PMID- 33297427
OWN - NLM
STAT- MEDLINE
DCOM- 20210419
LR  - 20210419
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 25
IP  - 23
DP  - 2020 Dec 7
TI  - Euodia pasteuriana Methanol Extract Exerts Anti-Inflammatory Effects by Targeting 
      TAK1 in the AP-1 Signaling Pathway.
LID - 10.3390/molecules25235760 [doi]
LID - 5760
AB  - Euodia pasteuriana A. Chev. ex Guillaumin, also known as Melicope accedens 
      (Blume) T.G. Hartley, is a herbal medicinal plant native to Vietnam. Although 
      Euodia pasteuriana is used as a traditional medicine to treat a variety of 
      inflammatory diseases, the pharmacological mechanisms related to this plant are 
      unclear. This study aimed to investigate the anti-inflammatory effects of a 
      methanol extract of Euodia pasteuriana leaves (Ep-ME) on the production of 
      inflammatory mediators, the mRNA expression of proinflammatory genes, and 
      inflammatory signaling activities in macrophage cell lines. The results showed 
      that Ep-ME strongly suppressed the release of nitric oxide (NO) in RAW264.7 cells 
      induced with lipopolysaccharide (LPS), pam3CysSerLys4 (Pam3CSK), and 
      polyinosinic-polycytidylic acid (poly I:C) without cytotoxicity. A reverse 
      transcription-polymerase chain reaction further confirmed that Ep-ME suppressed 
      the expression of interleukin 6 (IL-6), matrix metalloproteinase-1 (MMP1), matrix 
      metalloproteinase-2 (MMP2), matrix metalloproteinase-3 (MMP3), tumor necrosis 
      factor-alpha (TNF-alpha), and matrix metalloproteinase-9 (MMP9) at the transcriptional 
      level and reduced the luciferase activities of activator protein 1 (AP-1) 
      reporter promoters. In addition, immunoblotting analyses of the whole lysate and 
      nuclear fraction, as well as overexpression assays demonstrated that Ep-ME 
      decreased the translocation of c-Jun and suppressed the activation of 
      transforming growth factor beta-activated kinase 1 (TAK1) in the AP-1 signaling 
      pathways. These results imply that Ep-ME could be developed as an 
      anti-inflammatory agent that targets TAK1 in the AP-1 signaling pathway.
FAU - Zhang, Jianmei
AU  - Zhang J
AUID- ORCID: 0000-0003-0817-4809
AD  - Department of Integrative Biotechnology, and Biomedical Institute for Convergence 
      at SKKU (BICS), Sungkyunkwan University, Suwon 16419, Korea.
FAU - Kim, Mi-Yeon
AU  - Kim MY
AD  - School of Systems Biomedical Science, Soongsil University, Seoul 06978, Korea.
FAU - Cho, Jae Youl
AU  - Cho JY
AUID- ORCID: 0000-0001-8141-9927
AD  - Department of Integrative Biotechnology, and Biomedical Institute for Convergence 
      at SKKU (BICS), Sungkyunkwan University, Suwon 16419, Korea.
LA  - eng
GR  - 2017R1A6A1A03015642/National Research Foundation of Korea/
GR  - 2016R1D1A1B01011371/National Research Foundation of Korea/
PT  - Journal Article
DEP - 20201207
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Plant Extracts)
RN  - 0 (Solvents)
RN  - 0 (Transcription Factor AP-1)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 2.7.11.25 (MAP Kinase Kinase Kinases)
RN  - EC 2.7.11.25 (MAP kinase kinase kinase 7)
RN  - Y4S76JWI15 (Methanol)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/*chemistry/*pharmacology
MH  - Evodia/*chemistry
MH  - HEK293 Cells
MH  - Humans
MH  - MAP Kinase Kinase Kinases/*metabolism
MH  - Methanol/chemistry
MH  - Mice
MH  - Nitric Oxide/metabolism
MH  - Plant Extracts/*chemistry/*pharmacology
MH  - RAW 264.7 Cells
MH  - Signal Transduction/*drug effects
MH  - Solvents/chemistry
MH  - Transcription Factor AP-1/*metabolism
PMC - PMC7730574
OTO - NOTNLM
OT  - AP-1
OT  - Euodia pasteuriana
OT  - TAK1
OT  - anti-inflammatory
COIS- The authors declare no conflict of interest.
EDAT- 2020/12/11 06:00
MHDA- 2021/04/20 06:00
PMCR- 2020/12/07
CRDT- 2020/12/10 01:03
PHST- 2020/11/06 00:00 [received]
PHST- 2020/11/29 00:00 [revised]
PHST- 2020/12/05 00:00 [accepted]
PHST- 2020/12/10 01:03 [entrez]
PHST- 2020/12/11 06:00 [pubmed]
PHST- 2021/04/20 06:00 [medline]
PHST- 2020/12/07 00:00 [pmc-release]
AID - molecules25235760 [pii]
AID - molecules-25-05760 [pii]
AID - 10.3390/molecules25235760 [doi]
PST - epublish
SO  - Molecules. 2020 Dec 7;25(23):5760. doi: 10.3390/molecules25235760.