PMID- 33269956
OWN - NLM
STAT- MEDLINE
DCOM- 20210818
LR  - 20210818
IS  - 1532-2297 (Electronic)
IS  - 1082-6068 (Linking)
VI  - 51
IP  - 7
DP  - 2021
TI  - Isolation, identification and optimization of enhanced production of laccase from 
      Galactomyces geotrichum under solid-state fermentation.
PG  - 659-668
LID - 10.1080/10826068.2020.1848867 [doi]
AB  - Laccases are a group of oxidases that catalyze the oxidation of a wide range of 
      electron rich substrates like phenolic compounds, lignin and aromatic amines. 
      They are of interest because of their potential to be used in environmental and 
      industrial applications. In this research, potent laccase producer fungi were 
      screened and isolated from olive mill wastewater (OMW). One of the 23 isolated 
      fungi was identified as Galactomyces geotrichum based on 18S rDNA sequence 
      analysis that detected good laccase activity. Produced laccase had a molecular 
      weight of 55 kDa that was confirmed by zymogram analysis. This is the first 
      report about the optimization of laccase Production by G. geotrichum under 
      solid-state fermentation. The optimization was made by the Taguchi design of 
      experiments (DOE) methodology. An orthogonal array (L25) was designed using 
      Minitab 19 software to study four effective process factors in five levels for 
      laccase production. The optimum condition derived was; moisture content (80%), 
      fermentation time (14 day), CuSO(4)⋅5H(2)O as the inducer (300 muM), glucose as a 
      co-substrate (5 g/L). Maximum laccase activity of 52.86 (U/g of dry substrate) 
      was obtained using optimum fermentation condition. This study aimed to better 
      understand the laccase producing microorganisms in OMW and take them to OMW 
      treatment that is rich in phenolic compounds.
FAU - Pourkhanali, Khadijeh
AU  - Pourkhanali K
AUID- ORCID: 0000-0002-2025-8821
AD  - Department of Chemical Engineering, Faculty of Engineering, University of Guilan, 
      Rasht, Iran.
FAU - Khayati, Gholam
AU  - Khayati G
AD  - Department of Chemical Engineering, Faculty of Engineering, University of Guilan, 
      Rasht, Iran.
FAU - Mizani, Farhang
AU  - Mizani F
AD  - Department of Chemistry, Payame Noor University, Tehran, Iran.
FAU - Raouf, Fereshteh
AU  - Raouf F
AD  - Department of Chemical Engineering, Faculty of Engineering, University of Guilan, 
      Rasht, Iran.
LA  - eng
PT  - Journal Article
PT  - Video-Audio Media
DEP - 20201203
PL  - England
TA  - Prep Biochem Biotechnol
JT  - Preparative biochemistry & biotechnology
JID - 9607037
RN  - 0 (Fungal Proteins)
RN  - EC 1.10.3.2 (Laccase)
RN  - Geotrichum candidum
SB  - IM
MH  - *Fungal Proteins/biosynthesis/chemistry/isolation & purification
MH  - Geotrichum/*growth & development
MH  - *Laccase/biosynthesis/chemistry/isolation & purification
OTO - NOTNLM
OT  - Galactomyces geotrichum
OT  - isolation
OT  - laccase
OT  - olive mill wastewater
OT  - optimization
OT  - solid-state fermentation
EDAT- 2020/12/04 06:00
MHDA- 2021/08/19 06:00
CRDT- 2020/12/03 12:08
PHST- 2020/12/04 06:00 [pubmed]
PHST- 2021/08/19 06:00 [medline]
PHST- 2020/12/03 12:08 [entrez]
AID - 10.1080/10826068.2020.1848867 [doi]
PST - ppublish
SO  - Prep Biochem Biotechnol. 2021;51(7):659-668. doi: 10.1080/10826068.2020.1848867. 
      Epub 2020 Dec 3.