PMID- 33309840 OWN - NLM STAT- MEDLINE DCOM- 20211029 LR - 20211029 IS - 1872-9711 (Electronic) IS - 0161-813X (Print) IS - 0161-813X (Linking) VI - 82 DP - 2021 Jan TI - Exposure to bisphenol A differentially impacts neurodevelopment and behavior in Drosophila melanogaster from distinct genetic backgrounds. PG - 146-157 LID - S0161-813X(20)30214-X [pii] LID - 10.1016/j.neuro.2020.12.007 [doi] AB - Bisphenol A (BPA) is a ubiquitous environmental chemical that has been linked to behavioral differences in children and shown to impact critical neurodevelopmental processes in animal models. Though data is emerging, we still have an incomplete picture of how BPA disrupts neurodevelopment; in particular, how its impacts may vary across different genetic backgrounds. Given the genetic tractability of Drosophila melanogaster, they present a valuable model to address this question. Fruit flies are increasingly being used for assessment of neurotoxicants because of their relatively simple brain structure and variety of measurable behaviors. Here we investigated the neurodevelopmental impacts of BPA across two genetic strains of Drosophila-w(1118) (control) and the Fragile X Syndrome (FXS) model-by examining both behavioral and neuronal phenotypes. We show that BPA induces hyperactivity in larvae, increases repetitive grooming behavior in adults, reduces courtship behavior, impairs axon guidance in the mushroom body, and disrupts neural stem cell development in the w(1118) genetic strain. Remarkably, for every behavioral and neuronal phenotype examined, the impact of BPA in FXS flies was either insignificant or contrasted with the phenotypes observed in the w(1118) strain. This data indicates that the neurodevelopmental impacts of BPA can vary widely depending on genetic background and suggests BPA may elicit a gene-environment interaction with Drosophila fragile X mental retardation 1 (dFmr1)-the ortholog of human FMR1, which causes Fragile X Syndrome and is associated with autism spectrum disorder. CI - Copyright (c) 2020 The Authors. Published by Elsevier B.V. All rights reserved. FAU - Nguyen, U AU - Nguyen U AD - Department of Biological Sciences, California State University, Sacramento, 6000 J Street, Sacramento, CA, 95819, United States. FAU - Tinsley, B AU - Tinsley B AD - Department of Biological Sciences, California State University, Sacramento, 6000 J Street, Sacramento, CA, 95819, United States. FAU - Sen, Y AU - Sen Y AD - Department of Biological Sciences, California State University, Sacramento, 6000 J Street, Sacramento, CA, 95819, United States. FAU - Stein, J AU - Stein J AD - Department of Biological Sciences, California State University, Sacramento, 6000 J Street, Sacramento, CA, 95819, United States. FAU - Palacios, Y AU - Palacios Y AD - Department of Biological Sciences, California State University, Sacramento, 6000 J Street, Sacramento, CA, 95819, United States. FAU - Ceballos, A AU - Ceballos A AD - Department of Biological Sciences, California State University, Sacramento, 6000 J Street, Sacramento, CA, 95819, United States. FAU - Welch, C AU - Welch C AD - Department of Biological Sciences, California State University, Sacramento, 6000 J Street, Sacramento, CA, 95819, United States. FAU - Nzenkue, K AU - Nzenkue K AD - Department of Biological Sciences, California State University, Sacramento, 6000 J Street, Sacramento, CA, 95819, United States. FAU - Penn, A AU - Penn A AD - Department of Biological Sciences, California State University, Sacramento, 6000 J Street, Sacramento, CA, 95819, United States. FAU - Murphy, L AU - Murphy L AD - Department of Biological Sciences, California State University, Sacramento, 6000 J Street, Sacramento, CA, 95819, United States. FAU - Leodones, K AU - Leodones K AD - Department of Biological Sciences, California State University, Sacramento, 6000 J Street, Sacramento, CA, 95819, United States. FAU - Casiquin, J AU - Casiquin J AD - Department of Biological Sciences, California State University, Sacramento, 6000 J Street, Sacramento, CA, 95819, United States. FAU - Ivory, I AU - Ivory I AD - Department of Biological Sciences, California State University, Sacramento, 6000 J Street, Sacramento, CA, 95819, United States. FAU - Ghenta, K AU - Ghenta K AD - Department of Biological Sciences, California State University, Sacramento, 6000 J Street, Sacramento, CA, 95819, United States. FAU - Danziger, K AU - Danziger K AD - Department of Biological Sciences, California State University, Sacramento, 6000 J Street, Sacramento, CA, 95819, United States. FAU - Widman, E AU - Widman E AD - Department of Biological Sciences, California State University, Sacramento, 6000 J Street, Sacramento, CA, 95819, United States. FAU - Newman, J AU - Newman J AD - Department of Biological Sciences, California State University, Sacramento, 6000 J Street, Sacramento, CA, 95819, United States. FAU - Triplehorn, M AU - Triplehorn M AD - Department of Biological Sciences, California State University, Sacramento, 6000 J Street, Sacramento, CA, 95819, United States. FAU - Hindi, Z AU - Hindi Z AD - Department of Biological Sciences, California State University, Sacramento, 6000 J Street, Sacramento, CA, 95819, United States. FAU - Mulligan, K AU - Mulligan K AD - Department of Biological Sciences, California State University, Sacramento, 6000 J Street, Sacramento, CA, 95819, United States. Electronic address: kimberly.mulligan@csus.edu. LA - eng GR - SC2 GM132005/GM/NIGMS NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural DEP - 20201210 PL - Netherlands TA - Neurotoxicology JT - Neurotoxicology JID - 7905589 RN - 0 (Benzhydryl Compounds) RN - 0 (Phenols) RN - MLT3645I99 (bisphenol A) SB - IM MH - Animals MH - Benzhydryl Compounds/*toxicity MH - Courtship MH - Disease Models, Animal MH - Drosophila melanogaster/*drug effects/genetics/growth & development MH - Female MH - Fragile X Syndrome/genetics/veterinary MH - Grooming/drug effects MH - Larva/drug effects/physiology MH - Locomotion/drug effects MH - Male MH - Nervous System/*drug effects/growth & development MH - Phenols/*toxicity PMC - PMC8075300 MID - NIHMS1688971 OTO - NOTNLM OT - Axon guidance OT - Behavior OT - Bisphenol A OT - Drosophila melanogaster OT - Fragile X syndrome OT - Neuroblasts EDAT- 2020/12/15 06:00 MHDA- 2021/10/30 06:00 PMCR- 2021/04/26 CRDT- 2020/12/14 10:17 PHST- 2020/07/27 00:00 [received] PHST- 2020/11/30 00:00 [revised] PHST- 2020/12/06 00:00 [accepted] PHST- 2020/12/15 06:00 [pubmed] PHST- 2021/10/30 06:00 [medline] PHST- 2020/12/14 10:17 [entrez] PHST- 2021/04/26 00:00 [pmc-release] AID - S0161-813X(20)30214-X [pii] AID - 10.1016/j.neuro.2020.12.007 [doi] PST - ppublish SO - Neurotoxicology. 2021 Jan;82:146-157. doi: 10.1016/j.neuro.2020.12.007. Epub 2020 Dec 10.