PMID- 33293960
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201210
IS  - 1687-8760 (Print)
IS  - 1687-8779 (Electronic)
IS  - 1687-8760 (Linking)
VI  - 2020
DP  - 2020
TI  - A Validated Method for the Simultaneous Determination of Methamphetamine and 
      3,4-Methylenedioxy-N-methamphetamine in Blood-Based Liquid Chromatography-Tandem 
      Mass Spectrometry.
PG  - 8862679
LID - 10.1155/2020/8862679 [doi]
LID - 8862679
AB  - A liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method has been 
      validated for the simultaneous determination of methamphetamine (MA) and 
      3,4-methylenedioxy-N-methamphetamine (MDMA) in the blood sample. Under the 
      optimal experimental conditions, the concentration of MA can be determined in the 
      range from 1 microg/L to 5000 microg/L with the method detection limit (MDL) of 
      0.31 microg/L. The range from 0.5 to 500 microg/L is observed for the determination of 
      MDMA with the MDL down to 0.25 microg/L. The practical applicability of the method is 
      performed with the recovery ranging from 85.3% to 94% for MA and from 86.9% to 
      95.5% for MDMA. At the different concentrations of drugs, the relative standard 
      deviations (RSD) for both MA and MDMA are lower than 5.7%. The method was applied 
      to analyse 1995 blood samples that had been collected from the Forensic Medicine 
      Centre of Ho Chi Minh City. The results showed 1.75% positive with MA and 0.25% 
      positive with MDMA. These two drugs take 10% of the total drugs positive samples. 
      By using deuterium-labelled methamphetamine-d5 and 
      3,4-methylenedioxy-N-methamphetamine-d5 as the internal standards in the 
      determination and the use of MS/MS in multiple reaction monitoring mode signal 
      readout, the method exhibits robustness specificity and can be applied in 
      simultaneous determination of MA and MDMA in blood with high selectivity and 
      sensitivity.
CI  - Copyright (c) 2020 Dinh-Vu Le et al.
FAU - Le, Dinh-Vu
AU  - Le DV
AUID- ORCID: 0000-0003-2193-6193
AD  - Faculty of Chemical Engineering, Industrial University of Ho Chi Minh City, 12 
      Nguyen Van Bao Str. Go Vap, Ho Chi Minh City 70000, Vietnam.
FAU - Nguyen, Trong-Tuan
AU  - Nguyen TT
AD  - Faculty of Chemical Engineering, Industrial University of Ho Chi Minh City, 12 
      Nguyen Van Bao Str. Go Vap, Ho Chi Minh City 70000, Vietnam.
AD  - Forensic Medicine Centre of Ho Chi Minh City, 336 Tran Phu. District 5, Ho Chi 
      Minh City 70000, Vietnam.
FAU - Nguyen, Van-Trong
AU  - Nguyen VT
AD  - Faculty of Chemical Engineering, Industrial University of Ho Chi Minh City, 12 
      Nguyen Van Bao Str. Go Vap, Ho Chi Minh City 70000, Vietnam.
LA  - eng
PT  - Journal Article
DEP - 20201126
PL  - Egypt
TA  - Int J Anal Chem
JT  - International journal of analytical chemistry
JID - 101519424
PMC - PMC7714609
COIS- The authors report no conflicts of interest in this work.
EDAT- 2020/12/10 06:00
MHDA- 2020/12/10 06:01
PMCR- 2020/11/26
CRDT- 2020/12/09 06:13
PHST- 2020/07/08 00:00 [received]
PHST- 2020/09/08 00:00 [revised]
PHST- 2020/11/12 00:00 [accepted]
PHST- 2020/12/09 06:13 [entrez]
PHST- 2020/12/10 06:00 [pubmed]
PHST- 2020/12/10 06:01 [medline]
PHST- 2020/11/26 00:00 [pmc-release]
AID - 10.1155/2020/8862679 [doi]
PST - epublish
SO  - Int J Anal Chem. 2020 Nov 26;2020:8862679. doi: 10.1155/2020/8862679. eCollection 
      2020.