PMID- 33318310
OWN - NLM
STAT- MEDLINE
DCOM- 20210510
LR  - 20210510
IS  - 1945-4589 (Electronic)
IS  - 1945-4589 (Linking)
VI  - 13
IP  - 2
DP  - 2020 Dec 9
TI  - Matrix stiffness regulates myocardial differentiation of human umbilical cord 
      mesenchymal stem cells.
PG  - 2231-2250
LID - 10.18632/aging.202244 [doi]
AB  - Myocardial infarction is a cardiovascular disease with high mortality. Human 
      umbilical cord mesenchymal stem cells (hUC-MSCs) with strong self-renewal 
      capacity and multipotency, provide the possibility of replacing injured 
      cardiomyocytes. hUC-MSCs were cultured on polyacrylamide hydrogels with 
      stiffnesses corresponding to Young's modulus of 13-16kPa and 62-68kPa which mimic 
      the stiffnesses of healthy heart tissue and fibrotic myocardium. The expression 
      of early myocardial markers Nkx2.5, GATA4, Mesp1 and the mature myocardial 
      markers cTnT, cTnI, alpha-actin were detected by RT-PCR and Western Blot, which 
      showed that soft matrix (13-16 kPa) tended to induce the differentiation of 
      hUC-MSCs into myocardium, compared with stiff matrix (62-68 kPa). Piezos are 
      mechanically sensitive non-selective cation channels. The expression of Piezo1 
      increased with the stiffness gradient of 1-10kPa, 13-16kPa, 35-38kPa and 62-68kPa 
      on the 1(st) day, but Piezo2 expression was irregular. The expression of integrin 
      beta1 and calcium ions were also higher on stiff substrate than on soft substrate. 
      hUC-MSCs tend to differentiate into myocardium on the matrix stiffness of 13-16 
      kPa. The relationship among matrix stiffness, Piezo1 and myocardial 
      differentiation needs further validation.
FAU - Sun, Yingying
AU  - Sun Y
AD  - Department of Stomatology, The First Hospital of Jilin University, Jilin 
      University, Changchun, China.
AD  - The Key Laboratory of Pathobiology, Ministry of Education, College of Basic 
      Medical Sciences, Jilin University, Changchun, China.
FAU - Liu, Jingwei
AU  - Liu J
AD  - College of Clinical Medicine, Jilin University, Changchun, China.
FAU - Xu, Ziran
AU  - Xu Z
AD  - The Key Laboratory of Pathobiology, Ministry of Education, College of Basic 
      Medical Sciences, Jilin University, Changchun, China.
FAU - Lin, Xiaoxuan
AU  - Lin X
AD  - The Key Laboratory of Pathobiology, Ministry of Education, College of Basic 
      Medical Sciences, Jilin University, Changchun, China.
FAU - Zhang, Xiaoling
AU  - Zhang X
AD  - Key Laboratory of Organ Regeneration and Transplantation of Ministry of 
      Education, The First Hospital, Jilin University, Changchun, China.
AD  - National-Local Joint Engineering Laboratory of Animal Models for Human Diseases, 
      Changchun, China.
FAU - Li, Lisha
AU  - Li L
AD  - The Key Laboratory of Pathobiology, Ministry of Education, College of Basic 
      Medical Sciences, Jilin University, Changchun, China.
FAU - Li, Yulin
AU  - Li Y
AD  - The Key Laboratory of Pathobiology, Ministry of Education, College of Basic 
      Medical Sciences, Jilin University, Changchun, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201209
PL  - United States
TA  - Aging (Albany NY)
JT  - Aging
JID - 101508617
RN  - 0 (Hydrogels)
SB  - IM
MH  - Cell Differentiation/*physiology
MH  - Cells, Cultured
MH  - Elastic Modulus
MH  - Extracellular Matrix/*metabolism
MH  - Female
MH  - Humans
MH  - Hydrogels
MH  - Mesenchymal Stem Cells/*cytology/metabolism
MH  - Myocardial Infarction/metabolism
MH  - Myocardium/*cytology/metabolism
MH  - Myocytes, Cardiac/*metabolism
MH  - Umbilical Cord/*cytology/metabolism
PMC - PMC7880396
OTO - NOTNLM
OT  - Piezo1
OT  - human umbilical cord mesenchymal stem cells
OT  - integrin beta1
OT  - matrix stiffness
OT  - myocardial differentiation
COIS- CONFLICTS OF INTEREST: The authors declare that they have no conflicts of 
      interest.
EDAT- 2020/12/16 06:00
MHDA- 2021/05/11 06:00
PMCR- 2021/01/31
CRDT- 2020/12/15 05:55
PHST- 2020/04/30 00:00 [received]
PHST- 2020/10/20 00:00 [accepted]
PHST- 2020/12/16 06:00 [pubmed]
PHST- 2021/05/11 06:00 [medline]
PHST- 2020/12/15 05:55 [entrez]
PHST- 2021/01/31 00:00 [pmc-release]
AID - 202244 [pii]
AID - 10.18632/aging.202244 [doi]
PST - ppublish
SO  - Aging (Albany NY). 2020 Dec 9;13(2):2231-2250. doi: 10.18632/aging.202244. Epub 
      2020 Dec 9.