PMID- 33277833
OWN - NLM
STAT- MEDLINE
DCOM- 20210820
LR  - 20210820
IS  - 2241-6293 (Electronic)
IS  - 1107-0625 (Linking)
VI  - 25
IP  - 5
DP  - 2020 Sep-Oct
TI  - LncRNA-PCAT1 controls the growth, metastasis and drug resistance of human colon 
      cancer cells.
PG  - 2180-2185
AB  - PURPOSE: The long non-coding (lnc) RNAs have been shown to exhibit profound 
      regulatory roles in maintaining the growth and proliferation of human cancer 
      cells. Taking this fact into consideration, the current research work was 
      scheduled to explore the regulatory control of lncRNA-PCAT1 in maintaining the 
      growth and progression of human colon cancer cell. METHODS: The expression of 
      lncRNA-PCAT1 was assessed through qRT-PCR method. DAPI and acridine orange 
      (AO)/ethidium bromide (EB) staining protocols along with the colony formation 
      protocols were performed to evaluate the viability of cancer cells. The migratory 
      and invasion properties of cancer cells were examined by the wound-healing and 
      transwell assays, respectively. Western blotting was used to assess the 
      expression of proteins of interest. MTT assay was used for the assessment of cell 
      proliferation. RESULTS: lncRNA-PCAT1 was highly up-regulated in the colon cancer 
      tissues and cancer cell lines. The repression of lncRNA-PCAT1 in colon cancer 
      cells reduced their viability through induction of Bax/Bcl-2 mediated apoptosis. 
      The inhibition of lncRNA-PCAT1 expression further declined the migration and 
      invasion of colon cancer cells along with the decline of cell proliferation and 
      enhanced the chemosensitivity of colon cancer cells. CONCLUSION: lncRNA-PCAT1 
      expression may be utilized as a vital prognostic tool in colon cancer and 
      highlighted its regulatory effects in maintaining the colon cancer growth and 
      proliferation.
FAU - Sun, Haiying
AU  - Sun H
AD  - Department of Gastroenterology, the First Hospital of China Medical University, 
      Shenyang, Liaoning 110001, China.
FAU - Sun, Xuren
AU  - Sun X
FAU - Zhang, Huijing
AU  - Zhang H
FAU - Yue, An
AU  - Yue A
FAU - Sun, Mingjun
AU  - Sun M
LA  - eng
PT  - Journal Article
PL  - Cyprus
TA  - J BUON
JT  - Journal of B.U.ON. : official journal of the Balkan Union of Oncology
JID - 100883428
RN  - 0 (PCAT-1 lncRNA, human)
RN  - 0 (RNA, Long Noncoding)
SB  - IM
MH  - Apoptosis/physiology
MH  - Cell Movement/physiology
MH  - Cell Proliferation/physiology
MH  - Colonic Neoplasms/*drug therapy/genetics/*metabolism/pathology
MH  - Drug Resistance
MH  - Drug Resistance, Neoplasm
MH  - HCT116 Cells
MH  - Humans
MH  - Neoplasm Metastasis
MH  - RNA, Long Noncoding/*biosynthesis/genetics/metabolism
MH  - Transfection
MH  - Up-Regulation
EDAT- 2020/12/06 06:00
MHDA- 2021/08/21 06:00
CRDT- 2020/12/05 05:35
PHST- 2020/12/05 05:35 [entrez]
PHST- 2020/12/06 06:00 [pubmed]
PHST- 2021/08/21 06:00 [medline]
PST - ppublish
SO  - J BUON. 2020 Sep-Oct;25(5):2180-2185.