PMID- 33289106
OWN - NLM
STAT- Publisher
LR  - 20240222
IS  - 1938-3673 (Electronic)
IS  - 0741-5400 (Linking)
DP  - 2020 Dec 2
TI  - Comparison of the PU.1 transcriptional regulome and interactome in human and 
      mouse inflammatory dendritic cells.
LID - 10.1002/JLB.6A1219-711RRR [doi]
AB  - Dendritic cells (DCs) are key immune modulators and are able to mount immune 
      responses or tolerance. DC differentiation and activation imply a plethora of 
      molecular and cellular responses, including transcriptional changes. PU.1 is a 
      highly expressed transcription factor in DCs and coordinates relevant aspects of 
      DC biology. Due to their role as immune regulators, DCs pose as a promising 
      immunotherapy tool. However, some of their functional features, such as survival, 
      activation, or migration, are compromised due to the limitations to simulate in 
      vitro the physiologic DC differentiation process. A better knowledge of 
      transcriptional programs would allow the identification of potential targets for 
      manipulation with the aim of obtaining "qualified" DCs for immunotherapy 
      purposes. Most of the current knowledge regarding DC biology derives from studies 
      using mouse models, which not always find a parallel in human. In the present 
      study, we dissect the PU.1 transcriptional regulome and interactome in mouse and 
      human DCs, in the steady state or LPS activated. The PU.1 transcriptional 
      regulome was identified by performing PU.1 chromatin immunoprecipitation followed 
      by high-throughput sequencing and pairing these data with RNAsequencing data. The 
      PU.1 interactome was identified by performing PU.1 immunoprecipitation followed 
      by mass spectrometry analysis. Our results portray PU.1 as a pivotal factor that 
      plays an important role in the regulation of genes required for proper DC 
      activation and function, and assures the repression of nonlineage genes. The 
      interspecies differences between human and mouse DCs are surprisingly 
      substantial, highlighting the need to study the biology of human DCs.
CI  - (c)2020 Society for Leukocyte Biology.
FAU - Scheenstra, Maaike R
AU  - Scheenstra MR
AD  - Department of Blood Cell Research, Sanquin Research and Landsteiner Laboratory, 
      Academic Medical Center (AMC), University of Amsterdam, Amsterdam, The 
      Netherlands.
AD  - Division of Infectious Diseases and Immunology, Department of Biomolecular Health 
      Sciences, Utrecht University, Utrecht, The Netherlands.
FAU - Martinez-Botia, Patricia
AU  - Martinez-Botia P
AD  - Instituto de Investigacion Sanitaria del Principado de Asturias (ISPA), Oviedo, 
      Spain.
FAU - Acebes-Huerta, Andrea
AU  - Acebes-Huerta A
AD  - Instituto de Investigacion Sanitaria del Principado de Asturias (ISPA), Oviedo, 
      Spain.
FAU - Brouwer, Rutger W W
AU  - Brouwer RWW
AD  - Center for Biomics, Erasmus MC, Rotterdam, The Netherlands.
AD  - Department of Cell Biology, Erasmus MC, Rotterdam, The Netherlands.
FAU - Caballero-Sanchez, Noemi
AU  - Caballero-Sanchez N
AD  - Instituto de Investigacion Sanitaria del Principado de Asturias (ISPA), Oviedo, 
      Spain.
FAU - Gillemans, Nynke
AU  - Gillemans N
AD  - Department of Cell Biology, Erasmus MC, Rotterdam, The Netherlands.
FAU - De Bleser, Pieter
AU  - De Bleser P
AD  - Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium.
AD  - VIB Center for Inflammation Research, Data Mining and Modeling for Biomedicine, 
      Ghent, Belgium.
FAU - Nota, Benjamin
AU  - Nota B
AD  - Department of Blood Cell Research, Sanquin Research and Landsteiner Laboratory, 
      Academic Medical Center (AMC), University of Amsterdam, Amsterdam, The 
      Netherlands.
FAU - De Cuyper, Iris M
AU  - De Cuyper IM
AD  - Department of Blood Cell Research, Sanquin Research and Landsteiner Laboratory, 
      Academic Medical Center (AMC), University of Amsterdam, Amsterdam, The 
      Netherlands.
FAU - Salunkhe, Vishal
AU  - Salunkhe V
AD  - Department of Blood Cell Research, Sanquin Research and Landsteiner Laboratory, 
      Academic Medical Center (AMC), University of Amsterdam, Amsterdam, The 
      Netherlands.
FAU - Woltman, Andrea M
AU  - Woltman AM
AD  - Department of Gastroenterology and Hepatology, Erasmus MC, Rotterdam, The 
      Netherlands.
AD  - Current Address: Institute of Medical Education Research Rotterdam, Erasmus MC, 
      Rotterdam, The Netherlands.
FAU - van de Laar, Lianne
AU  - van de Laar L
AD  - Department of Gastroenterology and Hepatology, Erasmus MC, Rotterdam, The 
      Netherlands.
FAU - Rijkers, Erikjan
AU  - Rijkers E
AD  - Proteomics Center, Erasmus MC, Rotterdam, The Netherlands.
FAU - Demmers, Jeroen A A
AU  - Demmers JAA
AD  - Proteomics Center, Erasmus MC, Rotterdam, The Netherlands.
FAU - van IJcken, Wilfred F J
AU  - van IJcken WFJ
AUID- ORCID: 0000-0002-0421-8301
AD  - Center for Biomics, Erasmus MC, Rotterdam, The Netherlands.
AD  - Department of Cell Biology, Erasmus MC, Rotterdam, The Netherlands.
FAU - Philipsen, Sjaak
AU  - Philipsen S
AD  - Department of Cell Biology, Erasmus MC, Rotterdam, The Netherlands.
FAU - van den Berg, Timo K
AU  - van den Berg TK
AD  - Department of Blood Cell Research, Sanquin Research and Landsteiner Laboratory, 
      Academic Medical Center (AMC), University of Amsterdam, Amsterdam, The 
      Netherlands.
FAU - Kuijpers, Taco W
AU  - Kuijpers TW
AD  - Department of Blood Cell Research, Sanquin Research and Landsteiner Laboratory, 
      Academic Medical Center (AMC), University of Amsterdam, Amsterdam, The 
      Netherlands.
AD  - Laboratory of Immunotherapy, Sanquin Research and Landsteiner Laboratory, 
      Amsterdam University Medical Center, University of Amsterdam, Amsterdam, The 
      Netherlands.
AD  - Department of Molecular Cell Biology and Immunology, Amsterdam University Medical 
      Center, Vrije University, Amsterdam, The Netherlands.
FAU - Gutierrez, Laura
AU  - Gutierrez L
AUID- ORCID: 0000-0001-8443-900X
AD  - Department of Blood Cell Research, Sanquin Research and Landsteiner Laboratory, 
      Academic Medical Center (AMC), University of Amsterdam, Amsterdam, The 
      Netherlands.
AD  - Instituto de Investigacion Sanitaria del Principado de Asturias (ISPA), Oviedo, 
      Spain.
AD  - University of Oviedo, Oviedo, Spain.
LA  - eng
GR  - VENI 863.09.012/Netherlands Scientific Organization/
GR  - RYC-2013-12587/Spanish Ministerio de Economia y Competitividad/
GR  - PA-20-PF-BP19-014/Co nsejeria de Ciencia, Innovacion y Universidad del Principado 
      de Asturias (Spain)/
GR  - Fundacion para la Investigacion y la Innovacion Biosanitaria de Asturias (FINBA, 
      Spain)/
PT  - Journal Article
DEP - 20201202
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
SB  - IM
OTO - NOTNLM
OT  - PU.1
OT  - dendritic cells
OT  - interactome
OT  - interspecies
OT  - transcription factor
OT  - transcriptome
EDAT- 2020/12/09 06:00
MHDA- 2020/12/09 06:00
CRDT- 2020/12/08 05:46
PHST- 2019/12/11 00:00 [received]
PHST- 2020/11/18 00:00 [revised]
PHST- 2020/11/18 00:00 [accepted]
PHST- 2020/12/08 05:46 [entrez]
PHST- 2020/12/09 06:00 [pubmed]
PHST- 2020/12/09 06:00 [medline]
AID - 10.1002/JLB.6A1219-711RRR [doi]
PST - aheadofprint
SO  - J Leukoc Biol. 2020 Dec 2. doi: 10.1002/JLB.6A1219-711RRR.