PMID- 33295235
OWN - NLM
STAT- MEDLINE
DCOM- 20210930
LR  - 20210930
IS  - 1949-0984 (Electronic)
IS  - 1949-0976 (Print)
IS  - 1949-0976 (Linking)
VI  - 12
IP  - 1
DP  - 2020 Nov 9
TI  - Metabolic impact of persistent organic pollutants on gut microbiota.
PG  - 1-16
LID - 10.1080/19490976.2020.1848209 [doi]
LID - 1848209
AB  - Emerging evidence supports that exposure to persistent organic pollutants (POPs) 
      can impact the interaction between the gut microbiota and host. Recent efforts 
      have characterized the relationship between gut microbiota and environment 
      pollutants suggesting additional research is needed to understand potential new 
      avenues for toxicity. Here, we systematically examined the direct effects of POPs 
      including 2,3,7,8-tetrachlorodibenzofuran (TCDF), 
      2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), and polychlorinated biphenyls 
      (PCB-123 and PCB-156) on the microbiota using metatranscriptomics and NMR- and 
      mass spectrometry-based metabolomics combined with flow cytometry and growth rate 
      measurements (OD(600)). This study demonstrated that (1) POPs directly and 
      rapidly affect isolated cecal bacterial global metabolism that is associated with 
      significant decreases in microbial metabolic activity; (2) significant changes in 
      cecal bacterial gene expression related to tricarboxylic acid (TCA) cycle as well 
      as carbon metabolism, carbon fixation, pyruvate metabolism, and protein export 
      were observed following most POP exposure; (3) six individual bacterial species 
      show variation in lipid metabolism in response to POP exposure; and (4) PCB-153 
      (non-coplanar)has a greater impact on bacteria than PCB-126 (coplanar) at the 
      metabolic and transcriptional levels. These data provide new insights into the 
      direct role of POPs on gut microbiota and begins to establish possible microbial 
      toxicity endpoints which may help to inform risk assessment.
FAU - Tian, Yuan
AU  - Tian Y
AUID- ORCID: 0000-0001-6174-3359
AD  - Department of Veterinary and Biomedical Sciences, The Pennsylvania State 
      University, University Park , PA, USA.
FAU - Gui, Wei
AU  - Gui W
AD  - Department of Veterinary and Biomedical Sciences, The Pennsylvania State 
      University, University Park , PA, USA.
FAU - Rimal, Bipin
AU  - Rimal B
AUID- ORCID: 0000-0001-6528-5156
AD  - Department of Veterinary and Biomedical Sciences, The Pennsylvania State 
      University, University Park , PA, USA.
FAU - Koo, Imhoi
AU  - Koo I
AUID- ORCID: 0000-0002-5816-0627
AD  - Department of Veterinary and Biomedical Sciences, The Pennsylvania State 
      University, University Park , PA, USA.
FAU - Smith, Philip B
AU  - Smith PB
AD  - Huck Institutes of the Life Sciences, Huck Institutes of the Life Sciences, The 
      Pennsylvania State University, University Park , PA, USA.
FAU - Nichols, Robert G
AU  - Nichols RG
AD  - Department of Veterinary and Biomedical Sciences, The Pennsylvania State 
      University, University Park , PA, USA.
FAU - Cai, Jingwei
AU  - Cai J
AD  - Department of Veterinary and Biomedical Sciences, The Pennsylvania State 
      University, University Park , PA, USA.
FAU - Liu, Qing
AU  - Liu Q
AD  - Department of Veterinary and Biomedical Sciences, The Pennsylvania State 
      University, University Park , PA, USA.
FAU - Patterson, Andrew D
AU  - Patterson AD
AUID- ORCID: 0000-0003-2073-0070
AD  - Department of Veterinary and Biomedical Sciences, The Pennsylvania State 
      University, University Park , PA, USA.
LA  - eng
GR  - R01 ES028288/ES/NIEHS NIH HHS/United States
GR  - S10 OD021750/OD/NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Gut Microbes
JT  - Gut microbes
JID - 101495343
RN  - 0 (Benzofurans)
RN  - 0 (Polychlorinated Dibenzodioxins)
RN  - 38380-08-4 (2,3,3',4,4',5-hexachlorobiphenyl)
RN  - 7440-44-0 (Carbon)
RN  - 8558G7RUTR (Pyruvic Acid)
RN  - DFC2HB4I0K (Polychlorinated Biphenyls)
RN  - TSH69IA9XF (3,4,5,3',4'-pentachlorobiphenyl)
RN  - XZJ41GQI5D (2,3,7,8-tetrachlorodibenzofuran)
RN  - ZRU0C9E32O (2,4,5,2',4',5'-hexachlorobiphenyl)
SB  - IM
MH  - Animals
MH  - Bacteria/*metabolism
MH  - Benzofurans/toxicity
MH  - Carbon/metabolism
MH  - Cecum/drug effects/microbiology
MH  - Citric Acid Cycle/drug effects
MH  - Gastrointestinal Microbiome/*drug effects
MH  - Lipid Metabolism/drug effects
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Persistent Organic Pollutants/*toxicity
MH  - Polychlorinated Biphenyls/*toxicity
MH  - Polychlorinated Dibenzodioxins/*toxicity
MH  - Protein Transport/drug effects
MH  - Pyruvic Acid/metabolism
PMC - PMC7734116
OTO - NOTNLM
OT  - NMR
OT  - Persistent organic pollutants
OT  - flow cytometry
OT  - growth rate measurements
OT  - gut microbiota
OT  - mass spectrometry
OT  - metabolomics
OT  - metatranscriptomics
EDAT- 2020/12/10 06:00
MHDA- 2021/10/01 06:00
PMCR- 2020/12/09
CRDT- 2020/12/09 08:37
PHST- 2020/12/09 08:37 [entrez]
PHST- 2020/12/10 06:00 [pubmed]
PHST- 2021/10/01 06:00 [medline]
PHST- 2020/12/09 00:00 [pmc-release]
AID - 1848209 [pii]
AID - 10.1080/19490976.2020.1848209 [doi]
PST - ppublish
SO  - Gut Microbes. 2020 Nov 9;12(1):1-16. doi: 10.1080/19490976.2020.1848209.