PMID- 33277984
OWN - NLM
STAT- MEDLINE
DCOM- 20211018
LR  - 20211018
IS  - 2053-1095 (Electronic)
IS  - 2053-1095 (Linking)
VI  - 7
IP  - 3
DP  - 2021 May
TI  - Genetic characterization of a novel recombined porcine reproductive and 
      respiratory syndrome virus 2 among Nadc30-like, Jxa1-like and TJ-like strains.
PG  - 697-704
LID - 10.1002/vms3.402 [doi]
AB  - Porcine reproductive and respiratory syndrome (PRRS) is one of the most 
      economically devastating viral diseases in the global pig industry, including 
      China. Recently, we successfully isolated a porcine reproductive and respiratory 
      syndrome virus (PRRSV) from lung tissue and peripheral blood of piglets at a farm 
      from Dujiangyan in Sichuan, China, and named it the DJY-19 strain. The 
      full-length genome sequence of DJY-19 shared 86.8%-94.1% nucleotide similarity 
      with NADC30-like and NADC30 PRRSV strains. We compared the open reading frame 
      (ORF) 5 gene of DJY-19 with 34 PRRSV strains from Genbank. Phylogenetic analysis 
      showed that DJY-19 clustered with NADC30 strains, characterized by a predicted 
      131-amino-acid deletion in the nonstructural protein (NSP) 2. The results of 
      homology analysis showed that the homology between DJY-19 and NADC30 (JN654459.1) 
      strains was the highest (95.9%), whereas homology with other domestic strains was 
      lower (80.9%-92.6%). Furthermore, we identified four recombination breakpoints in 
      the DJY-19 genome; they separated the DJY-19 genome into four regions. The 
      8106-9128 nucleotide (nt) region of DIY-19 was highly similar to the TJ strain, 
      and the 12106-12580 nt region of DIY-19 was highly similar to the JXA1-R strain. 
      Our findings demonstrate that DJY-19 arose from the recombination of North 
      America NADC30 strain and TJ strain and JXA1-R in China. The application of 
      multiple attenuated vaccine strains has led to complex recombination of PRRSV 
      strains in China. This study provides a theoretical basis for making a more 
      reasonable PRRS virus control and prevention strategy.
CI  - (c) 2020 The Authors Veterinary Medicine and Science Published by John Wiley & Sons 
      Ltd.
FAU - Zhao, Jun
AU  - Zhao J
AUID- ORCID: 0000-0002-5804-6816
AD  - College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.
FAU - Zhu, Ling
AU  - Zhu L
AD  - College of Veterinary Medicine, Key Laboratory of Animal Diseases and Human 
      Health of Sichuan Province, Sichuan Agricultural University, Chengdu, China.
FAU - Huang, Jianbo
AU  - Huang J
AD  - College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.
FAU - Yang, Zexiao
AU  - Yang Z
AD  - College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.
FAU - Xu, Lei
AU  - Xu L
AD  - College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.
FAU - Gu, Sirui
AU  - Gu S
AD  - College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.
FAU - Huang, Yao
AU  - Huang Y
AD  - College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.
FAU - Zhang, Rubo
AU  - Zhang R
AD  - College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.
FAU - Sun, Xiangang
AU  - Sun X
AD  - College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.
FAU - Zhou, Yuancheng
AU  - Zhou Y
AD  - Animal Breeding and Genetics Key Laboratory of Sichuan Province, Sichuan Animal 
      Science Academy, Chengdu, China.
FAU - Xu, Zhiwen
AU  - Xu Z
AD  - College of Veterinary Medicine, Key Laboratory of Animal Diseases and Human 
      Health of Sichuan Province, Sichuan Agricultural University, Chengdu, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201205
PL  - England
TA  - Vet Med Sci
JT  - Veterinary medicine and science
JID - 101678837
RN  - 0 (Viral Nonstructural Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - China
MH  - *Genome, Viral
MH  - Phylogeny
MH  - Porcine Reproductive and Respiratory Syndrome/virology
MH  - Porcine respiratory and reproductive syndrome virus/classification/*genetics
MH  - Sequence Alignment/veterinary
MH  - Sus scrofa
MH  - Swine
MH  - Viral Nonstructural Proteins/chemistry/*genetics/metabolism
PMC - PMC8136965
OTO - NOTNLM
OT  - genetic evolution analysis
OT  - new recombinant porcine reproductive and respiratory syndrome virus strain
OT  - reference basis
COIS- The authors declare that there is no conflict of interest.
EDAT- 2020/12/06 06:00
MHDA- 2021/10/21 06:00
PMCR- 2020/12/05
CRDT- 2020/12/05 08:34
PHST- 2020/11/06 00:00 [revised]
PHST- 2020/09/03 00:00 [received]
PHST- 2020/11/09 00:00 [accepted]
PHST- 2020/12/06 06:00 [pubmed]
PHST- 2021/10/21 06:00 [medline]
PHST- 2020/12/05 08:34 [entrez]
PHST- 2020/12/05 00:00 [pmc-release]
AID - VMS3402 [pii]
AID - 10.1002/vms3.402 [doi]
PST - ppublish
SO  - Vet Med Sci. 2021 May;7(3):697-704. doi: 10.1002/vms3.402. Epub 2020 Dec 5.