PMID- 33302229
OWN - NLM
STAT- MEDLINE
DCOM- 20210514
LR  - 20210514
IS  - 2211-0356 (Electronic)
IS  - 2211-0348 (Linking)
VI  - 47
DP  - 2021 Jan
TI  - Polymorphisms in proinflammatory cytokines genes and susceptibility to Multiple 
      Sclerosis.
PG  - 102654
LID - S2211-0348(20)30728-8 [pii]
LID - 10.1016/j.msard.2020.102654 [doi]
AB  - BACKGROUND: Multiple sclerosis (MS) is a chronic inflammatory and 
      neurodegenerative disorder of the central nervous system (CNS). It is 
      immunologically induced in genetically susceptible individuals. Proinflammatory 
      cytokines play an important role as genetic polymorphisms in their genes might be 
      involved in the susceptibility and pathogenesis of MS. OBJECTIVE: In this study, 
      our goal was to analyze the association between the gene polymorphisms in 
      interleukin-16 (IL-16) (rs4072111 C/T), tumor necrosis factor-alpha (TNF-alpha) -308 G/A 
      (rs1800629 G/A) and IL-18 -607 C/A (rs1946518 C/A) and the susceptibility and 
      clinical features of MS in an Egyptian cohort. METHODS: We genotyped these 
      genetic polymorphisms in 150 subjects including 93 patients with MS and 57 
      unrelated healthy subjects. We employed polymerase chain reaction- restriction 
      fragment length polymorphism (PCR-RFLP) method for determining the IL-16 
      (rs4072111 C/T) and TNF-alpha -308 G/A (rs1800629 G/A) polymorphisms, and the allele- 
      specific polymerase chain reaction (AS-PCR) method for IL-18-607 C/A (rs1946518 
      C/A) polymorphism. RESULTS: The IL-16 (rs4072111 C/T) polymorphism was not 
      polymorphic in both MS patients and the healthy volunteers. For the TNF-alpha-308 G/A 
      (rs1800629 G/A) polymorphism, the mutant AA genotype and A allele are not 
      associated with the susceptibility of MS, however, associated with the severity 
      and disability progression of the disease. We observed a statistically 
      significant increase in the mean values of Expanded Disability Status Scale 
      (EDSS) and Multiple Sclerosis Severity Score (MSSS) in patients with AA genotype 
      and A allele compared with those of genotypes GG and GA, and the G allele, and 
      regression analysis confirmed that this polymorphism is a predictor of disease 
      disability using EDSS. For the IL-18 -607 C/A (rs1946518 C/A) polymorphism, the 
      frequency of mutant AA genotype and A allele showed significant differences 
      between the MS patients and healthy controls. CONCLUSION: The TNF-alpha-308 AA 
      genotype and A allele could be related to disability progression and severity of 
      MS and the IL-18-607 AA genotype A allele could be related to susceptibility of 
      the disease in the Egyptian cohort.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Bakr, Noha M
AU  - Bakr NM
AD  - Biochemistry Department, Genetic Engineering and Biotechnology Research Division, 
      National Research Centre (NRC), Giza, Egypt. Electronic address: 
      noni_mb@yahoo.com.
FAU - Hashim, Noha A
AU  - Hashim NA
AD  - Neurology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt. 
      Electronic address: Noha.ali@zu.edu.eg.
FAU - El-Baz, Hatim Alaa El-Din
AU  - El-Baz HAE
AD  - Biochemistry Department, Genetic Engineering and Biotechnology Research Division, 
      National Research Centre (NRC), Giza, Egypt. Electronic address: 
      helbaz78@yahoo.com.
FAU - Khalaf, Eman Mohammad
AU  - Khalaf EM
AD  - Department of Microbiology and Immunology, Faculty of Pharmacy, Damanhour 
      University, Egypt. Electronic address: eimanpharmacist@gmail.com.
FAU - Elharoun, Ahmed Shukry
AU  - Elharoun AS
AD  - Microbiology Department, Faculty of Medicine, Menoufiya University, Menoufiya, 
      Egypt. Electronic address: aelharoun@hotmail.com.
LA  - eng
PT  - Journal Article
DEP - 20201128
PL  - Netherlands
TA  - Mult Scler Relat Disord
JT  - Multiple sclerosis and related disorders
JID - 101580247
RN  - 0 (Cytokines)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Case-Control Studies
MH  - Cytokines/genetics
MH  - Egypt
MH  - Genetic Predisposition to Disease
MH  - Genotype
MH  - Humans
MH  - Interleukin-10
MH  - *Multiple Sclerosis/genetics
MH  - Polymorphism, Single Nucleotide/genetics
OTO - NOTNLM
OT  - Multiple sclerosis
OT  - Polymorphisms
OT  - Prognosis
OT  - Proinflammatory cytokines
OT  - Severity
EDAT- 2020/12/11 06:00
MHDA- 2021/05/15 06:00
CRDT- 2020/12/10 20:15
PHST- 2020/09/25 00:00 [received]
PHST- 2020/11/16 00:00 [revised]
PHST- 2020/11/23 00:00 [accepted]
PHST- 2020/12/11 06:00 [pubmed]
PHST- 2021/05/15 06:00 [medline]
PHST- 2020/12/10 20:15 [entrez]
AID - S2211-0348(20)30728-8 [pii]
AID - 10.1016/j.msard.2020.102654 [doi]
PST - ppublish
SO  - Mult Scler Relat Disord. 2021 Jan;47:102654. doi: 10.1016/j.msard.2020.102654. 
      Epub 2020 Nov 28.