PMID- 33306387
OWN - NLM
STAT- MEDLINE
DCOM- 20210402
LR  - 20210402
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 68
IP  - 51
DP  - 2020 Dec 23
TI  - Inhibitory Effects of Green Asparagus Extract, Especially Phospholipids, on 
      Allergic Responses in Vitro and in Vivo.
PG  - 15199-15207
LID - 10.1021/acs.jafc.0c05615 [doi]
AB  - Asparagus (Asparagus officinalis L.) is one of the widely consumed vegetables. To 
      investigate the mechanism underlying the anti-allergic responses of asparagus, we 
      extracted different fractions from asparagus and measured their inhibitory 
      effects on beta-hexosaminidase release in RBL-2H3 cells in vitro and an atopic 
      dermatitis NC/Nga mouse model in vivo. The lipid fractions from asparagus were 
      extracted with 50% ethanol, separated using chloroform by liquid-liquid phase 
      separation, and fractionated by solid-phase extraction. Among them, acetone 
      fraction (rich in glycolipid) and MeOH fraction (rich in phospholipid) markedly 
      inhibited beta-hexosaminidase release from RBL-2H3 cells. In NC/Nga mice treated 
      with picryl chloride, atopic dermatitis was alleviated following exposure to the 
      50% EtOH extract, acetone fraction, and methanol fraction. The inhibitory effects 
      of asparagus fractions in vivo were supported by the significant decrease in 
      serum immunoglobulin E (IgE) levels. The phospholipid fractions showed 
      significantly better inhibitory effects, and phosphatidic acid from this fraction 
      showed the best inhibitory effect on beta-hexosaminidase release. In mice challenged 
      with ovalbumin (OVA), oral administration of asparagus extract and its fractions 
      decreased the OVA-specific IgE level and total IgE, indicating that these effects 
      may be partly mediated through the downregulation of antigen-specific IgE 
      production. Taken together, the present study shows for the first time that 
      asparagus extract and its lipid fractions could potentially mitigate allergic 
      reactions by decreasing degranulation in granulocytes. Our study provides useful 
      information to develop nutraceuticals and functional foods fortified with 
      asparagus.
FAU - Iwamoto, Akira
AU  - Iwamoto A
AUID- ORCID: 0000-0001-6455-2494
AD  - Division of Food Industry, Industrial Technology Center of Saga, 114 Yaemizo, 
      Nabeshima-machi, Saga 829-0932, Japan.
FAU - Hamajima, Hiroshi
AU  - Hamajima H
AD  - Saga Food & Cosmetic Laboratory, Division of Research and Development Promotion, 
      Saga Regional Industry Support Center, 114 Yaemizo, Nabeshima-machi, Saga 
      829-0932, Japan.
FAU - Tsuge, Keisuke
AU  - Tsuge K
AD  - Division of Food Industry, Industrial Technology Center of Saga, 114 Yaemizo, 
      Nabeshima-machi, Saga 829-0932, Japan.
FAU - Tsuruta, Yumi
AU  - Tsuruta Y
AD  - Division of Food Industry, Industrial Technology Center of Saga, 114 Yaemizo, 
      Nabeshima-machi, Saga 829-0932, Japan.
FAU - Nagata, Yasuo
AU  - Nagata Y
AD  - Saga Food & Cosmetic Laboratory, Division of Research and Development Promotion, 
      Saga Regional Industry Support Center, 114 Yaemizo, Nabeshima-machi, Saga 
      829-0932, Japan.
AD  - Center for Industry, University and Government Cooperation, Nagasaki University, 
      1-14 Bunkyo-machi, Nagasaki 852-8521, Japan.
FAU - Yotsumoto, Hiroaki
AU  - Yotsumoto H
AD  - Department of Health and Nutrition Sciences, Nishikyushu University, 4490-9 
      Ozaki, Saga 842-8585, Japan.
FAU - Yanagita, Teruyoshi
AU  - Yanagita T
AD  - Saga Food & Cosmetic Laboratory, Division of Research and Development Promotion, 
      Saga Regional Industry Support Center, 114 Yaemizo, Nabeshima-machi, Saga 
      829-0932, Japan.
AD  - Department of Health and Nutrition Sciences, Nishikyushu University, 4490-9 
      Ozaki, Saga 842-8585, Japan.
AD  - Department of Applied Biochemistry and Food Science, Saga University, 1 Honjo, 
      Honjo-machi, Saga 840-8502, Japan.
LA  - eng
PT  - Journal Article
DEP - 20201211
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Anti-Allergic Agents)
RN  - 0 (Phospholipids)
RN  - 0 (Plant Extracts)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - EC 3.2.1.- (Hexosaminidases)
SB  - IM
MH  - Animals
MH  - Anti-Allergic Agents/*administration & dosage/chemistry/isolation & purification
MH  - Asparagus Plant/*chemistry
MH  - Dermatitis, Atopic/*drug therapy/*immunology
MH  - Female
MH  - Granulocytes/drug effects/immunology
MH  - Hexosaminidases/immunology
MH  - Humans
MH  - Immunoglobulin E/immunology
MH  - Mice, Inbred BALB C
MH  - Phospholipids/*administration & dosage/chemistry/isolation & purification
MH  - Plant Extracts/*administration & dosage/chemistry/isolation & purification
OTO - NOTNLM
OT  - anti-allergy
OT  - asparagus
OT  - degranulation
OT  - phospholipids
OT  - beta-hexosaminidase
EDAT- 2020/12/12 06:00
MHDA- 2021/04/07 06:00
CRDT- 2020/12/11 17:07
PHST- 2020/12/12 06:00 [pubmed]
PHST- 2021/04/07 06:00 [medline]
PHST- 2020/12/11 17:07 [entrez]
AID - 10.1021/acs.jafc.0c05615 [doi]
PST - ppublish
SO  - J Agric Food Chem. 2020 Dec 23;68(51):15199-15207. doi: 10.1021/acs.jafc.0c05615. 
      Epub 2020 Dec 11.