PMID- 33277599
OWN - NLM
STAT- MEDLINE
DCOM- 20210414
LR  - 20240330
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 10
IP  - 1
DP  - 2020 Dec 4
TI  - Nanohybrid-based immunosensor prepared for Helicobacter pylori BabA antigen 
      detection through immobilized antibody assembly with @ Pd(nano)/rGO/PEDOT sensing 
      platform.
PG  - 21217
LID - 10.1038/s41598-020-78068-w [doi]
LID - 21217
AB  - The gastric colonization of human hosts by Helicobacter pylori (H. pylori) 
      increases the risk of developing gastritis, ulcers and gastric cancer. To detect 
      H. pylori, a nanohybrid-based BabA immunosensor is developed herein. BabA is an 
      outer membrane protein and one of the major virulence factors of H. pylori. To 
      design the immunosensor, an Au electrode is loaded with palladium 
      nanoparticles (Pd(nano)) by electrodeposition to generate reduced graphene oxide 
      (rGO)/poly(3,4-ethylenedioxythiophene) (PEDOT). The immobilization of these 
      nanostructured materials imparts a large surface area and electroconductivity to 
      bio-immune-sensing molecules (here, the BabA antigen and antibodies). After 
      optimization, the fabricated immunosensor has the ability to detect antigens (H. 
      pylori) in a linear range from 0.2 to 20 ng/mL with a low LOD (0.2 ng/mL). The 
      developed immunosensor is highly specific, sensitive and reproducible. 
      Additionally, in silico methods were employed to better understand the hybrid 
      nanomaterials of the fabricated Pd(nano)/rGO/PEDOT/Au electrode. Simulations 
      performed by molecular docking, and Metropolis Monte Carlo adsorption studies 
      were conducted. The results revealed that the hybrid nanomaterials exhibit a 
      stable antigen-antibody complex of BabA, yielding the lowest binding energy in 
      relation to the electrode materials, emphasizing the functionality of the 
      constructed electrodes in the electrochemical immunosensor.
FAU - Gupta, Shaivya
AU  - Gupta S
AD  - Amity Institute of Nanotechnology, Amity University, Noida, Uttar Pradesh, 
      201303, India.
FAU - Jain, Utkarsh
AU  - Jain U
AD  - Amity Institute of Nanotechnology, Amity University, Noida, Uttar Pradesh, 
      201303, India.
FAU - Murti, Bayu Tri
AU  - Murti BT
AD  - Department of Chemistry, Durban University of Technology, Durban, 4000, South 
      Africa.
AD  - Semarang College of Pharmaceutical Sciences, Jl. Letnand Jendral Sarwo Edi 
      Wibowo, Semarang City, 50192, Indonesia.
FAU - Putri, Athika Darumas
AU  - Putri AD
AD  - Department of Chemistry, Durban University of Technology, Durban, 4000, South 
      Africa.
AD  - Semarang College of Pharmaceutical Sciences, Jl. Letnand Jendral Sarwo Edi 
      Wibowo, Semarang City, 50192, Indonesia.
FAU - Tiwari, Ashutosh
AU  - Tiwari A
AD  - Institute of Advanced Materials, IAAM, Gammalkilsvagen 18, 590 53, Ulrika, 
      Sweden.
AD  - VBRI, 7/16 Kalkaji Extn., New Delhi, 110 019, India.
FAU - Chauhan, Nidhi
AU  - Chauhan N
AD  - Amity Institute of Nanotechnology, Amity University, Noida, Uttar Pradesh, 
      201303, India. nchauhan1@amity.edu.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201204
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Adhesins, Bacterial)
RN  - 0 (Antibodies)
RN  - 0 (Antigens, Bacterial)
RN  - 0 (BabA protein, Helicobacter pylori)
RN  - 0 (Bridged Bicyclo Compounds, Heterocyclic)
RN  - 0 (Polymers)
RN  - 0 (graphene oxide)
RN  - 0 (poly(3,4-ethylene dioxythiophene))
RN  - 5TWQ1V240M (Palladium)
RN  - 7440-57-5 (Gold)
RN  - 7782-42-5 (Graphite)
SB  - IM
MH  - Adhesins, Bacterial/*immunology/metabolism
MH  - Antibodies
MH  - Antigens, Bacterial
MH  - Bridged Bicyclo Compounds, Heterocyclic/chemistry
MH  - Electrochemical Techniques/instrumentation/*methods
MH  - Electrodes
MH  - Gold/chemistry
MH  - Graphite/chemistry
MH  - Helicobacter pylori/*immunology/isolation & purification/metabolism
MH  - Hydrogen-Ion Concentration
MH  - Immunoassay/*methods
MH  - Metal Nanoparticles/chemistry
MH  - Microscopy, Electron, Scanning
MH  - Microscopy, Electron, Transmission
MH  - Molecular Docking Simulation
MH  - Nanostructures/*chemistry/ultrastructure
MH  - Palladium/chemistry
MH  - Polymers/chemistry
MH  - Temperature
PMC - PMC7719176
COIS- The authors declare no competing interests.
EDAT- 2020/12/06 06:00
MHDA- 2021/04/15 06:00
PMCR- 2020/12/04
CRDT- 2020/12/05 05:30
PHST- 2019/12/23 00:00 [received]
PHST- 2020/10/19 00:00 [accepted]
PHST- 2020/12/05 05:30 [entrez]
PHST- 2020/12/06 06:00 [pubmed]
PHST- 2021/04/15 06:00 [medline]
PHST- 2020/12/04 00:00 [pmc-release]
AID - 10.1038/s41598-020-78068-w [pii]
AID - 78068 [pii]
AID - 10.1038/s41598-020-78068-w [doi]
PST - epublish
SO  - Sci Rep. 2020 Dec 4;10(1):21217. doi: 10.1038/s41598-020-78068-w.