PMID- 33314673
OWN - NLM
STAT- MEDLINE
DCOM- 20210712
LR  - 20231107
IS  - 1864-0648 (Electronic)
IS  - 1864-063X (Print)
IS  - 1864-063X (Linking)
VI  - 14
IP  - 4
DP  - 2021 Apr
TI  - Exploring the utility of Deep Red Anthraquinone 5 for digital staining of ex vivo 
      confocal micrographs of optically sectioned skin.
PG  - e202000207
LID - 10.1002/jbio.202000207 [doi]
AB  - We investigated the utility of the fluorescent dye Deep Red Anthraquinone 5 
      (DRAQ5) for digital staining of optically sectioned skin in comparison to 
      acridine orange (AO). Eight fresh-frozen thawed Mohs discard tissue specimens 
      were stained with AO and DRAQ5, and imaged using an ex vivo confocal microscope 
      at three wavelengths (488 nm and 638 nm for fluorescence, 785 nm for 
      reflectance). Images were overlaid (AO + Reflectance, DRAQ5 + Reflectance), 
      digitally stained, and evaluated by three investigators for perceived image 
      quality (PIQ) and histopathological feature identification. In addition to 
      nuclear staining, AO seemed to stain dermal fibers in a subset of cases in 
      digitally stained images, while DRAQ5 staining was more specific to nuclei. 
      Blinded evaluation showed substantial agreement, favoring DRAQ5 for PIQ (82%, Cl 
      75%-90%, Gwet's AC 0.74) and for visualization of histopathological features in 
      (81%, Cl 73%-89%, Gwet's AC 0.67), supporting its use in digital staining of 
      multimodal confocal micrographs of skin.
CI  - (c) 2020 Wiley-VCH GmbH.
FAU - Ortner, Vinzent Kevin
AU  - Ortner VK
AUID- ORCID: 0000-0001-9708-6717
AD  - Department of Dermatology, Copenhagen University Hospital, Frederiskberg, 
      Denmark.
FAU - Sahu, Aditi
AU  - Sahu A
AUID- ORCID: 0000-0003-0004-3839
AD  - Dermatology Service, Memorial Sloan Kettering Cancer Center, New York, New York, 
      USA.
FAU - Cordova, Miguel
AU  - Cordova M
AUID- ORCID: 0000-0002-2962-0671
AD  - Dermatology Service, Memorial Sloan Kettering Cancer Center, New York, New York, 
      USA.
FAU - Kose, Kivanc
AU  - Kose K
AUID- ORCID: 0000-0003-3185-2639
AD  - Dermatology Service, Memorial Sloan Kettering Cancer Center, New York, New York, 
      USA.
FAU - Aleissa, Saud
AU  - Aleissa S
AUID- ORCID: 0000-0003-4756-5888
AD  - Dermatology Service, Memorial Sloan Kettering Cancer Center, New York, New York, 
      USA.
FAU - Alessi-Fox, Christi
AU  - Alessi-Fox C
AUID- ORCID: 0000-0001-6064-6311
AD  - Caliber Imaging and Diagnostics Inc., Rochester, New York, USA.
FAU - Haedersdal, Merete
AU  - Haedersdal M
AUID- ORCID: 0000-0003-1250-2035
AD  - Department of Dermatology, Copenhagen University Hospital, Frederiskberg, 
      Denmark.
FAU - Rajadhyaksha, Milind
AU  - Rajadhyaksha M
AUID- ORCID: 0000-0002-6323-4547
AD  - Dermatology Service, Memorial Sloan Kettering Cancer Center, New York, New York, 
      USA.
FAU - Rossi, Anthony Mario
AU  - Rossi AM
AUID- ORCID: 0000-0003-2295-1934
AD  - Dermatology Service, Memorial Sloan Kettering Cancer Center, New York, New York, 
      USA.
LA  - eng
GR  - P30 CA008748/CA/NCI NIH HHS/United States
GR  - R01 EB020029/EB/NIBIB NIH HHS/United States
GR  - R01EB020029/EB/NIBIB NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20201228
PL  - Germany
TA  - J Biophotonics
JT  - Journal of biophotonics
JID - 101318567
RN  - 0 (Anthraquinones)
SB  - IM
MH  - Anthraquinones
MH  - Humans
MH  - Microscopy, Confocal
MH  - *Skin
MH  - *Skin Neoplasms
MH  - Staining and Labeling
PMC - PMC8274380
MID - NIHMS1717419
OTO - NOTNLM
OT  - DRAQ5
OT  - EVCM
OT  - Mohs
OT  - acridine orange
OT  - confocal microscopy
OT  - tissue fixture
COIS- Conflict of Interest Statement: Rossi AM: travel by Mavig Inc., distributor of 
      VivaScope System manufactured by Caliber ID Fox CA: employee and equity holder of 
      Caliber ID Rajadhyaksha M: holds equity in Caliber ID
EDAT- 2020/12/15 06:00
MHDA- 2021/07/13 06:00
PMCR- 2021/07/12
CRDT- 2020/12/14 11:16
PHST- 2020/12/10 00:00 [revised]
PHST- 2020/05/29 00:00 [received]
PHST- 2020/12/11 00:00 [accepted]
PHST- 2020/12/15 06:00 [pubmed]
PHST- 2021/07/13 06:00 [medline]
PHST- 2020/12/14 11:16 [entrez]
PHST- 2021/07/12 00:00 [pmc-release]
AID - 10.1002/jbio.202000207 [doi]
PST - ppublish
SO  - J Biophotonics. 2021 Apr;14(4):e202000207. doi: 10.1002/jbio.202000207. Epub 2020 
      Dec 28.