PMID- 33304230
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240330
IS  - 1618-0240 (Print)
IS  - 1618-2863 (Electronic)
IS  - 1618-0240 (Linking)
VI  - 20
IP  - 12
DP  - 2020 Dec
TI  - Bcl-xL expression following articular cartilage injury and its effects on the 
      biological function of chondrocytes.
PG  - 571-579
LID - 10.1002/elsc.202000039 [doi]
AB  - This study aimed to investigate the expression of B-cell lymphoma-extra large 
      (Bcl-xL) in cartilage tissues following articular cartilage injury and to 
      determine its effects on the biological function of chondrocytes. A total of 25 
      necrotic cartilage tissue samples and 25 normal tissue samples were collected 
      from patients diagnosed with osteoarthritis at our hospital from December 2015 to 
      December 2018. The mRNA expression levels of Bcl-xL, caspase-3, and matrix 
      metalloproteinase-3 (MMP-3) in the normal and necrotic tissues were examined via 
      quantitative polymerase chain reaction, and their protein expression levels were 
      detected via western blotting. The expression levels of Bcl-xL, insulin-like 
      growth factor-1 (IGF-1), and bone morphogenetic protein (BMP) were significantly 
      lower but those of caspase-3, MMP-3, interleukin-1beta (IL-1beta), and chemokine-like 
      factor 1 (CKLF1) levels were markedly higher in necrotic cartilage tissues than 
      in normal tissues. Following cell transfection, the expression levels of Bcl-xL, 
      IGF-1, and BMP were remarkably higher but those of caspase-3, MMP-3, IL-1beta, and 
      CKLF1 were notably lower in the Si-Bcl-xL group than in the NC group. The 
      Si-Bcl-xL group showed significantly lower cell growth and noticeably higher 
      apoptosis rate than the NC group (normal control group). The expression of Bcl-xL 
      is reduced following articular cartilage injury, and this reduction promotes the 
      proliferation and inhibits the apoptosis of chondrocytes. Therefore, Bcl-xL could 
      serve as a relevant molecular target in the clinical practice of osteoarthritis 
      and other diseases causing cartilage damage.
CI  - (c) 2020 The Authors. Engineering in Life Sciences published by Wiley-VCH GmbH.
FAU - Pan, Zhengjun
AU  - Pan Z
AD  - Orthopedics Department The First Affiliated Hospital of Anhui Medical University 
      Hefei Anhui P. R. China.
AD  - Orthopedics Department The First People's Hospital of Hefei Hefei Anhui P. R. 
      China.
FAU - Yin, Hao
AU  - Yin H
AD  - Orthopedics Department The First People's Hospital of Hefei Hefei Anhui P. R. 
      China.
FAU - Wang, Shuangli
AU  - Wang S
AD  - Orthopedics Department The First People's Hospital of Hefei Hefei Anhui P. R. 
      China.
FAU - Xiong, Gaoxin
AU  - Xiong G
AD  - Orthopedics Department The First People's Hospital of Hefei Hefei Anhui P. R. 
      China.
FAU - Yin, Zongsheng
AU  - Yin Z
AD  - Orthopedics Department The First Affiliated Hospital of Anhui Medical University 
      Hefei Anhui P. R. China.
LA  - eng
PT  - Journal Article
DEP - 20201013
PL  - Germany
TA  - Eng Life Sci
JT  - Engineering in life sciences
JID - 101193313
PMC - PMC7708954
OTO - NOTNLM
OT  - BMP
OT  - Bcl-xL
OT  - CKLF1
OT  - IGF-1
OT  - IL-1beta
OT  - MMP-3
OT  - cartilage damage
OT  - caspase-3
OT  - osteoarthritis
COIS- The autors have declared no conflict of interest.
EDAT- 2020/12/12 06:00
MHDA- 2020/12/12 06:01
PMCR- 2020/10/13
CRDT- 2020/12/11 06:01
PHST- 2020/05/22 00:00 [received]
PHST- 2020/09/08 00:00 [revised]
PHST- 2020/09/14 00:00 [accepted]
PHST- 2020/12/11 06:01 [entrez]
PHST- 2020/12/12 06:00 [pubmed]
PHST- 2020/12/12 06:01 [medline]
PHST- 2020/10/13 00:00 [pmc-release]
AID - ELSC1343 [pii]
AID - 10.1002/elsc.202000039 [doi]
PST - epublish
SO  - Eng Life Sci. 2020 Oct 13;20(12):571-579. doi: 10.1002/elsc.202000039. 
      eCollection 2020 Dec.