PMID- 33315353
OWN - NLM
STAT- MEDLINE
DCOM- 20211223
LR  - 20211223
IS  - 1303-6165 (Electronic)
IS  - 1300-0144 (Print)
IS  - 1300-0144 (Linking)
VI  - 51
IP  - 3
DP  - 2021 Jun 28
TI  - Is cervical swab an efficient method for developing a new noninvasive prenatal 
      diagnostic test for numerical and structural chromosome anomalies?
PG  - 1043-1048
LID - 10.3906/sag-2009-347 [doi]
AB  - BACKGROUND/AIM: Prenatal diagnosis is vital to obtain healthy generation for 
      risky pregnancies. There have been several approaches, some of which are 
      routinely applied in clinics to evaluate the possible prenatal deficiencies 
      and/or diseases. In the present study, we aimed to isolate the fetal cells from 
      endocervical samples and try to identify possible anomalies which were proved by 
      Amniocentesis (AS) and chorionic villus sampling (CVS) methods. MATERIALS AND 
      METHODS: Endoservical specimens were collected from 100 pregnant women. Cells 
      were separated in parallel by fluorescence-activated cell sorting (FACS) and 
      magnetic-activated cell sorting (MACS) using human leukocyte antigen (HLA) G233 
      and placental alkaline phosphatase (PLAP) antibodies. CMA (comprehensive 
      meta-analysis) were carried out and male fetuses were confirmed with Sex 
      determining region Y (SRY) amplification. RESULTS: The percent of HLA G233 and 
      placental and placental alkaline phosphatase (PLAP) positive cells were 4.55% and 
      84.59%, respectively. The percent of cells positive for both markers was 14.75%. 
      CMA analyses were not informative. (SRY) was amplified in 67% of the samples. 
      CONCLUSION: However, the success rate of the both cell sorting and scanning of 
      DNA anomalies by aCGH and/or RT-PCR was limited, preventing the applicability of 
      this proposal in the clinics. Still, the success of the proposed method depends 
      on the development of the novel fetal cell-specific antibodies and the 
      improvements in the sorting systems.
CI  - This work is licensed under a Creative Commons Attribution 4.0 International 
      License.
FAU - Yurtcu, Erkan
AU  - Yurtcu E
AUID- ORCID: 0000-0003-4930-8164
AD  - Department of Medical Biology, Faculty of Medicine, Baskent University,Ankara, 
      Turkey
FAU - Karcaaltincaba, Deniz
AU  - Karcaaltincaba D
AUID- ORCID: 0000-0001-5276-9303
AD  - Department of Obstetrics and Gynecology, Faculty of Medicine, Gazi University, 
      Ankara, Turkey
FAU - Kazan, Hasan Huseyin
AU  - Kazan HH
AUID- ORCID: 0000-0001-7936-8606
AD  - Department of Medical Genetics, Faculty of Medicine, Gazi University, Ankara, 
      Turkey
FAU - Ozdemir, Halis
AU  - Ozdemir H
AUID- ORCID: 0000-0002-9194-8504
AD  - Department of Obstetrics and Gynecology, Faculty of Medicine, Gazi University, 
      Ankara, Turkey
FAU - Yirmibes Karaoguz, Meral
AU  - Yirmibes Karaoguz M
AUID- ORCID: 0000-0002-0178-8018
AD  - Department of Medical Genetics, Faculty of Medicine, Gazi University, Ankara, 
      Turkey
FAU - Calis, Pinar
AU  - Calis P
AUID- ORCID: 0000-0001-9334-1987
AD  - Department of Obstetrics and Gynaecology, Sami Ulus Women's and Children's Health 
      Training and Research Hospital, Ankara, Turkey
FAU - Kayhan, Gulsum
AU  - Kayhan G
AUID- ORCID: 0000-0002-4286-243X
AD  - Department of Medical Genetics, Faculty of Medicine, Gazi University, Ankara, 
      Turkey
FAU - Guntekin Ergun, Sezen
AU  - Guntekin Ergun S
AUID- ORCID: 0000-0002-7133-5049
AD  - Department of Medical Biology, Faculty of Medicine, Hacettepe University, Ankara, 
      Turkey
FAU - Percin, Ferda
AU  - Percin F
AUID- ORCID: 0000-0001-9317-8155
AD  - Department of Medical Genetics, Faculty of Medicine, Gazi University, Ankara, 
      Turkey
FAU - Bayram, Merih
AU  - Bayram M
AUID- ORCID: 0000-0003-1299-2433
AD  - Department of Obstetrics and Gynecology, Faculty of Medicine, Gazi University, 
      Ankara, Turkey
FAU - Ilhan, Mustafa Necmi
AU  - Ilhan MN
AUID- ORCID: 0000-0003-1367-6328
AD  - Department of Public Health, Faculty of Medicine, Gazi University, Ankara, Turkey
FAU - Bilgili, Gamze
AU  - Bilgili G
AUID- ORCID: 0000-0002-4233-2738
AD  - Department of Medical Biology, Faculty of Medicine, Baskent University,Ankara, 
      Turkey
FAU - Kaymak, Tugrul
AU  - Kaymak T
AUID- ORCID: 0000-0001-5880-8211
AD  - Mycotoxin Analysis Laboratory, Ministry of Agriculture and Forestry, Ankara, 
      Turkey
FAU - Ergun, Mehmet Ali
AU  - Ergun MA
AUID- ORCID: 0000-0001-9696-0433
AD  - Department of Medical Genetics, Faculty of Medicine, Gazi University, Ankara, 
      Turkey
LA  - eng
PT  - Journal Article
PT  - Meta-Analysis
DEP - 20210628
PL  - Turkey
TA  - Turk J Med Sci
JT  - Turkish journal of medical sciences
JID - 9441758
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - *Alkaline Phosphatase
MH  - Chromosome Aberrations
MH  - Chromosomes
MH  - *Diagnostic Tests, Routine
MH  - Female
MH  - Humans
MH  - Male
MH  - Placenta
MH  - Pregnancy
MH  - Prenatal Diagnosis
PMC - PMC8283453
OTO - NOTNLM
OT  - Cervical swab
OT  - CMA (comprehensive meta-analysis)
OT  - fluorescence-activated cell sorting (FACS)
OT  - magnetic-activated cell sorting (MACS)
COIS- none declared
EDAT- 2020/12/15 06:00
MHDA- 2021/12/24 06:00
PMCR- 2021/06/28
CRDT- 2020/12/14 13:35
PHST- 2020/09/30 00:00 [received]
PHST- 2020/12/12 00:00 [accepted]
PHST- 2020/12/14 13:35 [entrez]
PHST- 2020/12/15 06:00 [pubmed]
PHST- 2021/12/24 06:00 [medline]
PHST- 2021/06/28 00:00 [pmc-release]
AID - 10.3906/sag-2009-347 [doi]
PST - epublish
SO  - Turk J Med Sci. 2021 Jun 28;51(3):1043-1048. doi: 10.3906/sag-2009-347.