PMID- 33322080
OWN - NLM
STAT- MEDLINE
DCOM- 20210722
LR  - 20210722
IS  - 2073-4425 (Electronic)
IS  - 2073-4425 (Linking)
VI  - 11
IP  - 12
DP  - 2020 Dec 10
TI  - The Puzzling Fate of a Lupin Chromosome Revealed by Reciprocal Oligo-FISH and 
      BAC-FISH Mapping.
LID - 10.3390/genes11121489 [doi]
LID - 1489
AB  - Old World lupins constitute an interesting model for evolutionary research due to 
      diversity in genome size and chromosome number, indicating evolutionary genome 
      reorganization. It has been hypothesized that the polyploidization event which 
      occurred in the common ancestor of the Fabaceae family was followed by a 
      lineage-specific whole genome triplication (WGT) in the lupin clade, driving 
      chromosome rearrangements. In this study, chromosome-specific markers were used 
      as probes for heterologous fluorescence in situ hybridization (FISH) to identify 
      and characterize structural chromosome changes among the smooth-seeded (Lupinus 
      angustifolius L., Lupinus cryptanthus Shuttlew., Lupinus micranthus Guss.) and 
      rough-seeded (Lupinus cosentinii Guss. and Lupinus pilosus Murr.) lupin species. 
      Comparative cytogenetic mapping was done using FISH with oligonucleotide probes 
      and previously published chromosome-specific bacterial artificial chromosome 
      (BAC) clones. Oligonucleotide probes were designed to cover both arms of 
      chromosome Lang06 of the L. angustifolius reference genome separately. The 
      chromosome was chosen for the in-depth study due to observed structural 
      variability among wild lupin species revealed by BAC-FISH and supplemented by in 
      silico mapping of recently released lupin genome assemblies. The results 
      highlighted changes in synteny within the Lang06 region between the lupin 
      species, including putative translocations, inversions, and/or non-allelic 
      homologous recombination, which would have accompanied the evolution and 
      speciation.
FAU - Bielski, Wojciech
AU  - Bielski W
AUID- ORCID: 0000-0002-6174-2898
AD  - Department of Genomics, Institute of Plant Genetics, Polish Academy of Sciences, 
      60-479 Poznan, Poland.
FAU - Ksiazkiewicz, Michal
AU  - Ksiazkiewicz M
AUID- ORCID: 0000-0002-3382-8504
AD  - Department of Genomics, Institute of Plant Genetics, Polish Academy of Sciences, 
      60-479 Poznan, Poland.
FAU - Simonikova, Denisa
AU  - Simonikova D
AUID- ORCID: 0000-0002-1066-0577
AD  - Institute of Experimental Botany of the Czech Academy of Sciences, Centre of the 
      Region Hana for Biotechnological and Agricultural Research, 77900 Olomouc, Czech 
      Republic.
FAU - Hribova, Eva
AU  - Hribova E
AUID- ORCID: 0000-0002-6868-4344
AD  - Institute of Experimental Botany of the Czech Academy of Sciences, Centre of the 
      Region Hana for Biotechnological and Agricultural Research, 77900 Olomouc, Czech 
      Republic.
FAU - Susek, Karolina
AU  - Susek K
AUID- ORCID: 0000-0002-8518-4720
AD  - Department of Genomics, Institute of Plant Genetics, Polish Academy of Sciences, 
      60-479 Poznan, Poland.
FAU - Naganowska, Barbara
AU  - Naganowska B
AUID- ORCID: 0000-0002-4393-2655
AD  - Department of Genomics, Institute of Plant Genetics, Polish Academy of Sciences, 
      60-479 Poznan, Poland.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201210
PL  - Switzerland
TA  - Genes (Basel)
JT  - Genes
JID - 101551097
SB  - IM
MH  - *Chromosome Mapping
MH  - Chromosomes, Artificial, Bacterial
MH  - Chromosomes, Plant/*genetics
MH  - *Genome, Plant
MH  - In Situ Hybridization, Fluorescence
MH  - Lupinus/*genetics
PMC - PMC7764521
OTO - NOTNLM
OT  - FISH
OT  - chromosome evolution
OT  - comparative-mapping
OT  - cytogenetics
OT  - karyotype evolution
OT  - lupin
OT  - oligo-painting
OT  - oligonucleotide probes
OT  - wild species
COIS- The authors declare no conflict of interest. The funders had no role in the 
      design of the study; in the collection, analyses, or interpretation of data; in 
      the writing of the manuscript, or in the decision to publish the results.
EDAT- 2020/12/17 06:00
MHDA- 2021/07/23 06:00
PMCR- 2020/12/10
CRDT- 2020/12/16 01:02
PHST- 2020/11/06 00:00 [received]
PHST- 2020/12/07 00:00 [revised]
PHST- 2020/12/08 00:00 [accepted]
PHST- 2020/12/16 01:02 [entrez]
PHST- 2020/12/17 06:00 [pubmed]
PHST- 2021/07/23 06:00 [medline]
PHST- 2020/12/10 00:00 [pmc-release]
AID - genes11121489 [pii]
AID - genes-11-01489 [pii]
AID - 10.3390/genes11121489 [doi]
PST - epublish
SO  - Genes (Basel). 2020 Dec 10;11(12):1489. doi: 10.3390/genes11121489.