PMID- 33292246 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20201212 IS - 1475-2867 (Print) IS - 1475-2867 (Electronic) IS - 1475-2867 (Linking) VI - 20 IP - 1 DP - 2020 Nov 26 TI - SNHG17 promotes the proliferation and migration of colorectal adenocarcinoma cells by modulating CXCL12-mediated angiogenesis. PG - 566 LID - 10.1186/s12935-020-01621-0 [doi] LID - 566 AB - BACKGROUND: Colorectal adenocarcinoma (CRA) is one of the leading causes of cancer-related deaths in the world. Long non-coding RNAs (lncRNAs) have been implicated to be effective regulators in the disease course of human cancers, including CRA. Small nucleolar RNA host gene 17 (SNHG17) belongs to lncRNAs, and it has been reported in breast cancer and gastric cancer. However, the function of SNHG17 and its mechanism in CRA progression remain largely unknown. In this study, we attended to shedding some light on the role of SNHG17 in CRA. METHODS: RT-qPCR was used to assess SNHG17 expression in CRA cells. CCK-8 assay, colony formation and transwell assay were carried out to detect the regulatory effect of SNHG17 silencing on CRA cell proliferation and migration. The angiogenesis of SNHG7-downregulated CRA cells was analyzed by tube formation assay. Mechanism experiments were conducted to identify the interaction between miR-23a-3p and SNHG17 or C-X-C motif chemokine ligand 12 (CXCL12). RESULTS: SNHG17 possessed with high expression in CRA cells. Knockdown of SNHG17 caused the inhibition on CRA cell proliferation and migration. SNHG17 promoted CRA cell proliferation and migration by sponging miR-23a-3p to upregulate CXCL12. CONCLUSION: SNHG17 promotes the proliferation and migration of CRA cells by inhibiting miR-23a-3p to modulate CXCL12-mediated angiogenesis. FAU - Liu, Yang AU - Liu Y AUID- ORCID: 0000-0002-4439-9119 AD - Department of Science and Education, The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, No. 71 Bao Shan North Road, Yunyan District, Guiyang, 550001, Guizhou, China. rhov66@163.com. AD - National & Guizhou Joint Engineering Laboratory for Cell Engineering and Biomedicine Technique, Guizhou Medical University, Guiyang, 550004, Guizhou, China. rhov66@163.com. FAU - Li, Qinshan AU - Li Q AD - National & Guizhou Joint Engineering Laboratory for Cell Engineering and Biomedicine Technique, Guizhou Medical University, Guiyang, 550004, Guizhou, China. FAU - Tang, Dongxin AU - Tang D AD - Department of Science and Education, The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, No. 71 Bao Shan North Road, Yunyan District, Guiyang, 550001, Guizhou, China. FAU - Li, Mengxing AU - Li M AD - Department of Hematology, Affiliated Hospital of Guizhou Medical University, No. 28 Guiyi Street, Yunyan District, Guiyang, 550004, Guizhou, China. FAU - Zhao, Peng AU - Zhao P AD - Department of Hematology, Affiliated Hospital of Guizhou Medical University, No. 28 Guiyi Street, Yunyan District, Guiyang, 550004, Guizhou, China. FAU - Yang, Wenxiu AU - Yang W AD - Department of Hematology, Affiliated Hospital of Guizhou Medical University, No. 28 Guiyi Street, Yunyan District, Guiyang, 550004, Guizhou, China. FAU - Shu, Liping AU - Shu L AD - National & Guizhou Joint Engineering Laboratory for Cell Engineering and Biomedicine Technique, Guizhou Medical University, Guiyang, 550004, Guizhou, China. FAU - Wang, Jishi AU - Wang J AD - Department of Hematology, Affiliated Hospital of Guizhou Medical University, No. 28 Guiyi Street, Yunyan District, Guiyang, 550004, Guizhou, China. FAU - He, Zhixu AU - He Z AD - National & Guizhou Joint Engineering Laboratory for Cell Engineering and Biomedicine Technique, Guizhou Medical University, Guiyang, 550004, Guizhou, China. AD - Department of Pediatrics, Affiliated Hospital of Zunyi Medical University, Zunyi, 563006, Guizhou, China. FAU - Li, Yanju AU - Li Y AD - Department of Hematology, Affiliated Hospital of Guizhou Medical University, No. 28 Guiyi Street, Yunyan District, Guiyang, 550004, Guizhou, China. nrad20@163.com. FAU - Wang, Feiqing AU - Wang F AD - Department of Science and Education, The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, No. 71 Bao Shan North Road, Yunyan District, Guiyang, 550001, Guizhou, China. ikjo66@163.com. LA - eng GR - No.2018M640938/China Postdoctoral Science Foundation/ GR - 43XB3794XB/China Postdoctoral Science Foundation/ GR - 31660326/the National Natural Science Foundation of China/ GR - 81871313/the National Natural Science Foundation of China/ GR - No.[2016]1019/Project Natural Science Foundation of Guizhou Province/ GR - [2015]2003/Project Natural Science Foundation of Guizhou Province/ GR - [2017]GZ66168/Project Natural Science Foundation of Guizhou Province/ GR - [2017]5735/Project Natural Science Foundation of Guizhou Province/ GR - [2018]2759/Project Natural Science Foundation of Guizhou Province/ GR - [2018]1015)/Project Natural Science Foundation of Guizhou Province/ GR - No.[2016]074/the Top Talent Foundation of the Department of Education of Guizhou Province/ GR - No.2017 PT31042/the Non-profit Central Research Institute Fund of Chinese Academy of Medical Sciences/ GR - 2018 PT31048/the Non-profit Central Research Institute Fund of Chinese Academy of Medical Sciences/ GR - No.[2019]9-2-2/Project Natural Science Foundation of Guiyang City/ GR - [2019]9-2-22/Project Natural Science Foundation of Guiyang City/ GR - [2019]9-2-34/Project Natural Science Foundation of Guiyang City/ GR - [2019]9-2-45/Project Natural Science Foundation of Guiyang City/ GR - No.GYZYYFY-BS-2018[12]/Doctoral Foundation of the First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine/ GR - NO.gzwjkj2017-1-046/Project Foundation of Guizhou Health Committee/ GR - NO.QZYY-2016-020/Project Foundation of Guizhou Administration of Traditional Chinese Medicine/ PT - Journal Article DEP - 20201126 PL - England TA - Cancer Cell Int JT - Cancer cell international JID - 101139795 PMC - PMC7690009 OTO - NOTNLM OT - Angiogenesis OT - CXCL12 OT - Colorectal adenocarcinoma OT - SNHG17 OT - miR-23a-3p COIS- The authors declare that there are no competing interests in this study. EDAT- 2020/12/10 06:00 MHDA- 2020/12/10 06:01 PMCR- 2020/11/26 CRDT- 2020/12/09 05:42 PHST- 2020/04/28 00:00 [received] PHST- 2020/10/26 00:00 [accepted] PHST- 2020/12/09 05:42 [entrez] PHST- 2020/12/10 06:00 [pubmed] PHST- 2020/12/10 06:01 [medline] PHST- 2020/11/26 00:00 [pmc-release] AID - 10.1186/s12935-020-01621-0 [pii] AID - 1621 [pii] AID - 10.1186/s12935-020-01621-0 [doi] PST - epublish SO - Cancer Cell Int. 2020 Nov 26;20(1):566. doi: 10.1186/s12935-020-01621-0.