PMID- 33278673
OWN - NLM
STAT- MEDLINE
DCOM- 20201231
LR  - 20201231
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1635
DP  - 2021 Jan 4
TI  - Nanoparticle-based polyacrylonitrile monolithic column for highly efficient micro 
      solid-phase extraction of carotenoids and vitamins in human serum.
PG  - 461755
LID - S0021-9673(20)31029-3 [pii]
LID - 10.1016/j.chroma.2020.461755 [doi]
AB  - In this work, a biocompatible monolithic column based micro-solid-phase 
      extraction (micro-SPE) method was developed for biological fluid analysis. A novel 
      nanoparticle-based polyacrylonitrile monolithic column (C30 NP-PMC) was 
      fabricated by incorporating triacontyl (C30) modified silica nanoparticles (NPs) 
      into the polyacrylonitrile monolithic matrix through thermally induced phase 
      separation. With efficient mass transfer and sorption capacity, C30 NP-PMC 
      exhibited outstanding performance for the extraction of carotenoids and 
      fat-soluble vitamins (FSVs) from human serum samples, superior to commercial C18 
      cartridges as well as liquid-liquid extraction (LLE) method. Under optimal 
      conditions, the proposed micro-SPE method coupled with high-performance liquid 
      chromatography-diode array detection (HPLC-DAD) achieved satisfactory limits of 
      detection (LODs) (1.5-75.0 ng/mL) and good recoveries (85.0-106.5 %) with 
      relative standard deviations (RSDs) of less than 12.1% by consuming lower sorbent 
      (35.0 mg) and organic solvent (0.8 mL). Successful application of the developed 
      method demonstrated the great potential of such monolithic sorbents for efficient 
      isolation and preconcentration of trace analytes from blood samples.
CI  - Copyright (c) 2020. Published by Elsevier B.V.
FAU - Qi, Fei-Fei
AU  - Qi FF
AD  - School of Public Health, Xi'an Jiaotong University Health Science Center, Xi'an, 
      Shanxi, 710061, P.R. China.
FAU - Ma, Tian-You
AU  - Ma TY
AD  - School of Public Health, Xi'an Jiaotong University Health Science Center, Xi'an, 
      Shanxi, 710061, P.R. China.
FAU - Fan, Ya-Meng
AU  - Fan YM
AD  - School of Public Health, Xi'an Jiaotong University Health Science Center, Xi'an, 
      Shanxi, 710061, P.R. China.
FAU - Chu, Lan-Ling
AU  - Chu LL
AD  - School of Light Industry and Food Engineering, Nanjing Forestry University, 
      Nanjing, Jiangsu, 210037, P.R. China.
FAU - Liu, Yan
AU  - Liu Y
AD  - School of Public Health, Xi'an Jiaotong University Health Science Center, Xi'an, 
      Shanxi, 710061, P.R. China; Sichuan Provincial Center for Disease Control and 
      Prevention, Chengdu, Sichuan, 610041, P.R. China.
FAU - Yu, Yan
AU  - Yu Y
AD  - School of Public Health, Xi'an Jiaotong University Health Science Center, Xi'an, 
      Shanxi, 710061, P.R. China. Electronic address: yuyan@mail.xjtu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20201126
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Acrylic Resins)
RN  - 0 (Solvents)
RN  - 0 (Vitamins)
RN  - 25014-41-9 (polyacrylonitrile)
RN  - 36-88-4 (Carotenoids)
RN  - 7631-86-9 (Silicon Dioxide)
SB  - IM
MH  - Acrylic Resins/*chemistry
MH  - Blood Chemical Analysis/instrumentation/*methods
MH  - Carotenoids/blood/*isolation & purification
MH  - Chromatography, High Pressure Liquid
MH  - Humans
MH  - Limit of Detection
MH  - Nanoparticles/*chemistry
MH  - Silicon Dioxide
MH  - Solid Phase Extraction/*instrumentation
MH  - *Solid Phase Microextraction
MH  - Solvents
MH  - Vitamins/blood/*isolation & purification
OTO - NOTNLM
OT  - Biocompatibility
OT  - Carotenoids
OT  - Fat-soluble vitamins
OT  - Micro-solid phase extraction
OT  - Monolithic column
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2020/12/06 06:00
MHDA- 2021/01/01 06:00
CRDT- 2020/12/05 20:10
PHST- 2020/09/07 00:00 [received]
PHST- 2020/11/20 00:00 [revised]
PHST- 2020/11/22 00:00 [accepted]
PHST- 2020/12/06 06:00 [pubmed]
PHST- 2021/01/01 06:00 [medline]
PHST- 2020/12/05 20:10 [entrez]
AID - S0021-9673(20)31029-3 [pii]
AID - 10.1016/j.chroma.2020.461755 [doi]
PST - ppublish
SO  - J Chromatogr A. 2021 Jan 4;1635:461755. doi: 10.1016/j.chroma.2020.461755. Epub 
      2020 Nov 26.