PMID- 33276196
OWN - NLM
STAT- MEDLINE
DCOM- 20210607
LR  - 20240226
IS  - 1878-1705 (Electronic)
IS  - 1567-5769 (Linking)
VI  - 90
DP  - 2021 Jan
TI  - Apolipoprotein A4 regulates the immune response in carbon tetrachloride-induced 
      chronic liver injury in mice.
PG  - 107222
LID - S1567-5769(20)33689-4 [pii]
LID - 10.1016/j.intimp.2020.107222 [doi]
AB  - This article explores the role of ApoA4 in a CCl(4)-induced chronic liver injury 
      (CLI) mouse model. C57BL/6J mice (WT) and ApoA4 knock-out (KO) mice were divided 
      into CCl(4) CLI (WT-CCl(4) and KO-CCl(4)) and olive oil solvent control groups 
      (WT-Veh and KO-Veh). Some of the KO-CCl(4) mice were additionally treated with 
      recombinant mouse ApoA4 and primary mouse T lymphocyte injections. After 6 weeks, 
      histological analyses, biochemical and superoxide dismutase (SOD) and 
      malondialdehyde (MDA) assays, flow cytometry of immune cells and qRT-PCR analyses 
      were performed. KO mice after treatment with CCl(4) showed reduced hepatic SOD 
      and enhanced serum MDA activities leading to worsening liver injury and fibrosis 
      compared with WT-CCl(4), accompanied by enhanced hepatic alpha smooth muscle 
      actin (alpha-SMA), tissue inhibitor of metalloproteinases-1 (TIMP-1) and collagen 
      type I alpha 1 chain (COL1A1) transcriptions, elevated macrophage M1 levels, 
      enhanced tumor necrosis factor-alpha (TNF-alpha), Interleukin 6 (IL-6) and C-C Motif 
      Chemokine Ligand 5 (CCL5), but reduced Interleukin 10 (IL-10), monocyte 
      chemotactic protein 1 (MCP-1), C-C Motif Chemokine Receptor 2 (CCR2), C-X3-C 
      Motif Chemokine Receptor 1 (CX3CR1) and C-X-C Motif Chemokine Ligand 9 (CXCL9) 
      transcription, as well as reduced CD3+, CD4+ and CD8+ T cell percentages in 
      hepatic tissue, blood cells and spleen. In addition, CD11b+CD115+, 
      CD11b+/Ly6C(high), CD11b+/LyC6(-) and CD11b+/Ly6C(int) cells were enhanced, which 
      partly reversed by ApoA4 protein and T cell injections. In conclusion, we propose 
      that ApoA4 might be involved in liver protection via inhibiting fibrotic 
      mediators and inflammatory cytokines, suppression of pro-inflammatory hepatic M1 
      cell invasion and regulation of CD8+ T and CD4+ T lymphocytes.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Wang, Yinan
AU  - Wang Y
AD  - Bio-evidence Science Academy (BSA), Xi'an Jiaotong University (XJTU), No. 205 
      Zhuque Street, Xi'an 710061, China; Key laboratory of Ministry of Public Health 
      for Forensic Sciences, No. 205 Zhuque Street, Xi'an 710061, China.
FAU - Yang, Ziyu
AU  - Yang Z
AD  - Bio-evidence Science Academy (BSA), Xi'an Jiaotong University (XJTU), No. 205 
      Zhuque Street, Xi'an 710061, China; Key laboratory of Ministry of Public Health 
      for Forensic Sciences, No. 205 Zhuque Street, Xi'an 710061, China.
FAU - Wei, Yang
AU  - Wei Y
AD  - National & Local Joint Engineering Research Center of Biodiagnosis and 
      Biotherapy, Precision Medical Institute, Institute of Digestive Diseases. The 
      Second Affiliated Hospital, Xi'an Jiaotong University, No. 157 West 5th Road, 
      Xi'an 710004, China.
FAU - Li, Xiaoming
AU  - Li X
AD  - National & Local Joint Engineering Research Center of Biodiagnosis and 
      Biotherapy, Precision Medical Institute, Institute of Digestive Diseases. The 
      Second Affiliated Hospital, Xi'an Jiaotong University, No. 157 West 5th Road, 
      Xi'an 710004, China. Electronic address: lixiaoming022916@163.com.
FAU - Li, Shengbin
AU  - Li S
AD  - Bio-evidence Science Academy (BSA), Xi'an Jiaotong University (XJTU), No. 205 
      Zhuque Street, Xi'an 710061, China; Key laboratory of Ministry of Public Health 
      for Forensic Sciences, No. 205 Zhuque Street, Xi'an 710061, China. Electronic 
      address: shengbin_li19@163.com.
LA  - eng
PT  - Journal Article
DEP - 20201201
PL  - Netherlands
TA  - Int Immunopharmacol
JT  - International immunopharmacology
JID - 100965259
RN  - 0 (Apolipoproteins A)
RN  - 0 (Cytokines)
RN  - 0 (Inflammation Mediators)
RN  - 0 (apolipoprotein A-IV)
RN  - CL2T97X0V0 (Carbon Tetrachloride)
SB  - IM
MH  - Adoptive Transfer
MH  - Animals
MH  - Apolipoproteins A/genetics/*metabolism
MH  - CD4-Positive T-Lymphocytes/immunology/metabolism
MH  - CD8-Positive T-Lymphocytes/immunology/metabolism
MH  - Carbon Tetrachloride
MH  - Chemical and Drug Induced Liver Injury, Chronic/immunology/*metabolism/pathology
MH  - Chemotaxis, Leukocyte
MH  - Cytokines/genetics/*metabolism
MH  - Gene Expression Regulation
MH  - Inflammation Mediators/*metabolism
MH  - Liver/immunology/metabolism/pathology
MH  - Liver Cirrhosis/chemically induced/immunology/*metabolism/pathology
MH  - Macrophages/immunology/metabolism
MH  - Male
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Oxidative Stress
MH  - Phenotype
MH  - Signal Transduction
MH  - Mice
OTO - NOTNLM
OT  - Anti-fibrosis
OT  - Anti-inflammatory effect
OT  - Anti-oxidative effect
OT  - ApoA4
OT  - CCl(4)-induced chronic liver injury (CLI) mouse model
OT  - Cytokines
EDAT- 2020/12/05 06:00
MHDA- 2021/06/08 06:00
CRDT- 2020/12/04 20:09
PHST- 2020/06/24 00:00 [received]
PHST- 2020/11/02 00:00 [revised]
PHST- 2020/11/16 00:00 [accepted]
PHST- 2020/12/05 06:00 [pubmed]
PHST- 2021/06/08 06:00 [medline]
PHST- 2020/12/04 20:09 [entrez]
AID - S1567-5769(20)33689-4 [pii]
AID - 10.1016/j.intimp.2020.107222 [doi]
PST - ppublish
SO  - Int Immunopharmacol. 2021 Jan;90:107222. doi: 10.1016/j.intimp.2020.107222. Epub 
      2020 Dec 1.