PMID- 33291693
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201226
IS  - 2072-666X (Print)
IS  - 2072-666X (Electronic)
IS  - 2072-666X (Linking)
VI  - 11
IP  - 12
DP  - 2020 Dec 4
TI  - Portable Device for Quick Detection of Viable Bacteria in Water.
LID - 10.3390/mi11121079 [doi]
LID - 1079
AB  - (1) Background: Access to clean water is a very important factor for human life. 
      However, pathogenic microorganisms in drinking water often cause diseases, and 
      convenient/inexpensive testing methods are urgently needed. (2) Methods: The 
      reagent contains 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide 
      (MTT) and phenazine methosulfate (PMS) and can react with succinate dehydrogenase 
      within bacterial cell membranes to produce visible purple crystals. The 
      colorimetric change of the reagent after reaction can be measured by a sensor 
      (AS7262). (3) Results: Compared with traditional methods, our device is simple to 
      operate and can provide rapid (i.e., 5 min) semi-quantitative results regarding 
      the concentration of bacteria within a test sample. (4) Conclusions: This 
      easy-to-use device, which employs MTT-PMS reagents, can be regarded as a 
      potential and portable tool for rapid water quality determination.
FAU - Liao, Yu-Hsiang
AU  - Liao YH
AD  - Institute of Biomedical Engineering, National Tsing Hua University, Hsinchu 300, 
      Taiwan.
FAU - Muthuramalingam, Karthickraj
AU  - Muthuramalingam K
AD  - Electronic and Optoelectronic System Research Laboratories, Industrial Technology 
      Research Institute, Hsinchu 310, Taiwan.
FAU - Tung, Kuo-Hao
AU  - Tung KH
AD  - Institute of Biomedical Engineering, National Tsing Hua University, Hsinchu 300, 
      Taiwan.
FAU - Chuan, Ho-Hsien
AU  - Chuan HH
AD  - Department of Surgery, National Taiwan University Hospital, Chu-Tung Branch, 
      Hsinchu 300, Taiwan.
FAU - Liang, Ko-Yuan
AU  - Liang KY
AD  - Center for Environmental Toxin and Emerging-Contaminant Research, Cheng Shiu 
      University, Kaohsiung 833, Taiwan.
AD  - Super Micro Mass Research and Technology Center, Cheng Shiu University, Kaohsiung 
      833, Taiwan.
FAU - Hsu, Chen-Peng
AU  - Hsu CP
AD  - Electronic and Optoelectronic System Research Laboratories, Industrial Technology 
      Research Institute, Hsinchu 310, Taiwan.
FAU - Cheng, Chao-Min
AU  - Cheng CM
AD  - Institute of Biomedical Engineering, National Tsing Hua University, Hsinchu 300, 
      Taiwan.
LA  - eng
PT  - Journal Article
DEP - 20201204
PL  - Switzerland
TA  - Micromachines (Basel)
JT  - Micromachines
JID - 101640903
PMC - PMC7761948
OTO - NOTNLM
OT  - MTT
OT  - PMS
OT  - bacterial detection
OT  - colorimetry
OT  - portable device
COIS- The authors declare no conflict of interest.
EDAT- 2020/12/10 06:00
MHDA- 2020/12/10 06:01
PMCR- 2020/12/04
CRDT- 2020/12/09 01:02
PHST- 2020/10/26 00:00 [received]
PHST- 2020/11/30 00:00 [revised]
PHST- 2020/12/03 00:00 [accepted]
PHST- 2020/12/09 01:02 [entrez]
PHST- 2020/12/10 06:00 [pubmed]
PHST- 2020/12/10 06:01 [medline]
PHST- 2020/12/04 00:00 [pmc-release]
AID - mi11121079 [pii]
AID - micromachines-11-01079 [pii]
AID - 10.3390/mi11121079 [doi]
PST - epublish
SO  - Micromachines (Basel). 2020 Dec 4;11(12):1079. doi: 10.3390/mi11121079.