PMID- 33281889
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201208
IS  - 1684-5315 (Print)
IS  - 1684-5315 (Electronic)
IS  - 1684-5315 (Linking)
VI  - 16
IP  - 36
DP  - 2017
TI  - Phylogenetic characterization of East African cassava mosaic begomovirus 
      (Geminiviridae) isolated from Manihot carthaginensis subsp. glaziovii (Mull.Arg.) 
      Allem., from a non-cassava growing region in Tanzania.
LID - 10.5897/AJB2017.16130 [doi]
AB  - Manihot carthaginensis subsp. glaziovii (Mull.Arg.) Allem., a wild relative of 
      cassava, native to Brazil, is one of the popular agroforestry trees used for 
      hedges and/or boundary plants surrounding homesteads and farms and also harbours 
      cassava mosaic begomoviruses (CMBs) and cassava brown streak ipomoviruses. 
      Sequences of the DNA-A component of East African cassava mosaic virus (EACMV) 
      isolates from M. carthaginensis subsp. glaziovii (Mull.Arg.) Allem., collected 
      from non-cassava growing areas of Tanzania were characterized. Thirteen full 
      length DNA-A sequences were analysed together with 15 already reported EACMV 
      sequences and six CMB species reference genomes. The results show 96 to 100% 
      nucleotide sequence identity with EACMV isolates from Kenya. Phylogenetic 
      analysis revealed that EACMV isolates from M. carthaginensis subsp. glaziovii 
      (Mull.Arg.) Allem, belong to a single cassava mosaic begomovirus species. The 
      EACMV monophyletic clade is distinct from all other CMB species. The presence of 
      Cassava infecting begomoviruses in wild cassava relative growing from 
      traditionally non cassava growing region serve as inoculum sources for 
      cassava-infecting begomoviruses and therefore their eradication is key in the 
      sustainable management of CMBs, especially in the non-cassava growing areas.
CI  - (c) 2017 Author(s) retain the copyright of this article.
FAU - Tairo, F
AU  - Tairo F
AD  - Mikocheni Agricultural Research Institute, P.O Box 6226, Dar es Salaam, Tanzania.
FAU - Mbewe, W K
AU  - Mbewe WK
AD  - School of Agricultural Sciences, Makerere University, P. O. Box 7062, Kampala, 
      Uganda.
AD  - Bvumbwe Agricultural Research Station, P. O. Box 5748, Limbe, Malawi.
FAU - Mark, D
AU  - Mark D
AD  - Mikocheni Agricultural Research Institute, P.O Box 6226, Dar es Salaam, Tanzania.
FAU - Lupembe, M
AU  - Lupembe M
AD  - Mikocheni Agricultural Research Institute, P.O Box 6226, Dar es Salaam, Tanzania.
FAU - Sseruwagi, P
AU  - Sseruwagi P
AD  - Mikocheni Agricultural Research Institute, P.O Box 6226, Dar es Salaam, Tanzania.
FAU - Ndunguru, J
AU  - Ndunguru J
AD  - Mikocheni Agricultural Research Institute, P.O Box 6226, Dar es Salaam, Tanzania.
LA  - eng
PT  - Journal Article
DEP - 20170906
PL  - Kenya
TA  - Afr J Biotechnol
JT  - African journal of biotechnology
JID - 101186077
PMC - PMC7691754
OTO - NOTNLM
OT  - Cassava mosaic disease
OT  - East African cassava mosaic virus
OT  - Manihot carthaginensis subsp. glaziovii (Mull.Arg.) Allem.
OT  - genetic diversity
COIS- The authors have not declared any conflict of interests.
EDAT- 2017/01/01 00:00
MHDA- 2017/01/01 00:01
PMCR- 2020/11/27
CRDT- 2020/12/07 05:31
PHST- 2017/06/30 00:00 [received]
PHST- 2017/08/09 00:00 [accepted]
PHST- 2020/12/07 05:31 [entrez]
PHST- 2017/01/01 00:00 [pubmed]
PHST- 2017/01/01 00:01 [medline]
PHST- 2020/11/27 00:00 [pmc-release]
AID - AJB-16-36-1826 [pii]
AID - 10.5897/AJB2017.16130 [doi]
PST - ppublish
SO  - Afr J Biotechnol. 2017;16(36). doi: 10.5897/AJB2017.16130. Epub 2017 Sep 6.