PMID- 33322310
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201227
IS  - 2075-4418 (Print)
IS  - 2075-4418 (Electronic)
IS  - 2075-4418 (Linking)
VI  - 10
IP  - 12
DP  - 2020 Dec 11
TI  - The Influence of Calcification Factors and Endothelial-Dysfunction Factors on the 
      Development of Unstable Atherosclerotic Plaques.
LID - 10.3390/diagnostics10121074 [doi]
LID - 1074
AB  - BACKGROUND: This study aimed to evaluate changes in markers of calcification and 
      of endothelial dysfunction during the development of calcification and 
      instability of atherosclerotic plaques and to identify associations of 
      calcification factors with the formation of unstable plaques. METHODS: We 
      analyzed 44 male patients with coronary atherosclerosis who underwent 
      endarterectomy in coronary arteries during coronary bypass surgery. The 
      endarterectomy material (intima/media) was examined using histological and 
      biochemical methods, and the stability and calcification degree of 
      atherosclerotic plaques were assessed. In homogenates of the tissue samples and 
      in blood, concentrations of osteoprotegerin, osteocalcin, osteopontin, 
      osteonectin, monocyte-chemoattractant protein type 1 (MCP-1), soluble vascular 
      cell adhesion molecule 1 (sVCAM-1), and E-selectin were determined by enzyme 
      immunoassays. RESULTS: Unstable atherosclerotic plaques proved to be calcified 
      more frequently (80.4% of plaques) than stable ones (45.0%). Osteonectin, 
      E-selectin, and sVCAM-1 levels were lower in unstable plaques and plaques with 
      large calcification deposits. Osteocalcin content increased with the increasing 
      size of the calcification deposits in plaque. Blood osteocalcin concentration 
      directly correlated with osteocalcin concentration in atherosclerotic plaques and 
      was higher in the blood of patients with calcified plaques in coronary arteries. 
      CONCLUSIONS: The results provide the basis for further research on the 
      suitability of osteocalcin as a potential biomarker of an unstable calcified 
      atherosclerotic plaque in a coronary artery.
FAU - Polonskaya, Yana V
AU  - Polonskaya YV
AUID- ORCID: 0000-0002-3538-0280
AD  - Research Institute of Internal and Preventive Medicine, Branch of the Institute 
      of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 
      Bogatkova Str. 175/1, 630089 Novosibirsk, Russia.
FAU - Kashtanova, Elena V
AU  - Kashtanova EV
AD  - Research Institute of Internal and Preventive Medicine, Branch of the Institute 
      of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 
      Bogatkova Str. 175/1, 630089 Novosibirsk, Russia.
FAU - Murashov, Ivan S
AU  - Murashov IS
AUID- ORCID: 0000-0002-3712-1258
AD  - The Federal State Budgetary Institution "National Medical Research Center named 
      Academician E.N. Meshalkin" of the Ministry of Health of the Russian Federation, 
      630055 Novosibirsk, Russia.
FAU - Kurguzov, Aleksei V
AU  - Kurguzov AV
AD  - The Federal State Budgetary Institution "National Medical Research Center named 
      Academician E.N. Meshalkin" of the Ministry of Health of the Russian Federation, 
      630055 Novosibirsk, Russia.
FAU - Sadovski, Evgeny V
AU  - Sadovski EV
AD  - Research Institute of Internal and Preventive Medicine, Branch of the Institute 
      of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 
      Bogatkova Str. 175/1, 630089 Novosibirsk, Russia.
FAU - Maslatsov, Nikolay A
AU  - Maslatsov NA
AD  - Research Institute of Internal and Preventive Medicine, Branch of the Institute 
      of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 
      Bogatkova Str. 175/1, 630089 Novosibirsk, Russia.
FAU - Stakhneva, Ekaterina M
AU  - Stakhneva EM
AUID- ORCID: 0000-0003-0484-6540
AD  - Research Institute of Internal and Preventive Medicine, Branch of the Institute 
      of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 
      Bogatkova Str. 175/1, 630089 Novosibirsk, Russia.
FAU - Chernyavskii, Alexander M
AU  - Chernyavskii AM
AD  - The Federal State Budgetary Institution "National Medical Research Center named 
      Academician E.N. Meshalkin" of the Ministry of Health of the Russian Federation, 
      630055 Novosibirsk, Russia.
FAU - Ragino, Yuliya I
AU  - Ragino YI
AUID- ORCID: 0000-0002-4936-8362
AD  - Research Institute of Internal and Preventive Medicine, Branch of the Institute 
      of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 
      Bogatkova Str. 175/1, 630089 Novosibirsk, Russia.
LA  - eng
GR  - 19-015-00055small a, Cyrillic/Russian Foundation for Basic Research/
GR  - capital A, Cyrilliccapital A, Cyrilliccapital A, Cyrilliccapital A, Cyrillic-capital A, Cyrillic17-117112850280-2/Russian Academy of Sciences/
GR  - 0324-2017-0048/Russian Academy of Sciences/
PT  - Journal Article
DEP - 20201211
PL  - Switzerland
TA  - Diagnostics (Basel)
JT  - Diagnostics (Basel, Switzerland)
JID - 101658402
PMC - PMC7764445
OTO - NOTNLM
OT  - E-selectin
OT  - calcification of atherosclerotic plaques
OT  - osteocalcin
OT  - osteonectin
OT  - osteopontin
OT  - sVCAM
OT  - unstable atherosclerotic plaque
COIS- The authors declare no conflict of interest.
EDAT- 2020/12/17 06:00
MHDA- 2020/12/17 06:01
PMCR- 2020/12/11
CRDT- 2020/12/16 01:03
PHST- 2020/11/30 00:00 [received]
PHST- 2020/12/08 00:00 [revised]
PHST- 2020/12/09 00:00 [accepted]
PHST- 2020/12/16 01:03 [entrez]
PHST- 2020/12/17 06:00 [pubmed]
PHST- 2020/12/17 06:01 [medline]
PHST- 2020/12/11 00:00 [pmc-release]
AID - diagnostics10121074 [pii]
AID - diagnostics-10-01074 [pii]
AID - 10.3390/diagnostics10121074 [doi]
PST - epublish
SO  - Diagnostics (Basel). 2020 Dec 11;10(12):1074. doi: 10.3390/diagnostics10121074.