PMID- 33307057
OWN - NLM
STAT- MEDLINE
DCOM- 20210308
LR  - 20210308
IS  - 1872-7573 (Electronic)
IS  - 0378-8741 (Linking)
VI  - 268
DP  - 2021 Mar 25
TI  - Chemical composition of Prunus padus L. flower extract and its anti-inflammatory 
      activities in primary bone marrow-derived macrophages.
PG  - 113678
LID - S0378-8741(20)33566-2 [pii]
LID - 10.1016/j.jep.2020.113678 [doi]
AB  - ETHNOPHARMACOLOGICAL RELEVANCE: Prunus padus L. has been traditionally used in 
      European ethnomedicine as a treatment for internal and external purposes and is 
      mainly used to reduce inflammation, pain and fever. The activities of P. padus 
      flower extracts are not well characterized, and additional experimental studies 
      at the molecular level are needed to confirm the ethnobotanical findings. AIM OF 
      THE STUDY: To assess the potential of P. padus flower extract (PPFE) as a source 
      of bioactive compounds through the characterization of its chemical composition 
      and antioxidant, anti-collagenase, and anti-inflammatory activities. MATERIALS 
      AND METHODS: The ethanolic extract (1:10 w/v in ethanol solution) from P. padus 
      flowers was subjected to phytochemical analysis and evaluation of the 
      2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity. 
      Anti-collagenase activity was determined using a spectrophotometric method in 
      vitro. The effect of PPFE on inflammation was evaluated by measuring specific 
      markers using flow cytometry and assessing pro-inflammatory cytokine (IL-6) 
      release by bone marrow-derived macrophages (BMDMs) ex vivo. RESULTS: The major 
      components of the ethanolic extract of P. padus flowers were quercetin 
      diglycosides, chlorogenic acid and N',N''-dicaffeoyl,N'''-coumaroyl spermidine. The 
      total phenolic content of PPFE was 85.19 mg GAE/g extract, and the EC(50) value 
      in the DPPH assay was 0.55 mg/ml. PPFE exhibited the ability to inhibit 
      collagenase activity in a dose-dependent manner. Preincubation of BMDMs with PPFE 
      reduced the population of M1 (pro-inflammatory) and increased the population of 
      M2 (anti-inflammatory) macrophages. Furthermore, PPFE decreased pro-inflammatory 
      cytokine IL-6 release from BMDMs. CONCLUSIONS: PPFE is a rich source of bioactive 
      compounds and possesses considerable anti-inflammatory properties, supporting its 
      use in ethnomedicine for the reduction of inflammatory processes.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Sile, Inga
AU  - Sile I
AD  - Latvian Institute of Organic Synthesis, 21 Aizkraukles Str., LV-1006, Riga, 
      Latvia; Department of Dosage Form Technology, Riga Stradins University, 16 
      Dzirciema Str, Riga, LV-1007, Latvia. Electronic address: inga.sile@farm.osi.lv.
FAU - Videja, Melita
AU  - Videja M
AD  - Latvian Institute of Organic Synthesis, 21 Aizkraukles Str., LV-1006, Riga, 
      Latvia; Department of Pharmaceutical Chemistry, Riga Stradins University, 16 
      Dzirciema Str, Riga, LV-1007, Latvia. Electronic address: 
      melita.videja@farm.osi.lv.
FAU - Makrecka-Kuka, Marina
AU  - Makrecka-Kuka M
AD  - Latvian Institute of Organic Synthesis, 21 Aizkraukles Str., LV-1006, Riga, 
      Latvia. Electronic address: makrecka@farm.osi.lv.
FAU - Tirzite, Dace
AU  - Tirzite D
AD  - Latvian Institute of Organic Synthesis, 21 Aizkraukles Str., LV-1006, Riga, 
      Latvia. Electronic address: tirzite@latnet.lv.
FAU - Pajuste, Karlis
AU  - Pajuste K
AD  - Latvian Institute of Organic Synthesis, 21 Aizkraukles Str., LV-1006, Riga, 
      Latvia. Electronic address: kpajuste@osi.lv.
FAU - Shubin, Kirill
AU  - Shubin K
AD  - Latvian Institute of Organic Synthesis, 21 Aizkraukles Str., LV-1006, Riga, 
      Latvia. Electronic address: kir101@osi.lv.
FAU - Krizhanovska, Valerija
AU  - Krizhanovska V
AD  - Latvian Institute of Organic Synthesis, 21 Aizkraukles Str., LV-1006, Riga, 
      Latvia. Electronic address: valerija@osi.lv.
FAU - Grinberga, Solveiga
AU  - Grinberga S
AD  - Latvian Institute of Organic Synthesis, 21 Aizkraukles Str., LV-1006, Riga, 
      Latvia. Electronic address: solveiga@osi.lv.
FAU - Pugovics, Osvalds
AU  - Pugovics O
AD  - Latvian Institute of Organic Synthesis, 21 Aizkraukles Str., LV-1006, Riga, 
      Latvia. Electronic address: osvalds@osi.lv.
FAU - Dambrova, Maija
AU  - Dambrova M
AD  - Latvian Institute of Organic Synthesis, 21 Aizkraukles Str., LV-1006, Riga, 
      Latvia; Department of Pharmaceutical Chemistry, Riga Stradins University, 16 
      Dzirciema Str, Riga, LV-1007, Latvia. Electronic address: 
      maija.dambrova@farm.osi.lv.
LA  - eng
PT  - Journal Article
DEP - 20201208
PL  - Ireland
TA  - J Ethnopharmacol
JT  - Journal of ethnopharmacology
JID - 7903310
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Plant Extracts)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/isolation & purification/*pharmacology
MH  - Dose-Response Relationship, Drug
MH  - *Flowers
MH  - Macrophages/*drug effects/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Plant Extracts/isolation & purification/*pharmacology
MH  - *Prunus
OTO - NOTNLM
OT  - Anti-inflammatory activity
OT  - Ethanolic extract
OT  - GC-MS
OT  - LC-MS
OT  - Prunus padus
EDAT- 2020/12/12 06:00
MHDA- 2021/03/09 06:00
CRDT- 2020/12/11 20:09
PHST- 2020/10/20 00:00 [received]
PHST- 2020/12/02 00:00 [revised]
PHST- 2020/12/04 00:00 [accepted]
PHST- 2020/12/12 06:00 [pubmed]
PHST- 2021/03/09 06:00 [medline]
PHST- 2020/12/11 20:09 [entrez]
AID - S0378-8741(20)33566-2 [pii]
AID - 10.1016/j.jep.2020.113678 [doi]
PST - ppublish
SO  - J Ethnopharmacol. 2021 Mar 25;268:113678. doi: 10.1016/j.jep.2020.113678. Epub 
      2020 Dec 8.