PMID- 33294264
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210505
IS  - 2156-6976 (Print)
IS  - 2156-6976 (Electronic)
IS  - 2156-6976 (Linking)
VI  - 10
IP  - 11
DP  - 2020
TI  - Circ-EEF2 facilitated autophagy via interaction with mir-6881-3p and ANXA2 in 
      EOC.
PG  - 3737-3751
AB  - Circular RNAs, a special class of non-coding RNA with closed circular structure, 
      have been increasingly proven to be involved in the progression of various 
      tumors. However, the biological functions of circular RNAs in epithelial ovarian 
      cancer (EOC) tissues remain a mystery. In this study, we detected the function of 
      circEEF2 (has-circ-0048559) in EOC tissues. Firstly, the basic characteristics 
      including closed circular structure and spliced mature sequence length of 
      circEEF2 were confirmed. The location and expression in EOC tissues was detected 
      by fluorescence in situ hybridization (FISH). The regulatory effect of circEEF2 
      on autophagy, proliferation, and invasion were investigated in SKOV3 and A2780 
      cells. The relationship between circEEF2 and mir-6881-3p was confirmed using 
      dual-luciferase reporter gene assay. The binding of circEEF2 with ANXA2 was 
      confirmed using RNA-pulldown assay and MALDI-TOF-MS. We found that the expression 
      level of circEEF2 was higher in EOC tissue than in normal tissue. CircEEF2 
      promoted autophagy, proliferation, and invasion. CircEEF2-regulated EOC 
      proliferation and invasion are closely related to the occurrence of autophagy. 
      Mechanistically, circEEF2 harbor miR-6881-3p to upregulate the latter's targets 
      ATG5 and ATG7. Moreover, circEEF2 could directly bind with ANXA2 to inhibit the 
      expression of p-mTOR. In conclusion, findings of the current study illustrate 
      that circEEF2 promoted autophagy, proliferation, and invasion of EOC by 
      interacting with miR-6881-3p and ANXA2.
CI  - AJCR Copyright (c) 2020.
FAU - Yong, Min
AU  - Yong M
AD  - Department of Obstetrics and Gynecology, Second Affiliated Hospital, Chongqing 
      Medical University Linjiang Road, No. 76, Chongqing 400010, China.
FAU - Hu, Jianguo
AU  - Hu J
AD  - Department of Obstetrics and Gynecology, Second Affiliated Hospital, Chongqing 
      Medical University Linjiang Road, No. 76, Chongqing 400010, China.
FAU - Zhu, Hongtao
AU  - Zhu H
AD  - Department of Obstetrics and Gynecology, Second Affiliated Hospital, Chongqing 
      Medical University Linjiang Road, No. 76, Chongqing 400010, China.
FAU - Jiang, Xinwei
AU  - Jiang X
AD  - Department of Obstetrics and Gynecology, Second Affiliated Hospital, Chongqing 
      Medical University Linjiang Road, No. 76, Chongqing 400010, China.
FAU - Gan, Xiaolin
AU  - Gan X
AD  - Department of Obstetrics and Gynecology, Second Affiliated Hospital, Chongqing 
      Medical University Linjiang Road, No. 76, Chongqing 400010, China.
FAU - Hu, Lina
AU  - Hu L
AD  - Department of Obstetrics and Gynecology, Second Affiliated Hospital, Chongqing 
      Medical University Linjiang Road, No. 76, Chongqing 400010, China.
AD  - Joint International Research Lab for Reproduction and Development, Ministry of 
      Education China.
LA  - eng
PT  - Journal Article
DEP - 20201101
PL  - United States
TA  - Am J Cancer Res
JT  - American journal of cancer research
JID - 101549944
EIN - Am J Cancer Res. 2021 Apr 15;11(4):1795-1799. PMID: 33948390
PMC - PMC7716148
OTO - NOTNLM
OT  - ANXA2
OT  - autophagy
OT  - circEEF2
OT  - epithelial ovarian cancer
OT  - miR-6881-3P
COIS- None.
EDAT- 2020/12/10 06:00
MHDA- 2020/12/10 06:01
PMCR- 2020/11/01
CRDT- 2020/12/09 06:14
PHST- 2020/07/21 00:00 [received]
PHST- 2020/10/13 00:00 [accepted]
PHST- 2020/12/09 06:14 [entrez]
PHST- 2020/12/10 06:00 [pubmed]
PHST- 2020/12/10 06:01 [medline]
PHST- 2020/11/01 00:00 [pmc-release]
PST - epublish
SO  - Am J Cancer Res. 2020 Nov 1;10(11):3737-3751. eCollection 2020.