PMID- 33279059
OWN - NLM
STAT- MEDLINE
DCOM- 20210531
LR  - 20210531
IS  - 1095-9998 (Electronic)
IS  - 0740-0020 (Linking)
VI  - 94
DP  - 2021 Apr
TI  - Biovolume and spatial distribution of foodborne Gram-negative and Gram-positive 
      pathogenic bacteria in mono- and dual-species biofilms.
PG  - 103616
LID - S0740-0020(20)30205-7 [pii]
LID - 10.1016/j.fm.2020.103616 [doi]
AB  - The objective of this study was to characterize the biofilms formed by Salmonella 
      enterica serotype Agona, Listeria monocytogenes, methicillin-resistant 
      Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus faecium (VRE) 
      after 12, 48, 72, 120 and 240 h of incubation at 10  degrees C. Biofilms containing a 
      single species, together with dual-species biofilms in which S. enterica and a 
      Gram-positive bacterium existed in combination, were formed on polystyrene and 
      evaluated by using confocal laser scanning microscopy (CLSM). All strains were 
      able to form biofilm. The greatest biovolume in the observation field of 
      14,161 mum(2) was observed for mono-species biofilms after 72 h, where biovolumes 
      of 94,409.0 mum(3) +/- 2131.0 mum(3) (S. enterica), 58,418.3 mum(3) +/- 5944.9 mum(3) (L. 
      monocytogenes), 68,020.8 mum(3) +/- 5812.3 mum(3) (MRSA) and 
      59,280.0 mum(3) +/- 4032.9 mum(3) (VRE) were obtained. In comparison with 
      single-species biofilms, the biovolume of S. enterica was higher in the presence 
      of MRSA or VRE after 48, 72 and 120 h. In dual-species biofilms, the bacteria 
      showed a double-layer distribution pattern, with S. enterica in the top layer and 
      Gram-positive bacteria in the bottom layer. This spatial disposition should be 
      taken into account when effective strategies to eliminate biofilms are being 
      developed.
CI  - Copyright (c) 2020 Elsevier Ltd. All rights reserved.
FAU - Rodriguez-Melcon, Cristina
AU  - Rodriguez-Melcon C
AD  - Department of Food Hygiene and Technology, Veterinary Faculty, University of 
      Leon, E-24071, Leon, Spain; Institute of Food Science and Technology, University 
      of Leon, E-24071, Leon, Spain.
FAU - Alonso-Hernando, Alicia
AU  - Alonso-Hernando A
AD  - Department of Food Hygiene and Technology, Veterinary Faculty, University of 
      Leon, E-24071, Leon, Spain; Facultad de Ciencias de la Salud, Universidad Isabel 
      I, E-09003, Burgos, Spain.
FAU - Riesco-Pelaez, Felix
AU  - Riesco-Pelaez F
AD  - Department of Electrical Engineering and Systems Engineering and Automatic 
      Control, University of Leon, E-24071, Leon, Spain.
FAU - Garcia-Fernandez, Camino
AU  - Garcia-Fernandez C
AD  - Department of Food Hygiene and Technology, Veterinary Faculty, University of 
      Leon, E-24071, Leon, Spain; Institute of Food Science and Technology, University 
      of Leon, E-24071, Leon, Spain.
FAU - Alonso-Calleja, Carlos
AU  - Alonso-Calleja C
AD  - Department of Food Hygiene and Technology, Veterinary Faculty, University of 
      Leon, E-24071, Leon, Spain; Institute of Food Science and Technology, University 
      of Leon, E-24071, Leon, Spain.
FAU - Capita, Rosa
AU  - Capita R
AD  - Department of Food Hygiene and Technology, Veterinary Faculty, University of 
      Leon, E-24071, Leon, Spain; Institute of Food Science and Technology, University 
      of Leon, E-24071, Leon, Spain. Electronic address: rosa.capita@unileon.es.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20200808
PL  - England
TA  - Food Microbiol
JT  - Food microbiology
JID - 8601127
SB  - IM
MH  - *Biofilms
MH  - Enterococcus faecium/*chemistry/physiology
MH  - Listeria monocytogenes/*chemistry/physiology
MH  - Methicillin-Resistant Staphylococcus aureus/*chemistry/physiology
MH  - Microscopy, Confocal
MH  - Salmonella enterica/*chemistry/physiology
OTO - NOTNLM
OT  - Biofilm architecture
OT  - Dual-species biofilms
OT  - Listeria monocytogenes
OT  - MRSA
OT  - Salmonella enterica
OT  - Single-species biofilms
OT  - VRE
EDAT- 2020/12/07 06:00
MHDA- 2021/06/01 06:00
CRDT- 2020/12/06 20:39
PHST- 2020/05/04 00:00 [received]
PHST- 2020/07/23 00:00 [revised]
PHST- 2020/07/31 00:00 [accepted]
PHST- 2020/12/06 20:39 [entrez]
PHST- 2020/12/07 06:00 [pubmed]
PHST- 2021/06/01 06:00 [medline]
AID - S0740-0020(20)30205-7 [pii]
AID - 10.1016/j.fm.2020.103616 [doi]
PST - ppublish
SO  - Food Microbiol. 2021 Apr;94:103616. doi: 10.1016/j.fm.2020.103616. Epub 2020 Aug 
      8.