PMID- 33277876
OWN - NLM
STAT- MEDLINE
DCOM- 20210820
LR  - 20210820
IS  - 2241-6293 (Electronic)
IS  - 1107-0625 (Linking)
VI  - 25
IP  - 5
DP  - 2020 Sep-Oct
TI  - CHAF1A overexpression in human retinoblastoma promotes cell proliferation and 
      suppresses apoptosis.
PG  - 2510-2514
AB  - PURPOSE: Retinoblastoma causes significant human mortality especially in 
      children. Although retinoblastoma may be treated if detected at early stage, 
      however, it becomes destructive at advanced stages. The treatment involves 
      surgery and chemotherapy. However, the chemotherapeutic agents have severe 
      adverse effects. Therefore, development of viable drugs and identification of 
      novel molecular therapeutic targets may enable efficient management of 
      retinoblastoma. This study was designed to examine the expression profile of 
      Chromatin Assembly Factor-1 (CHAF1A) and explore its therapeutic implications in 
      retinoblastoma. METHODS: The expression of CHAF1A was determined by qRT-PCR. MTT 
      assay was used for the determination of the cell viability. Apoptosis was 
      detected by acridine orange (AO)/ethidium bromide (EB) and annexin V/propidium 
      iodide (PI) assay. Cell cycle analysis was determined by flow cytometery. Protein 
      expression was determined by western blot analysis. RESULTS: The results showed 
      that CHAF1A is significantly upregulated in human retinoblastoma, with 7.3 folds 
      upregulation in retinoblastoma cells relative to normal cells. Knockdown of 
      CHAF1A resulted in significant decline in the viability of the RB355 
      retinoblastoma cells. The flow cytometric analysis showed that knockdown of 
      CHAF1A caused arrest of the RB355 cells at G0/G1 phase of the cell cycle. This 
      was also linked with significant downregulation of cyclin D1 and cyclin E1. The 
      AO/EB staining assay showed that CHAF1A knockdown promotes apoptosis which is 
      associated with downregulation of Bcl-2 and upregulation of Bax. CONCLUSION: 
      Taken together, these results suggest that CHAF1A is upregulated in 
      retinoblastoma and regulates its proliferation and apoptosis. As such CHAF1A may 
      act as biomarker as well as therapeutic target for the management of 
      retinoblastoma.
FAU - Shen, Jian
AU  - Shen J
AD  - Department of Ophthalmology, Affilated Zhongshan Hospital of Dalian University, 
      Dalian 116001, China.
FAU - Liu, Xudong
AU  - Liu X
FAU - Zhou, Ming
AU  - Zhou M
FAU - Liu, Haojie
AU  - Liu H
FAU - Xu, Linping
AU  - Xu L
FAU - Meng, Xiangjun
AU  - Meng X
LA  - eng
PT  - Journal Article
PL  - Cyprus
TA  - J BUON
JT  - Journal of B.U.ON. : official journal of the Balkan Union of Oncology
JID - 100883428
RN  - 0 (CHAF1A protein, human)
RN  - 0 (Chromatin Assembly Factor-1)
SB  - IM
MH  - Apoptosis
MH  - Cell Proliferation
MH  - Chromatin Assembly Factor-1/metabolism
MH  - Humans
MH  - Retinoblastoma/*genetics/pathology
EDAT- 2020/12/06 06:00
MHDA- 2021/08/21 06:00
CRDT- 2020/12/05 05:35
PHST- 2020/12/05 05:35 [entrez]
PHST- 2020/12/06 06:00 [pubmed]
PHST- 2021/08/21 06:00 [medline]
PST - ppublish
SO  - J BUON. 2020 Sep-Oct;25(5):2510-2514.