PMID- 33289773
OWN - NLM
STAT- MEDLINE
DCOM- 20210331
LR  - 20210331
IS  - 2050-7518 (Electronic)
IS  - 2050-750X (Linking)
VI  - 9
IP  - 2
DP  - 2021 Jan 21
TI  - Dual-enzymatically cross-linked gelatin hydrogel enhances neural differentiation 
      of human umbilical cord mesenchymal stem cells and functional recovery in 
      experimental murine spinal cord injury.
PG  - 440-452
LID - 10.1039/d0tb02033h [doi]
AB  - Recently, an advanced stem cell and tissue engineering approach has been 
      recognized as an emerging and fascinating strategy to promote neural repair in 
      spinal cord injury (SCI). Hydrogels can be properly engineered to encapsulate 
      cells, enhance cell viability and neural differentiation, and provide the 
      advantage of flexible adaptation to irregular defects. In this study, a 
      dual-enzymatically cross-linked gelatin hydrogel with hydrogen horseradish 
      peroxidase (HRP) and galactose oxidase (GalOx) was proposed to combine human 
      umbilical cord mesenchymal stem cells (hUC-MSCs) for facilitating nerve 
      regeneration post-SCI. In vitro, hUC-MSCs in this 3D gelatin hydrogel displayed 
      good viability, proliferation, and neuronal differentiation. To further evaluate 
      the neural regeneration effect of hUC-MSCs loaded into gelatin hydrogels in vivo, 
      a clinically-relevant and force-controlled contusion model of mouse spinal cords 
      was established. We found that implantation of a hydrogel loaded with hUC-MSCs 
      significantly promoted the motor function recovery evaluated by Basso Mouse Scale 
      (BMS) and footprint tests. Further histological analysis showed that the hydrogel 
      and hUC-MSC combined transplantation dramatically decreased inflammation, 
      inhibited apoptosis and promoted neurogenesis. Overall, implantation of this 
      dual-enzymatically cross-linked and MSC-laden 3D gelatin hydrogel is a promising 
      therapeutic strategy for SCI treatment.
FAU - Yao, Minghao
AU  - Yao M
AUID- ORCID: 0000-0002-2232-0991
AD  - School of Life Science, Zhengzhou University, 100 Science Road, Zhengzhou 450001, 
      P. R. China. guanfangxia@126.com yao453343550@126.com.
FAU - Li, Jinrui
AU  - Li J
AD  - School of Life Science, Zhengzhou University, 100 Science Road, Zhengzhou 450001, 
      P. R. China. guanfangxia@126.com yao453343550@126.com.
FAU - Zhang, Junni
AU  - Zhang J
AD  - School of Life Science, Zhengzhou University, 100 Science Road, Zhengzhou 450001, 
      P. R. China. guanfangxia@126.com yao453343550@126.com.
FAU - Ma, Shanshan
AU  - Ma S
AD  - School of Life Science, Zhengzhou University, 100 Science Road, Zhengzhou 450001, 
      P. R. China. guanfangxia@126.com yao453343550@126.com.
FAU - Wang, Luyu
AU  - Wang L
AD  - School of Life Science, Zhengzhou University, 100 Science Road, Zhengzhou 450001, 
      P. R. China. guanfangxia@126.com yao453343550@126.com.
FAU - Gao, Feng
AU  - Gao F
AD  - School of Life Science, Zhengzhou University, 100 Science Road, Zhengzhou 450001, 
      P. R. China. guanfangxia@126.com yao453343550@126.com.
FAU - Guan, Fangxia
AU  - Guan F
AD  - School of Life Science, Zhengzhou University, 100 Science Road, Zhengzhou 450001, 
      P. R. China. guanfangxia@126.com yao453343550@126.com and Institute of 
      Neuroscience, Zhengzhou University, Zhengzhou 450000, P. R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Mater Chem B
JT  - Journal of materials chemistry. B
JID - 101598493
RN  - 0 (Hydrogels)
SB  - IM
MH  - Animals
MH  - Humans
MH  - Hydrogels/*chemistry
MH  - Mesenchymal Stem Cells/cytology/*metabolism
MH  - Mice
MH  - Spinal Cord Injuries/*therapy
MH  - Umbilical Cord/cytology/metabolism
EDAT- 2020/12/09 06:00
MHDA- 2021/04/01 06:00
CRDT- 2020/12/08 12:32
PHST- 2020/12/09 06:00 [pubmed]
PHST- 2021/04/01 06:00 [medline]
PHST- 2020/12/08 12:32 [entrez]
AID - 10.1039/d0tb02033h [doi]
PST - ppublish
SO  - J Mater Chem B. 2021 Jan 21;9(2):440-452. doi: 10.1039/d0tb02033h.