PMID- 33275232
OWN - NLM
STAT- MEDLINE
DCOM- 20210629
LR  - 20211204
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 24
IP  - 22
DP  - 2020 Nov
TI  - MiR-20a suppresses proliferation and facilitates apoptosis of breast cancer cells 
      via the MTOR signaling pathway.
PG  - 11650-11657
LID - 23809 [pii]
LID - 10.26355/eurrev_202011_23809 [doi]
AB  - OBJECTIVE: The paper aimed to explore the role of micro ribonucleic acid 
      (miR)-20a in regulating the proliferation and apoptosis of breast cancer cells. 
      MATERIALS AND METHODS: The expression of miR-20a in breast cancer cells was 
      analyzed via quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) assay. 
      Cell Counting Kit-8 (CCK-8) assay, colony formation assay, and flow cytometry 
      were employed to analyze the proliferation and apoptosis of cells. Thereafter, 
      the target proteins of miR-20a were predicted using TargetScan, a website for 
      miRNA target gene prediction, and the interaction between miR-20a and the target 
      genes was detected through the Luciferase reporter gene assay, qRT-PCR assay, and 
      Western blotting. Finally, the miR-20a inhibitor and target gene expression 
      plasmids were co-transfected for rescue experiment to study whether the target 
      genes participate in the inhibitory effect of miR-20a on the proliferation of 
      breast cancer cells. RESULTS: It was found that the expression of miR-20a was 
      upregulated in breast cancer cell lines. Silencing miR-20a expression inhibited 
      the proliferation and promoted the apoptosis of breast cancer cell. Besides, it 
      was demonstrated that late endosomal/lysosomal adaptor, mitogen-activated protein 
      kinase (MAPK), and mammalian target of rapamycin (mTOR) activator 3 (LAMTOR3) 
      were a direct target of miR-20a. The knockdown of LAMTOR3 expression repressed 
      the influence of miR-20a on the proliferation of breast cancer cells. 
      CONCLUSIONS: MiR-20a targets LAMTOR3 gene to regulate the mTOR signaling pathway, 
      thereby suppressing the proliferation and facilitating the apoptosis of breast 
      cancer cells. It suggests that miR-20a exerts a carcinogenic effect in breast 
      cancer, which may be a potential target for the treatment of breast cancer.
FAU - Shi, K-Y
AU  - Shi KY
AD  - Department of Ultrasound, The Cancer Hospital of the University of Chinese 
      Academy of Sciences (Zhejiang Cancer Hospital), Zhejiang, China. 
      wanglj@zjcc.org.cn.
FAU - Fan, L-Y
AU  - Fan LY
FAU - Xu, D
AU  - Xu D
FAU - Ren, L-P
AU  - Ren LP
FAU - Wang, L-P
AU  - Wang LP
FAU - Chen, L-Y
AU  - Chen LY
FAU - Wang, L-J
AU  - Wang LJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (LAMTOR3 protein, human)
RN  - 0 (MIRN20a microRNA, human)
RN  - 0 (MicroRNAs)
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing/genetics/metabolism
MH  - Apoptosis
MH  - Breast Neoplasms/*metabolism/pathology
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Female
MH  - Humans
MH  - MicroRNAs/genetics/*metabolism
MH  - Signal Transduction
MH  - TOR Serine-Threonine Kinases/genetics/*metabolism
EDAT- 2020/12/05 06:00
MHDA- 2021/06/30 06:00
CRDT- 2020/12/04 12:08
PHST- 2020/12/04 12:08 [entrez]
PHST- 2020/12/05 06:00 [pubmed]
PHST- 2021/06/30 06:00 [medline]
AID - 23809 [pii]
AID - 10.26355/eurrev_202011_23809 [doi]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2020 Nov;24(22):11650-11657. doi: 
      10.26355/eurrev_202011_23809.