PMID- 33315153
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201217
IS  - 2191-0855 (Print)
IS  - 2191-0855 (Electronic)
IS  - 2191-0855 (Linking)
VI  - 10
IP  - 1
DP  - 2020 Dec 14
TI  - Oral administration of recombinant Bacillus subtilis spores expressing mutant 
      staphylococcal enterotoxin B provides potent protection against lethal 
      enterotoxin challenge.
PG  - 215
LID - 10.1186/s13568-020-01152-x [doi]
LID - 215
AB  - Pathogenicity of Staphylococcus aureus is induced by staphylococcal enterotoxin B 
      (SEB). A mutant form of SEB (mSEB) is immunogenic as well as less toxic. 
      Recombinant mSEB and SEB were expressed in pET28a prokaryotic plasmids. Tumor 
      necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) levels in mSEB-stimulated 
      macrophages were lower than those in SEB-stimulated macrophages (p < 0.001, 
      p < 0.01 respectively). Using CotC as a fusion protein, we constructed 
      recombinant Bacillus subtilis spores expressing mSEB on the spore surface and 
      evaluated their safety and protective efficacy via mouse models. Oral 
      administration of mSEB-expressing spores increased SEB-specific IgA in feces and 
      SEB-specific IgG1 and IgG2a in the sera, compared with mice in naive and CotC 
      spore-treated groups (p < 0.001, p < 0.01, p < 0.001 respectively). Six weeks 
      following oral dosing of recombinant spores, significant differences were not 
      found in the serum biochemical indices between the mSEB group and the naive and 
      CotC groups. Furthermore, oral administration of mSEB spores increased the 
      survival rate by 33.3% in mice intraperitoneally injected with 5 microg of wild-type 
      SEB plus 25 microg lipopolysaccharide (LPS). In summation, recombinant spores stably 
      expressing mSEB were developed, and oral administration of such recombinant 
      spores induced a humoral immune response and provided protection against SEB 
      challenge in mice.
FAU - Xiong, Zhile
AU  - Xiong Z
AD  - Clinical laboratory, Guangzhou Women and Children's Medical Center, Guangzhou 
      Medical University, Guangzhou, 510623, Guangdong, China.
FAU - Mai, Jialiang
AU  - Mai J
AD  - Clinical laboratory, Guangzhou Women and Children's Medical Center, Guangzhou 
      Medical University, Guangzhou, 510623, Guangdong, China.
FAU - Li, Fei
AU  - Li F
AD  - Clinical laboratory, Guangzhou Women and Children's Medical Center, Guangzhou 
      Medical University, Guangzhou, 510623, Guangdong, China.
FAU - Liang, Bingshao
AU  - Liang B
AD  - Clinical laboratory, Guangzhou Women and Children's Medical Center, Guangzhou 
      Medical University, Guangzhou, 510623, Guangdong, China.
FAU - Yao, Shuwen
AU  - Yao S
AD  - Clinical laboratory, Guangzhou Women and Children's Medical Center, Guangzhou 
      Medical University, Guangzhou, 510623, Guangdong, China.
FAU - Liang, Zhuwei
AU  - Liang Z
AD  - Clinical laboratory, Guangzhou Women and Children's Medical Center, Guangzhou 
      Medical University, Guangzhou, 510623, Guangdong, China.
FAU - Zhang, Chao
AU  - Zhang C
AD  - Clinical laboratory, Guangzhou Women and Children's Medical Center, Guangzhou 
      Medical University, Guangzhou, 510623, Guangdong, China.
FAU - Gao, Fei
AU  - Gao F
AD  - Clinical laboratory, Guangzhou Women and Children's Medical Center, Guangzhou 
      Medical University, Guangzhou, 510623, Guangdong, China.
FAU - Ai, Xiaolan
AU  - Ai X
AD  - Clinical laboratory, Guangzhou Women and Children's Medical Center, Guangzhou 
      Medical University, Guangzhou, 510623, Guangdong, China.
FAU - Wang, Jielin
AU  - Wang J
AD  - Clinical laboratory, Guangzhou Women and Children's Medical Center, Guangzhou 
      Medical University, Guangzhou, 510623, Guangdong, China.
FAU - Long, Yan
AU  - Long Y
AD  - Clinical laboratory, Guangzhou Women and Children's Medical Center, Guangzhou 
      Medical University, Guangzhou, 510623, Guangdong, China.
FAU - Yang, Min
AU  - Yang M
AD  - Department of Gastroenterology, Guangzhou Women and Children's Medical Center, 
      Guangzhou Medical University, Guangzhou, 510623, Guangdong, China.
FAU - Gong, Sitang
AU  - Gong S
AD  - Department of Gastroenterology, Guangzhou Women and Children's Medical Center, 
      Guangzhou Medical University, Guangzhou, 510623, Guangdong, China.
FAU - Zhou, Zhenwen
AU  - Zhou Z
AD  - Clinical laboratory, Guangzhou Women and Children's Medical Center, Guangzhou 
      Medical University, Guangzhou, 510623, Guangdong, China. zzw6248@126.com.
LA  - eng
GR  - 30801054/National Natural Science Foundation of China/
GR  - no.2016A020215013 and no. 2014A020212013/the Guangdong Science and Technology 
      Foundation/
GR  - No. 201102A212013, No. 20131A011053, and No. 20171A010267/the Medical Health 
      Science and Technology Foundation of Guangzhou/
GR  - No. 201707010010/Science and Technology Foundation of Guangzhou/
GR  - No. 20181A011039/Guangzhou Health and Family Planning Science and Technology 
      Project/
GR  - No. 8451012001001570 and No. 915101200F009/the Natural Science Foundation of 
      Guangdong/
GR  - Nos. Pre-NSFC-2019-014 and IP-2019-022/Paediatric Institute Foundation of 
      Guangzhou Women and Children's Medical Center/
PT  - Journal Article
DEP - 20201214
PL  - Germany
TA  - AMB Express
JT  - AMB Express
JID - 101561785
PMC - PMC7734462
OTO - NOTNLM
OT  - Bacillus subtilis
OT  - Staphylococcal enterotoxin B
OT  - Staphylococcus aureus
OT  - Vaccination
COIS- The authors all declare that they have no competing interests.
EDAT- 2020/12/15 06:00
MHDA- 2020/12/15 06:01
PMCR- 2020/12/14
CRDT- 2020/12/14 12:09
PHST- 2020/09/20 00:00 [received]
PHST- 2020/11/25 00:00 [accepted]
PHST- 2020/12/14 12:09 [entrez]
PHST- 2020/12/15 06:00 [pubmed]
PHST- 2020/12/15 06:01 [medline]
PHST- 2020/12/14 00:00 [pmc-release]
AID - 10.1186/s13568-020-01152-x [pii]
AID - 1152 [pii]
AID - 10.1186/s13568-020-01152-x [doi]
PST - epublish
SO  - AMB Express. 2020 Dec 14;10(1):215. doi: 10.1186/s13568-020-01152-x.