PMID- 33277838
OWN - NLM
STAT- MEDLINE
DCOM- 20210820
LR  - 20210820
IS  - 2241-6293 (Electronic)
IS  - 1107-0625 (Linking)
VI  - 25
IP  - 5
DP  - 2020 Sep-Oct
TI  - MicroRNA-1 regulates the growth and chemosensitivity of breast cancer cells by 
      targeting MEK/ERK pathway.
PG  - 2215-2220
AB  - PURPOSE: The microRNAs (miRs) control a vast number of biological and cellular 
      processes. miR-1 has been implicated in the development and progression in 
      different types of cancers. Nonetheless, the function and therapeutic 
      implications of miR-1 have not been studied in breast cancer. This study was 
      undertaken to study the role of miR-1 in human breast cancer cells. METHODS: 
      MBA-MD-231 breast cancer line and the normal MB-157 cell line were mainly used in 
      this research. Expression analysis was performed by qRT-PCR. Cell viability was 
      determined by MTT assay and apoptosis was detected by acridine orange 
      (AO)/ethidium bromide (EB) and DAPI staining. Transwell assay was used for cell 
      migration and invasion and western blot analysis was used to determine the 
      protein expression. RESULTS: miR-1 was significantly but aberrantly suppressed in 
      breast cancer cells relative to the MB-157 normal cells. Overexpression of miR-1 
      in MBA-MD-231 suppressed their proliferation dose-dependently. The inhibition of 
      MBA-MD-231 cell proliferation was found to be due to induction of apoptosis. The 
      apoptotic cell percentage was 37.1% in miR-1 mimics transfected in comparison to 
      3.7% in miR-negative control (NC) transfected cells. Additionally, miR-1 also 
      suppressed the chemosensitivity of the MBA-MD-1 breast cancer cells to cisplatin. 
      Transwell assay showed that miR-1 overexpression suppressed the migration and 
      invasion of the MBA-MD-231 cells. The results clearly showed that overexpression 
      of miR-1 suppressed the phosphorylation of MEK and ERK. CONCLUSION: miR-1 acts as 
      a tumor suppressor and may exhibit therapeutic implications in the treatment of 
      breast cancer.
FAU - Yang, Lihong
AU  - Yang L
AD  - Department of Nursing, Medical College of ChiFeng University, Chifeng, Inner 
      Mongolia 024000, China.
FAU - Cai, Nannan
AU  - Cai N
FAU - Zhao, Li
AU  - Zhao L
LA  - eng
PT  - Journal Article
PL  - Cyprus
TA  - J BUON
JT  - Journal of B.U.ON. : official journal of the Balkan Union of Oncology
JID - 100883428
RN  - 0 (MIRN1 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - EC 2.7.12.2 (Mitogen-Activated Protein Kinase Kinases)
SB  - IM
MH  - Apoptosis
MH  - Breast Neoplasms/genetics/*metabolism/pathology
MH  - Cell Survival
MH  - Female
MH  - Humans
MH  - *MAP Kinase Signaling System
MH  - MicroRNAs/genetics/*metabolism
MH  - Mitogen-Activated Protein Kinase Kinases/genetics/*metabolism
EDAT- 2020/12/06 06:00
MHDA- 2021/08/21 06:00
CRDT- 2020/12/05 05:35
PHST- 2020/12/05 05:35 [entrez]
PHST- 2020/12/06 06:00 [pubmed]
PHST- 2021/08/21 06:00 [medline]
PST - ppublish
SO  - J BUON. 2020 Sep-Oct;25(5):2215-2220.