traditional Chinese Medicine, Zhengzhou, Henan Province, China.
FAU - Sun, Wei-Juan
AU  - Sun WJ
AD  - Rehabilitation Center, the First Affiliated Hospital of Henan University of 
      Traditional Chinese Medicine, Zhengzhou, Henan Province, China.
FAU - Feng, Hong-Xia
AU  - Feng HX
AD  - Rehabilitation Center, the First Affiliated Hospital of Henan University of 
      Traditional Chinese Medicine, Zhengzhou, Henan Province, China.
FAU - Zhao, Wei
AU  - Zhao W
AD  - Rehabilitation Center, the First Affiliated Hospital of Henan University of 
      Traditional Chinese Medicine, Zhengzhou, Henan Province, China.
FAU - Huang, Jin
AU  - Huang J
AD  - Department of Rehabilitation Medicine, Henan University of Chinese Medicine, 
      Zhengzhou, Henan Province, China.
FAU - Liu, Ren-Chao
AU  - Liu RC
AD  - Department of Rehabilitation Medicine, Henan University of Chinese Medicine, 
      Zhengzhou, Henan Province, China.
FAU - Hao, Wen-Xue
AU  - Hao WX
AD  - Department of Rehabilitation Medicine, Fujian University of Chinese Medicine, 
      Fuzhou, Fujian Province, China.
FAU - Feng, Xiao-Dong
AU  - Feng XD
AD  - Rehabilitation Center, the First Affiliated Hospital of Henan University of 
      Traditional Chinese Medicine, Zhengzhou, Henan Province, China.
LA  - eng
PT  - Journal Article
PL  - India
TA  - Neural Regen Res
JT  - Neural regeneration research
JID - 101316351
PMC - PMC8224106
OTO - NOTNLM
OT  - acupuncture
OT  - brain
OT  - central nervous system
OT  - factor
OT  - neurological function
OT  - pathways
OT  - protein
OT  - stroke
COIS- None
EDAT- 2020/12/04 06:00
MHDA- 2020/12/04 06:01
PMCR- 2020/11/27
CRDT- 2020/12/03 08:35
PHST- 2020/12/03 08:35 [entrez]
PHST- 2020/12/04 06:00 [pubmed]
PHST- 2020/12/04 06:01 [medline]
PHST- 2020/11/27 00:00 [pmc-release]
AID - NeuralRegenRes_2021_16_6_1011_300454 [pii]
AID - NRR-16-1011 [pii]
AID - 10.4103/1673-5374.300454 [doi]
PST - ppublish
SO  - Neural Regen Res. 2021 Jun;16(6):1011-1016. doi: 10.4103/1673-5374.300454.