PMID- 33319055
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220419
IS  - 2397-9070 (Electronic)
IS  - 2397-9070 (Linking)
VI  - 4
IP  - 6
DP  - 2020 Dec
TI  - Effect of cigarette smoke extract on the intestinal microenvironment of 
      ulcerative colitis tissue.
PG  - 1191-1198
LID - 10.1002/jgh3.12422 [doi]
AB  - BACKGROUND AND AIM: Ulcerative colitis (UC) is an autoimmune disease 
      characterized by inflammation in the gastrointestinal tract. The severity of UC 
      is higher in nonsmokers than smokers; however, the biological mechanisms 
      controlling this effect remain unknown. The aim of this study was to examine the 
      effect of cigarette smoke extract (CSE) on inflamed and noninflamed colonic 
      tissue from UC patients and to determine if inflammatory mediators, transcription 
      factors, and T cell phenotypes are altered by CSE. METHODS: Blood and colonic 
      biopsies were obtained from UC patients undergoing endoscopy. Biopsies were 
      cultured in the presence or absence of CSE. Multiplex enzyme-linked immunosorbent 
      assay (ELISA) measured secreted levels of inflammatory mediators. Nuclear factor 
      kappa-light-chain-enhancer of activated B cells (NF-kappaB) and Hypoxia-inducible 
      factor 1-alpha (HIF-1alpha) expression were measured by DNA-binding ELISA. T cell 
      phenotypes were assessed by flow cytometry in matched blood and biopsies. 
      RESULTS: Secreted levels of interleukin 2 (IL-2), interleukin 6 (IL-6), tumor 
      necrosis factor - alpha (TNF-alpha), chemokine (C-C motif) ligand 2 (CCL2), and 
      interleukin 10 (IL-10) were significantly (all P < 0.05) decreased following 
      treatment with CSE. This effect was specific to inflamed tissue and was not 
      observed in noninflamed tissue. CSE did not alter the expression of NF-kappaB or 
      HIF-1alpha. Assessment of T cell phenotypes in blood and tissue revealed that there 
      were significantly more activated and exhausted T cells in the colonic tissue 
      compared to matched blood. These profiles were not altered following CSE 
      treatment. CONCLUSION: These data suggest that observed effects of CSE in 
      reducing inflammatory mediators ex vivo are specific to inflamed colonic tissue 
      but are not due to the activation of NF-kappaB or HIF-1alpha and are not caused by 
      alterations in subpopulations of T cells in these UC tissues.
CI  - (c) 2020 The Authors. JGH Open: An open access journal of gastroenterology and 
      hepatology published by Journal of Gastroenterology and Hepatology Foundation and 
      John Wiley & Sons Australia, Ltd.
FAU - Cannon, Aoife
AU  - Cannon A
AUID- ORCID: 0000-0002-7739-5528
AD  - Department of Surgery Trinity Translational Medicine Institute, Trinity College 
      Dublin Dublin Ireland.
FAU - Clarke, Niamh
AU  - Clarke N
AD  - Department of Surgery Trinity Translational Medicine Institute, Trinity College 
      Dublin Dublin Ireland.
FAU - MacCarthy, Finbar
AU  - MacCarthy F
AD  - Department of Clinical Medicine Trinity Translational Medicine Institute, St. 
      James's Hospital Dublin Ireland.
AD  - Department of Gastroenterology SACC Directorate, St. James's Hospital Dublin 
      Ireland.
FAU - Dunne, Cara
AU  - Dunne C
AD  - Department of Clinical Medicine Trinity Translational Medicine Institute, St. 
      James's Hospital Dublin Ireland.
AD  - Department of Gastroenterology SACC Directorate, St. James's Hospital Dublin 
      Ireland.
FAU - Kevans, David
AU  - Kevans D
AD  - Department of Clinical Medicine Trinity Translational Medicine Institute, St. 
      James's Hospital Dublin Ireland.
AD  - Department of Gastroenterology SACC Directorate, St. James's Hospital Dublin 
      Ireland.
FAU - Mahmud, Nasir
AU  - Mahmud N
AD  - Department of Clinical Medicine Trinity Translational Medicine Institute, St. 
      James's Hospital Dublin Ireland.
AD  - Department of Gastroenterology SACC Directorate, St. James's Hospital Dublin 
      Ireland.
FAU - Lysaght, Joanne
AU  - Lysaght J
AD  - Department of Surgery Trinity Translational Medicine Institute, Trinity College 
      Dublin Dublin Ireland.
FAU - O'Sullivan, Jacintha
AU  - O'Sullivan J
AD  - Department of Surgery Trinity Translational Medicine Institute, Trinity College 
      Dublin Dublin Ireland.
LA  - eng
PT  - Journal Article
DEP - 20201021
PL  - Australia
TA  - JGH Open
JT  - JGH open : an open access journal of gastroenterology and hepatology
JID - 101730833
PMC - PMC7731828
OTO - NOTNLM
OT  - T lymphocytes
OT  - cigarette smoke extract
OT  - inflammatory bowel disease
OT  - inflammatory proteins
OT  - smoking
EDAT- 2020/12/16 06:00
MHDA- 2020/12/16 06:01
PMCR- 2020/10/21
CRDT- 2020/12/15 06:05
PHST- 2019/10/17 00:00 [received]
PHST- 2020/03/21 00:00 [revised]
PHST- 2020/09/20 00:00 [accepted]
PHST- 2020/12/15 06:05 [entrez]
PHST- 2020/12/16 06:00 [pubmed]
PHST- 2020/12/16 06:01 [medline]
PHST- 2020/10/21 00:00 [pmc-release]
AID - JGH312422 [pii]
AID - 10.1002/jgh3.12422 [doi]
PST - epublish
SO  - JGH Open. 2020 Oct 21;4(6):1191-1198. doi: 10.1002/jgh3.12422. eCollection 2020 
      Dec.