PMID- 33322269
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201230
IS  - 2309-608X (Electronic)
IS  - 2309-608X (Linking)
VI  - 6
IP  - 4
DP  - 2020 Dec 11
TI  - Immunoproteomic Analysis Reveals Novel Candidate Antigens for the Diagnosis of 
      Paracoccidioidomycosis Due to Paracoccidioides lutzii.
LID - 10.3390/jof6040357 [doi]
LID - 357
AB  - Paracoccidioidomycosis (PCM) is a life-threatening systemic infection caused by 
      the fungal pathogen Paracoccidioides brasiliensis and related species. 
      Whole-genome sequencing and stage-specific proteomic analysis of Paracoccidioides 
      offer the opportunity to profile humoral immune responses against P. lutzii and 
      P. brasiliensis s. str. infection using innovative screening approaches. Here, an 
      immunoproteomic approach was used to identify PCM-associated antigens that elicit 
      immune responses by combining 2-D electrophoresis of P. lutzii and P. 
      brasiliensis proteomes, immunological detection using a gold-standard serum, and 
      mass spectrometry analysis. A total of 16 and 25 highly immunoreactive proteins 
      were identified in P. lutzii and P. brasiliensis, respectively, and 29 were shown 
      to be the novel antigens for Paracoccidioides species, including seven 
      uncharacterized proteins. Among the panel of proteins identified, most are 
      involved in metabolic pathways, carbon metabolism, and biosynthesis of secondary 
      metabolites in both immunoproteomes. Remarkably, six isoforms of the 
      surface-associated enolase in the range of 54 kDa were identified as the major 
      antigens in human PCM due to P. lutzii. These novel immunoproteomes of 
      Paracoccidioides will be employed to develop a sensitive and affordable 
      point-of-care diagnostic assay and an effective vaccine to identify infected 
      hosts and prevent infection and development of human PCM. These findings provide 
      a unique opportunity for the refinement of diagnostic tools of this important 
      neglected systemic mycosis, which is usually associated with poverty.
FAU - Rodrigues, Anderson Messias
AU  - Rodrigues AM
AUID- ORCID: 0000-0003-0891-4563
AD  - Laboratory of Emerging Fungal Pathogens, Department of Microbiology, Immunology, 
      and Parasitology, Discipline of Cellular Biology, Federal University of Sao Paulo 
      (UNIFESP), Sao Paulo 04023062, Brazil.
FAU - Kubitschek-Barreira, Paula Helena
AU  - Kubitschek-Barreira PH
AD  - Department of Cellular Biology, Roberto Alcantara Gomes Institute of Biology, Rio 
      de Janeiro State University (UERJ), Rio de Janeiro 20511010, Brazil.
FAU - Pinheiro, Breno Goncalves
AU  - Pinheiro BG
AUID- ORCID: 0000-0001-9134-0657
AD  - Laboratory of Emerging Fungal Pathogens, Department of Microbiology, Immunology, 
      and Parasitology, Discipline of Cellular Biology, Federal University of Sao Paulo 
      (UNIFESP), Sao Paulo 04023062, Brazil.
FAU - Teixeira-Ferreira, Andre
AU  - Teixeira-Ferreira A
AUID- ORCID: 0000-0001-6690-6620
AD  - Toxinology Laboratory, Department of Physiology and Pharmacodynamics, Fiocruz, 
      Rio de Janeiro 21040900, Brazil.
FAU - Hahn, Rosane Christine
AU  - Hahn RC
AD  - Laboratory of Mycology/Research, Faculty of Medicine, Federal University of Mato 
      Grosso, Cuiaba 78060900, Brazil.
AD  - Julio Muller University Hospital, Federal University of Mato Grosso, Cuiaba 
      78048902, Brazil.
FAU - de Camargo, Zoilo Pires
AU  - de Camargo ZP
AD  - Laboratory of Emerging Fungal Pathogens, Department of Microbiology, Immunology, 
      and Parasitology, Discipline of Cellular Biology, Federal University of Sao Paulo 
      (UNIFESP), Sao Paulo 04023062, Brazil.
AD  - Department of Medicine, Discipline of Infectious Diseases, Federal University of 
      Sao Paulo (UNIFESP), Sao Paulo 04023062, Brazil.
LA  - eng
GR  - 2017/27265-5/Fundacao de Amparo a Pesquisa do Estado de Sao Paulo/
GR  - 2018/21460-3/Fundacao de Amparo a Pesquisa do Estado de Sao Paulo/
GR  - 429594/2018-6/Conselho Nacional de Desenvolvimento Cientifico e Tecnologico/
GR  - 88887.177846/2018-00/Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior/
PT  - Journal Article
DEP - 20201211
PL  - Switzerland
TA  - J Fungi (Basel)
JT  - Journal of fungi (Basel, Switzerland)
JID - 101671827
PMC - PMC7770604
OTO - NOTNLM
OT  - Paracoccidioides brasiliensis
OT  - Paracoccidioides lutzii
OT  - biomarker
OT  - diagnosis
OT  - endemic mycosis
OT  - immunoproteomic
OT  - paracoccidioidomycosis
OT  - proteomic
OT  - serology
OT  - systemic mycosis
COIS- The authors declare no conflict of interest. The funders had no role in the 
      design of the study; the collection, analyses or interpretation of data; the 
      writing of the manuscript; or the decision to publish the results.
EDAT- 2020/12/17 06:00
MHDA- 2020/12/17 06:01
PMCR- 2020/12/11
CRDT- 2020/12/16 01:03
PHST- 2020/11/16 00:00 [received]
PHST- 2020/12/04 00:00 [revised]
PHST- 2020/12/08 00:00 [accepted]
PHST- 2020/12/16 01:03 [entrez]
PHST- 2020/12/17 06:00 [pubmed]
PHST- 2020/12/17 06:01 [medline]
PHST- 2020/12/11 00:00 [pmc-release]
AID - jof6040357 [pii]
AID - jof-06-00357 [pii]
AID - 10.3390/jof6040357 [doi]
PST - epublish
SO  - J Fungi (Basel). 2020 Dec 11;6(4):357. doi: 10.3390/jof6040357.