PMID- 33284601
OWN - NLM
STAT- MEDLINE
DCOM- 20210218
LR  - 20210218
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 93
IP  - 2
DP  - 2021 Jan 19
TI  - Plasmonic SERS Biosensor Based on Multibranched Gold Nanoparticles Embedded in 
      Polydimethylsiloxane for Quantification of Hematin in Human Erythrocytes.
PG  - 1025-1032
LID - 10.1021/acs.analchem.0c03921 [doi]
AB  - This work reports a plasmonic surface-enhanced Raman scattering (SERS) biosensor 
      that allows for quantitative analysis of hematin in erythrocytes without the need 
      of separating it from hemoglobin (Hb). The biosensor exploits the tunable 
      localized surface plasmon resonance (LSPR) characteristics of multibranched gold 
      nanoparticles (M-AuNPs) and the strong plasmon coupling between an Au thin film 
      and a flexible substrate consisting of M-AuNPs embedded in polydimethylsiloxane 
      (PDMS) (i.e., M-AuNP-embedded PDMS substrate). In the assay, the hematin (or 
      hematin-containing erythrocyte hemolysate) was deposited on Au film surface and 
      covered with M-AuNP-embedded PDMS. Strong SERS signals were generated under 
      excitation at 785 nm; the signals were sensitive to hematin concentration but not 
      to several common coexisting biological substances. The intensities of the SERS 
      signal (at 1623 cm(-1)) displayed a wide linear range using hematin 
      concentrations in a range of at least  approximately 1.5 nM-1.1 muM; the limit of detection 
      (LOD) was  approximately 0.03 +/- 0.01 nM at a signal/noise (S/N) of 3. This assay is simple and 
      sensitive without tedious separation procedures, thereby saving time and 
      enhancing efficiency. This biosensor can be used to determine hematin 
      concentration in human erythrocyte cytosols giving concentrations of  approximately 18.5 +/- 4.5 
      (by averaging eight samples) and 51.5 +/- 6.2 muM (by averaging three samples) for 
      healthy and sickle erythrocytes, respectively, making it a potential application 
      in clinical detection.
FAU - Cai, Zhewei
AU  - Cai Z
AD  - Department of Chemical and Biomolecular Engineering, Clarkson University, 
      Potsdam, New York 13699, United States.
AD  - Jiangsu Key Laboratory of New Power Batteries, Jiangsu Collaborative Innovation 
      Center of Biomedical Functional Materials, College of Chemistry and Materials 
      Science, Nanjing Normal University, Nanjing 210023, P. R. China.
FAU - Hu, Yaojuan
AU  - Hu Y
AD  - School of Environmental Science, Nanjing Xiaozhuang University, Nanjing 211171, 
      P. R. China.
FAU - Sun, Yujie
AU  - Sun Y
AD  - Jiangsu Key Laboratory of New Power Batteries, Jiangsu Collaborative Innovation 
      Center of Biomedical Functional Materials, College of Chemistry and Materials 
      Science, Nanjing Normal University, Nanjing 210023, P. R. China.
FAU - Gu, Qingyu
AU  - Gu Q
AD  - Jiangsu Key Laboratory of New Power Batteries, Jiangsu Collaborative Innovation 
      Center of Biomedical Functional Materials, College of Chemistry and Materials 
      Science, Nanjing Normal University, Nanjing 210023, P. R. China.
FAU - Wu, Ping
AU  - Wu P
AD  - Jiangsu Key Laboratory of New Power Batteries, Jiangsu Collaborative Innovation 
      Center of Biomedical Functional Materials, College of Chemistry and Materials 
      Science, Nanjing Normal University, Nanjing 210023, P. R. China.
FAU - Cai, Chenxin
AU  - Cai C
AUID- ORCID: 0000-0002-3578-2415
AD  - Jiangsu Key Laboratory of New Power Batteries, Jiangsu Collaborative Innovation 
      Center of Biomedical Functional Materials, College of Chemistry and Materials 
      Science, Nanjing Normal University, Nanjing 210023, P. R. China.
FAU - Yan, Zijie
AU  - Yan Z
AUID- ORCID: 0000-0003-0726-7042
AD  - Department of Applied Physical Sciences, University of North Carolina at Chapel 
      Hill, Chapel Hill, North Carolina 27599, United States.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201207
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Dimethylpolysiloxanes)
RN  - 63148-62-9 (baysilon)
RN  - 743LRP9S7N (Hemin)
RN  - 7440-57-5 (Gold)
SB  - IM
MH  - *Biosensing Techniques
MH  - Dimethylpolysiloxanes/*chemistry
MH  - Erythrocytes/*chemistry
MH  - Gold/*chemistry
MH  - Hemin/*analysis
MH  - Humans
MH  - Metal Nanoparticles/*chemistry
MH  - Particle Size
MH  - Spectrum Analysis, Raman
MH  - Surface Properties
EDAT- 2020/12/08 06:00
MHDA- 2021/02/20 06:00
CRDT- 2020/12/07 17:10
PHST- 2020/12/08 06:00 [pubmed]
PHST- 2021/02/20 06:00 [medline]
PHST- 2020/12/07 17:10 [entrez]
AID - 10.1021/acs.analchem.0c03921 [doi]
PST - ppublish
SO  - Anal Chem. 2021 Jan 19;93(2):1025-1032. doi: 10.1021/acs.analchem.0c03921. Epub 
      2020 Dec 7.