PMID- 33271950
OWN - NLM
STAT- MEDLINE
DCOM- 20210309
LR  - 20210415
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 21
IP  - 23
DP  - 2020 Dec 1
TI  - The Impact of Human Lipoaspirate and Adipose Tissue-Derived Stem Cells Contact 
      Culture on Breast Cancer Cells: Implications in Breast Reconstruction.
LID - 10.3390/ijms21239171 [doi]
LID - 9171
AB  - BACKGROUND: Autologous fat transfer in the form of lipoaspirates for the 
      reconstruction of the breast after breast cancer surgery is a commonly used 
      procedure in plastic surgery. However, concerns regarding the oncologic risk of 
      nutrient-rich fat tissue are widely debated. Previous studies have primarily 
      focused on studying the interaction between adipose-derived stem cells (ASCs) and 
      breast cancer cells. METHODS: In this study, we performed a comprehensive 
      analysis of the paracrine- and contact-based interactions between lipoaspirates, 
      ASCs and breast cancer cell lines. An inverted flask culture method was used to 
      study the contact-based interaction between lipoaspirates and breast cancer 
      cells, while GFP-expressing breast cancer cell lines were generated to study the 
      cell-cell contact interaction with ASCs. Three different human breast cancer cell 
      lines, MCF-7, MDA-MB-231 and BT-474, were studied. We analyzed the impact of 
      these interactions on the proliferation, cell cycle and epithelial-to-mesenchymal 
      (EMT) transition of the breast cancer cells. RESULTS: Our results revealed that 
      both lipoaspirates and ASCs do not increase the proliferation rate of the breast 
      cancer cells either through paracrine- or contact-dependent interactions. We 
      observed that lipoaspirates selectively inhibit the proliferation of MCF-7 cells 
      in contact co-culture, driven by the retinoblastoma (Rb) protein activity 
      mediating cell cycle arrest. Additionally, ASCs inhibited MDA-MB-231 breast 
      cancer cell proliferation in cell-cell contact-dependent interactions. 
      Quantitative real-time PCR revealed no significant increase in the EMT-related 
      genes in breast cancer cells upon co-culture with ASCs. CONCLUSION: In 
      conclusion, this study provides evidence of the non-oncogenic character of 
      lipoaspirates and supports the safety of clinical fat grafting in breast 
      reconstruction after oncological surgical procedures. In vivo studies in 
      appropriate animal models and long-term post-operative clinical data from 
      patients are essential to reach the final safety recommendations.
FAU - Ejaz, Asim
AU  - Ejaz A
AD  - Department of Plastic Surgery, University of Pittsburgh, Pittsburgh, PA 15261, 
      USA.
FAU - Yang, Katherine S
AU  - Yang KS
AD  - Department of Plastic Surgery, University of Pittsburgh, Pittsburgh, PA 15261, 
      USA.
FAU - Venkatesh, Kaushik P
AU  - Venkatesh KP
AD  - Department of Plastic Surgery, University of Pittsburgh, Pittsburgh, PA 15261, 
      USA.
FAU - Chinnapaka, Somaiah
AU  - Chinnapaka S
AD  - Department of Plastic Surgery, University of Pittsburgh, Pittsburgh, PA 15261, 
      USA.
FAU - Kokai, Lauren E
AU  - Kokai LE
AD  - Department of Plastic Surgery, University of Pittsburgh, Pittsburgh, PA 15261, 
      USA.
FAU - Rubin, J Peter
AU  - Rubin JP
AD  - Department of Plastic Surgery, University of Pittsburgh, Pittsburgh, PA 15261, 
      USA.
LA  - eng
GR  - R01 CA114246/CA/NCI NIH HHS/United States
GR  - R01-CA114246-08A1/NH/NIH HHS/United States
PT  - Journal Article
DEP - 20201201
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Biomarkers)
RN  - 0 (Culture Media, Conditioned)
SB  - IM
MH  - Adipose Tissue/*cytology
MH  - Biomarkers
MH  - Breast Neoplasms/*metabolism/pathology
MH  - *Cell Communication
MH  - Cell Differentiation
MH  - Cell Proliferation
MH  - Coculture Techniques
MH  - Culture Media, Conditioned
MH  - Female
MH  - Humans
MH  - Immunophenotyping
MH  - Lipectomy
MH  - Mammaplasty
MH  - Primary Cell Culture
MH  - Stem Cells/*metabolism
PMC - PMC7731376
OTO - NOTNLM
OT  - adipose-derived stem cells
OT  - breast cancer cells
OT  - cell cycle and proliferation
OT  - lipoaspirates
COIS- The authors indicated no potential conflict of interest.
EDAT- 2020/12/05 06:00
MHDA- 2021/03/10 06:00
PMCR- 2020/12/01
CRDT- 2020/12/04 01:01
PHST- 2020/08/27 00:00 [received]
PHST- 2020/11/19 00:00 [revised]
PHST- 2020/11/27 00:00 [accepted]
PHST- 2020/12/04 01:01 [entrez]
PHST- 2020/12/05 06:00 [pubmed]
PHST- 2021/03/10 06:00 [medline]
PHST- 2020/12/01 00:00 [pmc-release]
AID - ijms21239171 [pii]
AID - ijms-21-09171 [pii]
AID - 10.3390/ijms21239171 [doi]
PST - epublish
SO  - Int J Mol Sci. 2020 Dec 1;21(23):9171. doi: 10.3390/ijms21239171.