PMID- 33316768
OWN - NLM
STAT- MEDLINE
DCOM- 20210824
LR  - 20210824
IS  - 1899-1505 (Electronic)
IS  - 0867-5910 (Linking)
VI  - 71
IP  - 4
DP  - 2020 Aug
TI  - The impact of 3,4-methylendioxymetamphetamine (MDMA) abstinence on seeking 
      behavior and the expression of the D(2)-like and mGlu(5) receptors in the rat 
      brain using saturation binding analyses.
LID - 10.26402/jpp.2020.4.09 [doi]
AB  - The abundance of research indicates that enriched environment acts as a 
      beneficial factor reducing the risks of relapse in substance use disorder. There 
      is also strong evidence showing the engagement of brain dopaminergic and 
      glutamatergic signaling through the dopamine D(2)-like and metabotropic glutamate 
      type 5 (mGlu(5)) receptors, respectively, that has a direct impact on drug reward 
      and drug abstinence. The present study involved 3,4-methylendioxymethamphetamine 
      (MDMA) self-administration with the yoked-triad procedure in rats kept under 
      different housing conditions during abstinence - enriched environment (EE) or 
      isolation cage (IC) conditions - aimed at evaluating changes in brain receptors 
      affecting drug-seeking behavior as well as density and affinity of the D(2)-like 
      and mGlu(5) receptors in several regions of the animal brain. Our results show 
      that exposure to EE conditions strongly reduced active lever presses during 
      cue-induced drug-seeking. At the neurochemical level, we demonstrated marked 
      decreases of D(2)-like receptor affinity in the dorsal striatum in rats 
      previously self-administering MDMA under EE and increases in density under IC 
      conditions. Moreover, we found the increases in the density and decreases in the 
      affinity of the D(2)-like receptor in the prefrontal cortex and nucleus accumbens 
      provoked by IC conditions. The mGlu(5) receptor density decreased only in the 
      prefrontal cortex after IC and EE abstinence. Moreover, our study has revealed a 
      clear decrease in mGlu(5) receptor density in the nucleus accumbens in the group 
      actively administering MDMA only under EE conditions. This study demonstrates 
      that housing conditions have impact on drug-seeking behavior in rats during 
      abstinence from MDMA self-administration. The observed changes in the dopamine 
      D(2)-like and mGlu(5) receptor B(max) and/or K(d) values were brain-region 
      specific and related to either pharmacological and/or motivational features of 
      MDMA.
FAU - Frankowska, M
AU  - Frankowska M
AD  - Maj Institute of Pharmacology, Polish Academy of Sciences, Department of Drug 
      Addiction Pharmacology, Cracow, Poland. frankow@if-pan.krakow.pl.
FAU - Miszkiel, J
AU  - Miszkiel J
AD  - Maj Institute of Pharmacology, Polish Academy of Sciences, Department of Drug 
      Addiction Pharmacology, Cracow, Poland.
FAU - Pomierny-Chamiolo, L
AU  - Pomierny-Chamiolo L
AD  - Department of Toxicology, Faculty of Pharmacy, Jagiellonian University Collegium 
      Medicum, Cracow, Poland.
FAU - Pomierny, B
AU  - Pomierny B
AD  - Department of Toxicology, Faculty of Pharmacy, Jagiellonian University Collegium 
      Medicum, Cracow, Poland.
FAU - Celeste Borelli, A
AU  - Celeste Borelli A
AD  - Department of Medical Sciences, University of Ferrara, Ferrara, Italy.
FAU - Suder, A
AU  - Suder A
AD  - Maj Institute of Pharmacology, Polish Academy of Sciences, Department of Drug 
      Addiction Pharmacology, Cracow, Poland.
FAU - Filip, M
AU  - Filip M
AD  - Maj Institute of Pharmacology, Polish Academy of Sciences, Department of Drug 
      Addiction Pharmacology, Cracow, Poland.
LA  - eng
PT  - Journal Article
DEP - 20201212
PL  - Poland
TA  - J Physiol Pharmacol
JT  - Journal of physiology and pharmacology : an official journal of the Polish 
      Physiological Society
JID - 9114501
RN  - 0 (Central Nervous System Stimulants)
RN  - 0 (Grm5 protein, rat)
RN  - 0 (Receptor, Metabotropic Glutamate 5)
RN  - 0 (Receptors, Dopamine D2)
RN  - 0 (dopamine D2L receptor)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
SB  - IM
MH  - Amphetamine-Related Disorders/*metabolism/physiopathology/psychology
MH  - Animals
MH  - Behavior, Animal/*drug effects
MH  - Brain/*drug effects/metabolism/physiopathology
MH  - Central Nervous System Stimulants/*pharmacology
MH  - Cues
MH  - Drug-Seeking Behavior/*drug effects
MH  - Housing, Animal
MH  - Male
MH  - N-Methyl-3,4-methylenedioxyamphetamine/*pharmacology
MH  - Rats, Wistar
MH  - Receptor, Metabotropic Glutamate 5/*metabolism
MH  - Receptors, Dopamine D2/*metabolism
MH  - Social Isolation
EDAT- 2020/12/15 06:00
MHDA- 2021/08/25 06:00
CRDT- 2020/12/14 20:15
PHST- 2020/08/06 00:00 [received]
PHST- 2020/08/30 00:00 [accepted]
PHST- 2020/12/14 20:15 [entrez]
PHST- 2020/12/15 06:00 [pubmed]
PHST- 2021/08/25 06:00 [medline]
AID - 10.26402/jpp.2020.4.09 [doi]
PST - ppublish
SO  - J Physiol Pharmacol. 2020 Aug;71(4). doi: 10.26402/jpp.2020.4.09. Epub 2020 Dec 
      12.