PMID- 33297397
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201226
IS  - 2072-6694 (Print)
IS  - 2072-6694 (Electronic)
IS  - 2072-6694 (Linking)
VI  - 12
IP  - 12
DP  - 2020 Dec 7
TI  - Mi-RNA-888-5p Is Involved in S-Adenosylmethionine Antitumor Effects in Laryngeal 
      Squamous Cancer Cells.
LID - 10.3390/cancers12123665 [doi]
LID - 3665
AB  - (1) Purpose: The methyl donor S-Adenosylmethionine (AdoMet) has been widely 
      explored as a therapeutic compound, and its application-alone or in combination 
      with other molecules-is emerging as a potential effective strategy for the 
      treatment and chemoprevention of tumours. In this study, we investigated the 
      antitumor activity of AdoMet in Laryngeal Squamous Cell Carcinoma (LSCC), 
      exploring the underlying mechanisms. (2) Results: We demonstrated that AdoMet 
      induced ROS generation and triggered autophagy with a consistent increase in 
      LC3B-II autophagy-marker in JHU-SCC-011 and HNO210 LSCC cells. AdoMet induced 
      ER-stress and activated UPR signaling through the upregulation of the spliced 
      form of XBP1 and CHOP. To gain new insights into the molecular mechanisms 
      underlying the antitumor activity of AdoMet, we evaluated the regulation of miRNA 
      expression profile and we found a downregulation of miR-888-5p. We transfected 
      LSCC cells with miR-888-5p inhibitor and exposed the cells to AdoMet for 48 and 
      72 h. The combination of AdoMet with miR-888-5p inhibitor synergistically induced 
      both apoptosis and inhibited cell migration paralleled by the up-regulation of 
      MYCBP and CDH1 genes and of their targets. (3) Conclusion: Overall, these data 
      highlighted that epigenetic reprogramming of miRNAs by AdoMet play an important 
      role in inhibiting apoptosis and migration in LSCC cell lines.
FAU - Pagano, Martina
AU  - Pagano M
AD  - Department of Precision Medicine, University of Campania "Luigi Vanvitelli", Via 
      L. De Crecchio 7, 80138 Naples, Italy.
FAU - Mosca, Laura
AU  - Mosca L
AD  - Department of Precision Medicine, University of Campania "Luigi Vanvitelli", Via 
      L. De Crecchio 7, 80138 Naples, Italy.
FAU - Vitiello, Francesca
AU  - Vitiello F
AD  - Department of Precision Medicine, University of Campania "Luigi Vanvitelli", Via 
      L. De Crecchio 7, 80138 Naples, Italy.
FAU - Ilisso, Concetta Paola
AU  - Ilisso CP
AD  - Department of Precision Medicine, University of Campania "Luigi Vanvitelli", Via 
      L. De Crecchio 7, 80138 Naples, Italy.
FAU - Coppola, Alessandra
AU  - Coppola A
AD  - Department of Precision Medicine, University of Campania "Luigi Vanvitelli", Via 
      L. De Crecchio 7, 80138 Naples, Italy.
FAU - Borzacchiello, Luigi
AU  - Borzacchiello L
AD  - Department of Precision Medicine, University of Campania "Luigi Vanvitelli", Via 
      L. De Crecchio 7, 80138 Naples, Italy.
FAU - Mele, Luigi
AU  - Mele L
AD  - Department of Experimental Medicine, University of Campania "Luigi Vanvitelli", 
      80138 Naples, Italy.
FAU - Caruso, Francesca Pia
AU  - Caruso FP
AUID- ORCID: 0000-0002-2206-1459
AD  - Department of Electrical Engineering and Information Technology (DIETI), 
      University of Naples "Federico II", 80128 Naples, Italy.
AD  - Bioinformatics Lab, BIOGEM, 83031 Ariano Irpino, Italy.
FAU - Ceccarelli, Michele
AU  - Ceccarelli M
AD  - Department of Electrical Engineering and Information Technology (DIETI), 
      University of Naples "Federico II", 80128 Naples, Italy.
AD  - Bioinformatics Lab, BIOGEM, 83031 Ariano Irpino, Italy.
FAU - Caraglia, Michele
AU  - Caraglia M
AUID- ORCID: 0000-0003-2408-6091
AD  - Department of Precision Medicine, University of Campania "Luigi Vanvitelli", Via 
      L. De Crecchio 7, 80138 Naples, Italy.
AD  - Laboratory of Precision and Molecular Oncology, BIOGEM, 83031 Ariano Irpino, 
      Italy.
FAU - Cacciapuoti, Giovanna
AU  - Cacciapuoti G
AD  - Department of Precision Medicine, University of Campania "Luigi Vanvitelli", Via 
      L. De Crecchio 7, 80138 Naples, Italy.
FAU - Porcelli, Marina
AU  - Porcelli M
AUID- ORCID: 0000-0003-0192-1728
AD  - Department of Precision Medicine, University of Campania "Luigi Vanvitelli", Via 
      L. De Crecchio 7, 80138 Naples, Italy.
LA  - eng
PT  - Journal Article
DEP - 20201207
PL  - Switzerland
TA  - Cancers (Basel)
JT  - Cancers
JID - 101526829
PMC - PMC7762311
OTO - NOTNLM
OT  - ER-stress
OT  - S-Adenosylmethionine
OT  - apoptosis
OT  - autophagy
OT  - laryngeal squamous cancer cells
OT  - miR-888
OT  - microRNA regulation
COIS- The authors declare no competing interests.
EDAT- 2020/12/11 06:00
MHDA- 2020/12/11 06:01
PMCR- 2020/12/07
CRDT- 2020/12/10 01:03
PHST- 2020/11/19 00:00 [received]
PHST- 2020/12/02 00:00 [accepted]
PHST- 2020/12/10 01:03 [entrez]
PHST- 2020/12/11 06:00 [pubmed]
PHST- 2020/12/11 06:01 [medline]
PHST- 2020/12/07 00:00 [pmc-release]
AID - cancers12123665 [pii]
AID - cancers-12-03665 [pii]
AID - 10.3390/cancers12123665 [doi]
PST - epublish
SO  - Cancers (Basel). 2020 Dec 7;12(12):3665. doi: 10.3390/cancers12123665.