PMID- 33282514
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201208
IS  - 2156-7085 (Print)
IS  - 2156-7085 (Electronic)
IS  - 2156-7085 (Linking)
VI  - 11
IP  - 11
DP  - 2020 Nov 1
TI  - Erythrocyte volumetric measurements in imaging flow cytometry using simultaneous 
      three-wavelength digital holographic microscopy.
PG  - 6649-6658
LID - 10.1364/BOE.404368 [doi]
AB  - We report a cross-talk free simultaneous three-wavelength digital holographic 
      microscopy setup for spectroscopic imaging of biological cells during flow. The 
      feasibility of the proposed measurement technique is demonstrated on 
      erythrocytes, due to their unique morphology and dependency of hemoglobin (Hb) 
      molecule absorption on wavelength. From the spectroscopic quantitative phase 
      profiles of cells acquired during flow in a microfluidic device, we decoupled the 
      refractive index and the physical thickness. We then used our quantitative phase 
      imaging results to dynamically calculate the mean cell volume (MCV), mean 
      corpuscular Hb concentration (MCHC), mean corpuscular Hb content (MCH) and 
      sphericity index.
CI  - (c) 2020 Optical Society of America under the terms of the OSA Open Access 
      Publishing Agreement.
FAU - Turko, Nir A
AU  - Turko NA
AD  - Department of Biomedical Engineering, Faculty of Engineering, Tel-Aviv 
      University, Tel-Aviv 69978, Israel.
FAU - Shaked, Natan T
AU  - Shaked NT
AD  - Department of Biomedical Engineering, Faculty of Engineering, Tel-Aviv 
      University, Tel-Aviv 69978, Israel.
LA  - eng
PT  - Journal Article
DEP - 20201022
PL  - United States
TA  - Biomed Opt Express
JT  - Biomedical optics express
JID - 101540630
PMC - PMC7687930
COIS- NTS and NAT: Tel Aviv University (P).
EDAT- 2020/12/08 06:00
MHDA- 2020/12/08 06:01
PMCR- 2020/11/01
CRDT- 2020/12/07 05:34
PHST- 2020/08/03 00:00 [received]
PHST- 2020/09/30 00:00 [revised]
PHST- 2020/09/30 00:00 [accepted]
PHST- 2020/12/07 05:34 [entrez]
PHST- 2020/12/08 06:00 [pubmed]
PHST- 2020/12/08 06:01 [medline]
PHST- 2020/11/01 00:00 [pmc-release]
AID - 404368 [pii]
AID - 10.1364/BOE.404368 [doi]
PST - epublish
SO  - Biomed Opt Express. 2020 Oct 22;11(11):6649-6658. doi: 10.1364/BOE.404368. 
      eCollection 2020 Nov 1.