PMID- 33301051
OWN - NLM
STAT- MEDLINE
DCOM- 20210525
LR  - 20231213
IS  - 1573-4978 (Electronic)
IS  - 0301-4851 (Print)
IS  - 0301-4851 (Linking)
VI  - 48
IP  - 1
DP  - 2021 Jan
TI  - Phenotypic and molecular screenings for determination of cassava mosaic disease 
      (CMD) status in farmers' fields in Ebonyi State, Nigeria.
PG  - 227-240
LID - 10.1007/s11033-020-06039-5 [doi]
AB  - African cassava mosaic virus (ACMV) and East African cassava mosaic virus (EACMV) 
      are among the major constraints to cassava productivity within tropical and 
      sub-tropical regions, including Ebonyi State, Nigeria. Thus, virus indexing has 
      become imperative to determine the status of cassava mosaic disease (CMD) in 
      Ebonyi State, to implement appropriate preventive and control strategies. 
      Seventy-eight cassava accessions obtained from different locations of Ebonyi 
      State were phenotypically scored, using scales 1-5 depending on CMD symptomatic 
      expressions, followed by multiplex-PCR and sequencing for validation. 11% of 
      cassava accessions cultivated in Afikpo were resistant (RE) to ACMV compared to 
      8% of moderately-resistant (MR) accessions in Izzi and 55% of tolerant (TO) ACMV 
      accessions in Ebonyi. 100% of cassava accessions in Onicha and 66% in Afikpo 
      South were susceptible (SU) and highly susceptible (HS) to ACMV, respectively. 
      With multiplex-PCR, 97.4% (ACMV) and 2.6% (EACMV) were positive. Dunn's multiple 
      comparison tests of CMD mean incidence demonstrated differences (P < 0.05), 
      except between RE and MR, and TO and MR. More transitions (A/G, C/T) compared to 
      transversions (A/T, G/T), were detected, with nonsynonymous mutations 
      (Leucine/Isoleucine; Valine/Isoleucine; Arginine/Lysine; Methionine/Isoleucine), 
      and good bit-scores (91.13-99.07% identites; e-values of 7.00e(-148)-0.00e(+00)). 
      Phylogeny resolved the sequences into five major groups. DNA sequencing validated 
      the detected ACMV and EACMV species. This study revealed variants of ACMV and low 
      adoption of RE and MR cassava accessions in the farmers' fields. The findings 
      will guide in getting disease-free and resistant varieties as planting materials 
      to significantly mitigate the CMD spread in Ebonyi State, Nigeria.
FAU - Igwe, David Okeh
AU  - Igwe DO
AUID- ORCID: 0000-0001-7560-4173
AD  - Department of Biotechnology, Ebonyi State University, Abakaliki, Ebonyi, Nigeria. 
      dave25dato@yahoo.com.
AD  - Boyce Thompson Institute for Plant Research, Ithaca, NY, 14853, USA. 
      dave25dato@yahoo.com.
FAU - Anyanwu, Chidinma Blessing
AU  - Anyanwu CB
AD  - Department of Biotechnology, Ebonyi State University, Abakaliki, Ebonyi, Nigeria.
FAU - Afiukwa, Celestine Azubuike
AU  - Afiukwa CA
AD  - Department of Biotechnology, Ebonyi State University, Abakaliki, Ebonyi, Nigeria.
FAU - Nnamani, Catherine Veronica
AU  - Nnamani CV
AD  - Plant Taxonomy and Conservation Biology Research Unit, Department of Applied 
      Biology, Presco Campus, Ebonyi State University, Abakaliki, Nigeria.
FAU - Nweke, Friday Nwalo
AU  - Nweke FN
AD  - Biology/Microbiology/Biotechnology, Faculty Science, Federal University, 
      Ndufu-Alike Ikwo (FUNAI), Ikwo, Ebonyi, Nigeria.
FAU - Ude, George Nkem
AU  - Ude GN
AD  - Department of Natural Sciences, Bowie State University, Bowie, USA.
FAU - Ubi, Benjamin Ewa
AU  - Ubi BE
AD  - Department of Biotechnology, Ebonyi State University, Abakaliki, Ebonyi, Nigeria.
LA  - eng
PT  - Journal Article
DEP - 20201210
PL  - Netherlands
TA  - Mol Biol Rep
JT  - Molecular biology reports
JID - 0403234
RN  - 0 (DNA, Viral)
RN  - African cassava mosaic virus
SB  - IM
MH  - Begomovirus/genetics/*pathogenicity
MH  - DNA, Viral/genetics
MH  - Manihot/growth & development/*virology
MH  - Nigeria
MH  - *Phylogeny
MH  - Plant Diseases/genetics/*virology
MH  - Sequence Analysis, DNA
MH  - Nicotiana/growth & development/virology
PMC - PMC7727096
OTO - NOTNLM
OT  - African cassava mosaic virus
OT  - Manihot esculenta
OT  - Multiplex-PCR
OT  - Phylogeny
OT  - Symptoms
COIS- The authors declare that they have no competing interests.
EDAT- 2020/12/11 06:00
MHDA- 2021/05/26 06:00
PMCR- 2020/12/10
CRDT- 2020/12/10 12:16
PHST- 2020/09/09 00:00 [received]
PHST- 2020/11/27 00:00 [accepted]
PHST- 2020/12/11 06:00 [pubmed]
PHST- 2021/05/26 06:00 [medline]
PHST- 2020/12/10 12:16 [entrez]
PHST- 2020/12/10 00:00 [pmc-release]
AID - 10.1007/s11033-020-06039-5 [pii]
AID - 6039 [pii]
AID - 10.1007/s11033-020-06039-5 [doi]
PST - ppublish
SO  - Mol Biol Rep. 2021 Jan;48(1):227-240. doi: 10.1007/s11033-020-06039-5. Epub 2020 
      Dec 10.