PMID- 33297007
OWN - NLM
STAT- MEDLINE
DCOM- 20201221
LR  - 20201221
IS  - 1879-1298 (Electronic)
IS  - 0045-6535 (Linking)
VI  - 263
DP  - 2021 Jan
TI  - Comparative cytotoxicity studies of halophenolic disinfection byproducts using 
      human extended pluripotent stem cells.
PG  - 127899
LID - S0045-6535(20)32094-4 [pii]
LID - 10.1016/j.chemosphere.2020.127899 [doi]
AB  - 2,4,6-trichlorophenol (TCP), 2,4,6-tribromophenol (TBP) and 2,4,6-triiodophenol 
      (TIP) are a new class of halophenolic disinfection byproducts (DBPs) which have 
      been widely detected in drinking water. In recent years, their developmental 
      toxicity has got increasing public attention due to their potential toxic effects 
      on embryo development towards lower organisms. Nonetheless, the application of 
      human embryos for embryonic toxicologic studies is rendered by ethical and moral 
      considerations, as well as the technical barrier to sustaining normal development 
      beyond a few days. Human extended pluripotent stem (EPS) cells (novel 
      totipotent-like stem cells) represent a much more appropriate cellular model for 
      studying human embryo development. In this study, we utilized human EPS cells to 
      study the developmental toxicity of TCP, TBP and TIP, respectively. All three 
      halophenolic DBPs showed cytotoxicity against human EPS cells in an obvious 
      dose-dependent manner, among which TIP was the most cytotoxic one. Notably, the 
      expression of pluripotent genes in human EPS cells significantly declined after 
      2,4,6-trihalophenol exposure. Meanwhile, 2,4,6-trihalophenol exposure promoted 
      ectodermal differentiation of human EPS cells in an embryoid bodies (EBs) 
      differentiation assay, while both endodermal and mesodermal differentiation were 
      impaired. These results implied that phenolic halogenated DBPs have specific 
      effects on human embryo development even in the early stage of pregnancy. In 
      summary, we applied human EPS cells as a novel research model for human embryo 
      developmental toxicity study of environmental pollutants, and demonstrated the 
      toxicity of phenolic halogenated DBPs on early embryo development of human 
      beings.
CI  - Copyright (c) 2020 Elsevier Ltd. All rights reserved.
FAU - Liu, Yujie
AU  - Liu Y
AD  - State Key Laboratory of Pollution Control and Resource Reuse, School of the 
      Environment, Nanjing University, Nanjing, 210023, China.
FAU - Zhu, Dicong
AU  - Zhu D
AD  - State Key Laboratory of Reproductive Medicine, Nanjing Medical University, 
      Nanjing, 211166, China.
FAU - Zhao, Zhihua
AU  - Zhao Z
AD  - State Key Laboratory of Reproductive Medicine, Nanjing Medical University, 
      Nanjing, 211166, China.
FAU - Zhou, Qing
AU  - Zhou Q
AD  - State Key Laboratory of Pollution Control and Resource Reuse, School of the 
      Environment, Nanjing University, Nanjing, 210023, China.
FAU - Pan, Yang
AU  - Pan Y
AD  - State Key Laboratory of Pollution Control and Resource Reuse, School of the 
      Environment, Nanjing University, Nanjing, 210023, China.
FAU - Shi, Wei
AU  - Shi W
AD  - State Key Laboratory of Pollution Control and Resource Reuse, School of the 
      Environment, Nanjing University, Nanjing, 210023, China.
FAU - Qiu, Jingfan
AU  - Qiu J
AD  - Key Laboratory of Pathogen Biology of Jiangsu Province, Department of Pathogen 
      Biology, Nanjing Medical University, Nanjing, 211166, China. Electronic address: 
      qiujingfan@njmu.edu.cn.
FAU - Yang, Yang
AU  - Yang Y
AD  - State Key Laboratory of Reproductive Medicine, Nanjing Medical University, 
      Nanjing, 211166, China. Electronic address: yangyang11@njmu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20200815
PL  - England
TA  - Chemosphere
JT  - Chemosphere
JID - 0320657
RN  - 0 (Disinfectants)
RN  - 0 (Drinking Water)
RN  - 0 (Water Pollutants, Chemical)
SB  - IM
MH  - *Disinfectants
MH  - Disinfection
MH  - *Drinking Water
MH  - Halogenation
MH  - Humans
MH  - *Pluripotent Stem Cells/chemistry
MH  - *Water Pollutants, Chemical/analysis/toxicity
MH  - *Water Purification
OTO - NOTNLM
OT  - Developmental toxicity
OT  - Differentiation potency
OT  - Halophenolic disinfection byproducts
OT  - Human extended pluripotent stem cells
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2020/12/11 06:00
MHDA- 2020/12/22 06:00
CRDT- 2020/12/10 01:01
PHST- 2020/05/19 00:00 [received]
PHST- 2020/07/30 00:00 [revised]
PHST- 2020/08/01 00:00 [accepted]
PHST- 2020/12/10 01:01 [entrez]
PHST- 2020/12/11 06:00 [pubmed]
PHST- 2020/12/22 06:00 [medline]
AID - S0045-6535(20)32094-4 [pii]
AID - 10.1016/j.chemosphere.2020.127899 [doi]
PST - ppublish
SO  - Chemosphere. 2021 Jan;263:127899. doi: 10.1016/j.chemosphere.2020.127899. Epub 
      2020 Aug 15.