PMID- 33302170
OWN - NLM
STAT- MEDLINE
DCOM- 20210514
LR  - 20210514
IS  - 1878-0180 (Electronic)
IS  - 1878-0180 (Linking)
VI  - 114
DP  - 2021 Feb
TI  - Fabrication of tissue-engineered tympanic membrane patches using 3D-Printing 
      technology.
PG  - 104219
LID - S1751-6161(20)30759-1 [pii]
LID - 10.1016/j.jmbbm.2020.104219 [doi]
AB  - In recent years, scaffolds produced in 3D printing technology have become more 
      widespread tool due to providing more advantages than traditional methods in 
      tissue engineering applications. In this research, it was aimed to produce 
      patches for the treatment of tympanic membrane perforations which caused 
      significant hearing loss by using 3D printing method. Polylactic acid(PLA) 
      scaffolds with Chitosan(CS) and Sodium Alginate(SA) added in various ratios were 
      prepared for artificial eardrum patches. Different amounts of chitosan and sodium 
      alginate added to PLA increased the biocompatibility of the produced scaffolds. 
      The created patches were designed by mimicking the thickness of the natural 
      tympanic membrane thanks to the precision provided by the 3D printed method. The 
      produced scaffolds were analyzed separately for chemical, morphological, 
      mechanical and biocompatibility properties. Scanning electron microscope (SEM), 
      Fourier-transform infrared (FT-IR) spectroscopy was performed to observe the 
      surface morphology and chemical structure of the scaffolds. Mechanical, thermal 
      and physical properties, swelling and degradation behaviors were examined to 
      fully analyze whole characteristic features of the samples. Cell culture study 
      was also performed to demonstrate the biocompatibility properties of the 
      fabricated scaffolds with human adipose tissue-derived mesenchymal stem cells 
      (hAD-MSCs). 15 wt % PLA was selected as the control group and among all 
      concentrations of CS and SA, groups containing 3 wt% CS and 3 wt% SA showed 
      significantly superior and favorable features in printing quality. The research 
      continued with these two scaffolds (3 wt% CS, and 3 wt% SA), which showed 
      improved print quality when added to PLA. Overall, these results show that PLA/CS 
      and PLA/SA 3D printed artificial patches have the potential to tissue engineering 
      solutions to repair tympanic membrane perforation for people with hearing loss.
CI  - Copyright (c) 2020 Elsevier Ltd. All rights reserved.
FAU - Ilhan, Elif
AU  - Ilhan E
AD  - Center for Nanotechnology & Biomaterials Application and Research (NBUAM), 
      Marmara University, Turkey; Department of Bioengineering, Faculty of Engineering, 
      Marmara University, 38000, 34722, Turkey.
FAU - Ulag, Songul
AU  - Ulag S
AD  - Center for Nanotechnology & Biomaterials Application and Research (NBUAM), 
      Marmara University, Turkey; Department of Metallurgical and Materials 
      Engineering, Faculty of Technology, Marmara University, Turkey.
FAU - Sahin, Ali
AU  - Sahin A
AD  - Department of Biochemistry, School of Medicine / Genetic and Metabolic Diseases 
      Research and Investigation Center, Marmara University, Istanbul, Turkey.
FAU - Yilmaz, Betul Karademir
AU  - Yilmaz BK
AD  - Department of Biochemistry, School of Medicine / Genetic and Metabolic Diseases 
      Research and Investigation Center, Marmara University, Istanbul, Turkey.
FAU - Ekren, Nazmi
AU  - Ekren N
AD  - Center for Nanotechnology & Biomaterials Application and Research (NBUAM), 
      Marmara University, Turkey; Department of Electrical and Electronics Engineering, 
      Faculty of Technology, Marmara University, Turkey.
FAU - Kilic, Osman
AU  - Kilic O
AD  - Center for Nanotechnology & Biomaterials Application and Research (NBUAM), 
      Marmara University, Turkey; Department of Electrical and Electronics Engineering, 
      Faculty of Technology, Marmara University, Turkey.
FAU - Sengor, Mustafa
AU  - Sengor M
AD  - Center for Nanotechnology & Biomaterials Application and Research (NBUAM), 
      Marmara University, Turkey.
FAU - Kalaskar, Deepak M
AU  - Kalaskar DM
AD  - UCL Division of Surgery and Interventional Science, Royal National Orthopaedic 
      Hospital, Brockely Hill, HA7 4LP, UK.
FAU - Oktar, Faik Nuzhet
AU  - Oktar FN
AD  - Center for Nanotechnology & Biomaterials Application and Research (NBUAM), 
      Marmara University, Turkey; Department of Bioengineering, Faculty of Engineering, 
      Marmara University, 38000, 34722, Turkey.
FAU - Gunduz, Oguzhan
AU  - Gunduz O
AD  - Center for Nanotechnology & Biomaterials Application and Research (NBUAM), 
      Marmara University, Turkey; Department of Metallurgical and Materials 
      Engineering, Faculty of Technology, Marmara University, Turkey. Electronic 
      address: ucemogu@ucl.ac.uk.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201202
PL  - Netherlands
TA  - J Mech Behav Biomed Mater
JT  - Journal of the mechanical behavior of biomedical materials
JID - 101322406
SB  - IM
MH  - Humans
MH  - Printing, Three-Dimensional
MH  - Spectroscopy, Fourier Transform Infrared
MH  - *Tissue Engineering
MH  - *Tissue Scaffolds
MH  - Tympanic Membrane
OTO - NOTNLM
OT  - 3D printing
OT  - Artificial eardrum patch
OT  - Biomaterials
OT  - Tissue engineering
OT  - Tympanic membrane
EDAT- 2020/12/11 06:00
MHDA- 2021/05/15 06:00
CRDT- 2020/12/10 20:13
PHST- 2020/03/23 00:00 [received]
PHST- 2020/10/31 00:00 [revised]
PHST- 2020/11/20 00:00 [accepted]
PHST- 2020/12/11 06:00 [pubmed]
PHST- 2021/05/15 06:00 [medline]
PHST- 2020/12/10 20:13 [entrez]
AID - S1751-6161(20)30759-1 [pii]
AID - 10.1016/j.jmbbm.2020.104219 [doi]
PST - ppublish
SO  - J Mech Behav Biomed Mater. 2021 Feb;114:104219. doi: 10.1016/j.jmbbm.2020.104219. 
      Epub 2020 Dec 2.