PMID- 33321671
OWN - NLM
STAT- MEDLINE
DCOM- 20210514
LR  - 20210514
IS  - 1873-0191 (Electronic)
IS  - 0928-4931 (Linking)
VI  - 119
DP  - 2021 Feb
TI  - Graphene oxide and electroactive reduced graphene oxide-based composite fibrous 
      scaffolds for engineering excitable nerve tissue.
PG  - 111632
LID - S0928-4931(20)33550-5 [pii]
LID - 10.1016/j.msec.2020.111632 [doi]
AB  - This study systematically investigates the role of graphene oxide (GO) and 
      reduced GO (rGO)/silk-based composite micro/nano-fibrous scaffolds in regulating 
      neuronal cell behavior in vitro, given the limited comparative studies on the 
      effects of graphene family materials on nerve regeneration. Fibrous scaffolds can 
      mimic the architecture of the native extracellular matrix and are potential 
      candidates for tissue engineering peripheral nerves. Silk/GO micro/nano-fibrous 
      scaffolds were electrospun with GO loadings 1 to 10 wt.%, and optionally 
      post-reduced in situ to explore a family of electrically conductive non-woven 
      silk/rGO scaffolds. Conductivities up to 4 x 10(-5) S cm(-1) were recorded in the 
      dry state, which increased up to 3 x 10(-4) S cm(-1) after hydration. Neuronoma 
      NG108-15 cells adhered and were viable on all substrates. Enhanced metabolic 
      activity and proliferation were observed on the GO-containing scaffolds, and 
      these cell responses were further promoted for electroactive silk/rGO. Neurite 
      extensions up to 100 mum were achieved by day 5, with maximum outgrowth up to 
      ~250 mum on some of the conductive substrates. These electroactive composite 
      fibrous scaffolds exhibit potential to enhance the neuronal cell response and 
      could be versatile supportive substrates for neural tissue engineering 
      applications.
CI  - Copyright (c) 2020. Published by Elsevier B.V.
FAU - Magaz, Adrian
AU  - Magaz A
AD  - Department of Materials and Henry Royce Institute, The University of Manchester, 
      Manchester M13 9PL, United Kingdom; Institute of Materials Research and 
      Engineering (IMRE), Agency for Science Technology and Research (A*STAR), 138634, 
      Singapore.
FAU - Li, Xu
AU  - Li X
AD  - Institute of Materials Research and Engineering (IMRE), Agency for Science 
      Technology and Research (A*STAR), 138634, Singapore; Department of Chemistry, 
      National University of Singapore, 117543 Singapore, Singapore. Electronic 
      address: x-li@imre.a-star.edu.sg.
FAU - Gough, Julie E
AU  - Gough JE
AD  - Department of Materials and Henry Royce Institute, The University of Manchester, 
      Manchester M13 9PL, United Kingdom.
FAU - Blaker, Jonny J
AU  - Blaker JJ
AD  - Department of Materials and Henry Royce Institute, The University of Manchester, 
      Manchester M13 9PL, United Kingdom; Department of Biomaterials, Institute of 
      Clinical Dentistry, University of Oslo, Oslo 0317, Norway. Electronic address: 
      jonny.blaker@manchester.ac.uk.
LA  - eng
PT  - Journal Article
DEP - 20201016
PL  - Netherlands
TA  - Mater Sci Eng C Mater Biol Appl
JT  - Materials science & engineering. C, Materials for biological applications
JID - 101484109
RN  - 0 (graphene oxide)
RN  - 7782-42-5 (Graphite)
RN  - 9007-76-5 (Fibroins)
SB  - IM
MH  - *Fibroins
MH  - *Graphite
MH  - *Nerve Tissue
MH  - Tissue Engineering
MH  - Tissue Scaffolds
OTO - NOTNLM
OT  - Electrospinning
OT  - Graphene oxide
OT  - Neuronal cells
OT  - Neuronal scaffold
OT  - Reduced graphene oxide
OT  - Silk fibroin
EDAT- 2020/12/17 06:00
MHDA- 2021/05/15 06:00
CRDT- 2020/12/16 01:01
PHST- 2020/07/16 00:00 [received]
PHST- 2020/10/02 00:00 [revised]
PHST- 2020/10/13 00:00 [accepted]
PHST- 2020/12/16 01:01 [entrez]
PHST- 2020/12/17 06:00 [pubmed]
PHST- 2021/05/15 06:00 [medline]
AID - S0928-4931(20)33550-5 [pii]
AID - 10.1016/j.msec.2020.111632 [doi]
PST - ppublish
SO  - Mater Sci Eng C Mater Biol Appl. 2021 Feb;119:111632. doi: 
      10.1016/j.msec.2020.111632. Epub 2020 Oct 16.