PMID- 33283328
OWN - NLM
STAT- MEDLINE
DCOM- 20211029
LR  - 20211029
IS  - 1098-2825 (Electronic)
IS  - 0887-8013 (Print)
IS  - 0887-8013 (Linking)
VI  - 35
IP  - 2
DP  - 2021 Feb
TI  - Interpretation of the HLA epitopes-Continuous dilution method for detection of 
      high-titer IgG HLA antibody.
PG  - e23632
LID - 10.1002/jcla.23632 [doi]
LID - e23632
AB  - BACKGROUND: The presence or absence of human leukocyte antigen (HLA) antibodies, 
      especially the strength of donor-specific HLA antibodies (DSAs), has important 
      roles in clinical evaluation and diagnostic decision-making for solid-organ 
      transplantation. Dilution patterns help to give a new sight of HLA epitopes. 
      "Epitope matching" is likely to lower the risk of developing DSA and increase the 
      likelihood of matching a compatible donor. METHODS: We collected data evaluating 
      HLA antibodies with a titration study using mean fluorescence intensity. RESULTS: 
      Diluting the serum of recipients can reduce potential inhibitory effects, 
      accurately evaluate the intensity of donor-specific HLA antibodies, and guide 
      surgeons to take or not take intervention measures. Dilution patterns also help 
      to give a new sight of HLA epitopes. CONCLUSION: We believe that from the 
      viewpoint of HLA antibodies, the dilution model can provide new tools and 
      insights for the study of HLA epitopes.
CI  - (c) 2020 The Authors. Journal of Clinical Laboratory Analysis published by Wiley 
      Periodicals LLC.
FAU - Xiao, Yanli
AU  - Xiao Y
AUID- ORCID: 0000-0002-9193-8541
AD  - Department of Blood Transfusion, Tianjin First Central Hospital, Tianjin, China.
FAU - Liu, Wei
AU  - Liu W
AD  - Department of Blood Transfusion, Tianjin First Central Hospital, Tianjin, China.
FAU - Kang, Zhongyu
AU  - Kang Z
AUID- ORCID: 0000-0002-6855-8153
AD  - Department of Blood Transfusion, Tianjin First Central Hospital, Tianjin, China.
FAU - Li, Daihong
AU  - Li D
AD  - Department of Blood Transfusion, Tianjin First Central Hospital, Tianjin, China.
LA  - eng
PT  - Case Reports
DEP - 20201201
PL  - United States
TA  - J Clin Lab Anal
JT  - Journal of clinical laboratory analysis
JID - 8801384
RN  - 0 (Epitopes)
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-DQ Antigens)
RN  - 0 (Immunoglobulin G)
SB  - IM
MH  - Allografts
MH  - Epitopes/*immunology
MH  - HLA Antigens/*blood/immunology
MH  - HLA-DQ Antigens/blood/immunology
MH  - Histocompatibility Testing/*methods
MH  - Humans
MH  - Immunoglobulin G/*blood
MH  - Kidney Transplantation
MH  - Male
MH  - Middle Aged
MH  - Tissue Donors
PMC - PMC7891501
OTO - NOTNLM
OT  - donor-specific HLA antibodies
OT  - epitope
OT  - titration study
EDAT- 2020/12/08 06:00
MHDA- 2021/10/30 06:00
PMCR- 2020/12/01
CRDT- 2020/12/07 05:40
PHST- 2020/06/02 00:00 [received]
PHST- 2020/09/23 00:00 [revised]
PHST- 2020/10/03 00:00 [accepted]
PHST- 2020/12/08 06:00 [pubmed]
PHST- 2021/10/30 06:00 [medline]
PHST- 2020/12/07 05:40 [entrez]
PHST- 2020/12/01 00:00 [pmc-release]
AID - JCLA23632 [pii]
AID - 10.1002/jcla.23632 [doi]
PST - ppublish
SO  - J Clin Lab Anal. 2021 Feb;35(2):e23632. doi: 10.1002/jcla.23632. Epub 2020 Dec 1.