PMID- 33289530
OWN - NLM
STAT- MEDLINE
DCOM- 20210610
LR  - 20210610
IS  - 1471-9053 (Electronic)
IS  - 0032-0781 (Linking)
VI  - 62
IP  - 1
DP  - 2021 Mar 25
TI  - Overproduction of Chloroplast Glyceraldehyde-3-Phosphate Dehydrogenase Improves 
      Photosynthesis Slightly under Elevated [CO2] Conditions in Rice.
PG  - 156-165
LID - 10.1093/pcp/pcaa149 [doi]
AB  - Chloroplast glyceraldehyde-3-phosphate dehydrogenase (GAPDH) limits the 
      regeneration of ribulose 1,5-bisphosphate (RuBP) in the Calvin-Benson cycle. 
      However, it does not always limit the rate of CO2 assimilation. In the present 
      study, the effects of overproduction of GAPDH on the rate of CO2 assimilation 
      under elevated [CO2] conditions, where the capacity for RuBP regeneration limits 
      photosynthesis, were examined in transgenic rice (Oryza sativa). GAPDH activity 
      was increased to 3.2- and 4.5-fold of the wild-type levels by co-overexpression 
      of the GAPDH genes, GAPA and GAPB, respectively. In the transgenic rice plants, 
      the rate of CO2 assimilation under elevated [CO2] conditions increased by 
      approximately 10%, whereas that under normal and low [CO2] conditions was not 
      affected. These results indicate that overproduction of GAPDH is effective in 
      improving photosynthesis under elevated [CO2] conditions, although its magnitude 
      is relatively small. By contrast, biomass production of the transgenic rice 
      plants was not greater than that of wild-type plants under elevated [CO2] 
      conditions, although starch content tended to increase marginally.
CI  - � The Author(s) 2020. Published by Oxford University Press on behalf of Japanese 
      Society of Plant Physiologists. All rights reserved. For permissions, please 
      email: journals.permissions@oup.com.
FAU - Suzuki, Yuji
AU  - Suzuki Y
AD  - Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka, 020-8550 Japan.
FAU - Ishiyama, Keiki
AU  - Ishiyama K
AD  - Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka, 020-8550 Japan.
AD  - Graduate School of Agricultural Science, Tohoku University, 468-1 Aramaki-Aoba, 
      Aoba-ku, Sendai, 980-8572 Japan.
FAU - Sugawara, Misaki
AU  - Sugawara M
AD  - Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka, 020-8550 Japan.
FAU - Suzuki, Yuka
AU  - Suzuki Y
AD  - Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka, 020-8550 Japan.
FAU - Kondo, Eri
AU  - Kondo E
AD  - Graduate School of Agricultural Science, Tohoku University, 468-1 Aramaki-Aoba, 
      Aoba-ku, Sendai, 980-8572 Japan.
FAU - Takegahara-Tamakawa, Yuki
AU  - Takegahara-Tamakawa Y
AD  - Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka, 020-8550 Japan.
FAU - Yoon, Dong-Kyung
AU  - Yoon DK
AD  - Graduate School of Agricultural Science, Tohoku University, 468-1 Aramaki-Aoba, 
      Aoba-ku, Sendai, 980-8572 Japan.
FAU - Suganami, Mao
AU  - Suganami M
AD  - Graduate School of Agricultural Science, Tohoku University, 468-1 Aramaki-Aoba, 
      Aoba-ku, Sendai, 980-8572 Japan.
FAU - Wada, Shinya
AU  - Wada S
AD  - Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka, 020-8550 Japan.
AD  - Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodai, Nada-ku, 
      Kobe, 657-8501 Japan.
FAU - Miyake, Chikahiro
AU  - Miyake C
AD  - Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodai, Nada-ku, 
      Kobe, 657-8501 Japan.
FAU - Makino, Amane
AU  - Makino A
AD  - Graduate School of Agricultural Science, Tohoku University, 468-1 Aramaki-Aoba, 
      Aoba-ku, Sendai, 980-8572 Japan.
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Plant Cell Physiol
JT  - Plant & cell physiology
JID - 9430925
RN  - 1406-65-1 (Chlorophyll)
RN  - 142M471B3J (Carbon Dioxide)
RN  - 9035-46-5 (Cytochromes f)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
RN  - EC 4.1.1.39 (Ribulose-Bisphosphate Carboxylase)
SB  - IM
MH  - Carbon Dioxide/metabolism
MH  - Chlorophyll/metabolism
MH  - Chloroplasts/*enzymology/metabolism
MH  - Cytochromes f/metabolism
MH  - Gene Expression Regulation, Plant
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases/*metabolism/physiology
MH  - Oryza/enzymology/*metabolism/physiology
MH  - *Photosynthesis
MH  - Plant Leaves/metabolism
MH  - Ribulose-Bisphosphate Carboxylase/metabolism
OTO - NOTNLM
OT  - Biomass production
OT  - CO2 assimilation
OT  - Calvin-Benson cycle
OT  - Chloroplast glyceraldehyde-3-phosphate dehydrogenase
OT  - Elevated [CO2] condition
OT  - Rice (Oryza sativa)
EDAT- 2020/12/09 06:00
MHDA- 2021/06/11 06:00
CRDT- 2020/12/08 08:34
PHST- 2020/09/15 00:00 [received]
PHST- 2020/11/16 00:00 [accepted]
PHST- 2020/12/09 06:00 [pubmed]
PHST- 2021/06/11 06:00 [medline]
PHST- 2020/12/08 08:34 [entrez]
AID - 6026967 [pii]
AID - 10.1093/pcp/pcaa149 [doi]
PST - ppublish
SO  - Plant Cell Physiol. 2021 Mar 25;62(1):156-165. doi: 10.1093/pcp/pcaa149.