PMID- 33287231
OWN - NLM
STAT- MEDLINE
DCOM- 20210624
LR  - 20210624
IS  - 2073-4409 (Electronic)
IS  - 2073-4409 (Linking)
VI  - 9
IP  - 12
DP  - 2020 Dec 3
TI  - The Interplay among Subunit Composition, Cardiolipin Content, and Aggregation 
      State of Bovine Heart Cytochrome c Oxidase.
LID - 10.3390/cells9122588 [doi]
LID - 2588
AB  - Mitochondrial cytochrome c oxidase (CcO) is a multisubunit integral membrane 
      complex consisting of 13 dissimilar subunits, as well as three to four tightly 
      bound molecules of cardiolipin (CL). The monomeric unit of CcO is able to form a 
      dimer and participate in the formation of supercomplexes in the inner 
      mitochondrial membrane. The structural and functional integrity of the enzyme is 
      crucially dependent on the full subunit complement and the presence of 
      unperturbed bound CL. A direct consequence of subunit loss, CL removal, or its 
      oxidative modification is the destabilization of the quaternary structure, loss 
      of the activity, and the inability to dimerize. Thus, the intimate interplay 
      between individual components of the complex is imperative for regulation of the 
      CcO aggregation state. While it appears that the aggregation state of CcO might 
      affect its conformational stability, the functional role of the aggregation 
      remains unclear as both monomeric and dimeric forms of CcO seem to be fully 
      active. Here, we review the current status of our knowledge with regard to the 
      role of dimerization in the function and stability of CcO and factors, such as 
      subunit composition, amphiphilic environment represented by 
      phospholipids/detergents, and posttranslational modifications that play a role in 
      the regulation of the CcO aggregation state.
FAU - Sedlak, Erik
AU  - Sedlak E
AUID- ORCID: 0000-0003-1290-5774
AD  - Center for Interdisciplinary Biosciences, P. J. Safarik University, Jesenna 5, 
      040 01 Kosice, Slovakia.
FAU - Kozar, Tibor
AU  - Kozar T
AD  - Center for Interdisciplinary Biosciences, P. J. Safarik University, Jesenna 5, 
      040 01 Kosice, Slovakia.
FAU - Musatov, Andrey
AU  - Musatov A
AUID- ORCID: 0000-0001-8991-2468
AD  - Department of Biophysics, Institute of Experimental Physics, Watsonova 47, 040 01 
      Kosice, Slovakia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
DEP - 20201203
PL  - Switzerland
TA  - Cells
JT  - Cells
JID - 101600052
RN  - 0 (Cardiolipins)
RN  - 0 (Phospholipids)
RN  - 0 (Protein Subunits)
RN  - EC 1.9.3.1 (Electron Transport Complex IV)
SB  - IM
MH  - Animals
MH  - Cardiolipins/*metabolism
MH  - Electron Transport Complex IV/*metabolism
MH  - Heart/*physiology
MH  - Humans
MH  - Mitochondria/metabolism
MH  - Phospholipids/metabolism
MH  - Protein Subunits/*metabolism
PMC - PMC7761698
OTO - NOTNLM
OT  - aggregations state
OT  - cardiolipin
OT  - cytochrome c oxidase
OT  - posttranslational modifications
COIS- The authors declare no conflict of interest.
EDAT- 2020/12/09 06:00
MHDA- 2021/06/25 06:00
PMCR- 2020/12/03
CRDT- 2020/12/08 01:07
PHST- 2020/10/28 00:00 [received]
PHST- 2020/11/24 00:00 [revised]
PHST- 2020/12/01 00:00 [accepted]
PHST- 2020/12/08 01:07 [entrez]
PHST- 2020/12/09 06:00 [pubmed]
PHST- 2021/06/25 06:00 [medline]
PHST- 2020/12/03 00:00 [pmc-release]
AID - cells9122588 [pii]
AID - cells-09-02588 [pii]
AID - 10.3390/cells9122588 [doi]
PST - epublish
SO  - Cells. 2020 Dec 3;9(12):2588. doi: 10.3390/cells9122588.