PMID- 33322648
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201227
IS  - 2227-9059 (Print)
IS  - 2227-9059 (Electronic)
IS  - 2227-9059 (Linking)
VI  - 8
IP  - 12
DP  - 2020 Dec 11
TI  - Differential Role of Circulating microRNAs to Track Progression and 
      Pre-Symptomatic Stage of Chronic Heart Failure: A Pilot Study.
LID - 10.3390/biomedicines8120597 [doi]
LID - 597
AB  - (1)Background: Chronic heart failure (CHF) contributes to the overall burden of 
      cardiovascular disease. Early identification of at-risk individuals may 
      facilitate the targeting of precision therapies. Plasma microRNAs are promising 
      circulating biomarkers for their implications with cardiac pathologies. In this 
      pilot study, we investigate the possible exploitability of circulating micro-RNAs 
      (miRNAs) to track chronic heart failure (CHF) occurrence, and progression from 
      NYHA class I to IV. (2)Methods: We screened 367 microRNAs using TaqMan microRNA 
      Arrays in plasma samples from healthy controls (HC) and CHF NYHA-class I-to-IV 
      patients (5/group). Validation was performed by singleplex assays on 10 HC and 61 
      CHF subjects. Differences in the expression of validated microRNAs were evaluated 
      through analysis of covariance (ANCOVA). Associations between N-terminal pro-BNP 
      (NT-proBNP), left ventricular end-diastolic volume (LVEDV) or peak oxygen uptake 
      (VO2 peak) and plasma microRNA were assessed by multivariable linear regression 
      analysis. (3)Results: Twelve microRNAs showed higher expression in CHF patients 
      vs. HC. Seven microRNAs were associated with NT-proBNP concentration; of these, 
      miR-423-5p was also an independent predictor of LVEDV. Moreover, miR-499-5p was a 
      predictor of the VO2 peak. Finally, a cluster of 5 miRNAs discriminated New York 
      Heart Association (NYHA) class-I from HC subjects. (4)Conclusions: Our data 
      suggest that circulating miRNAs have the potential to serve as 
      pathophysiology-based markers of HF status and progression, and as indicators of 
      pre-symptomatic individuals.
FAU - D'Alessandra, Yuri
AU  - D'Alessandra Y
AUID- ORCID: 0000-0002-3440-2353
AD  - Immunology and Functional Genomics Unit, Centro Cardiologico Monzino-IRCCS, 20138 
      Milan, Italy.
FAU - Chiesa, Mattia
AU  - Chiesa M
AUID- ORCID: 0000-0001-7427-9954
AD  - Immunology and Functional Genomics Unit, Centro Cardiologico Monzino-IRCCS, 20138 
      Milan, Italy.
FAU - Carena, Maria Cristina
AU  - Carena MC
AD  - Vascular Biology and Regenerative Medicine Unit, Centro Cardiologico 
      Monzino-IRCCS, 20138 Milan, Italy.
FAU - Beltrami, Antonio Paolo
AU  - Beltrami AP
AUID- ORCID: 0000-0002-0679-2710
AD  - Department of Medicine (DAME), University of Udine, 33100 Udine, Italy.
FAU - Rizzo, Paola
AU  - Rizzo P
AUID- ORCID: 0000-0001-7174-9674
AD  - Maria Cecilia Hospital, GVM Care & Research, 48033 Cotignola, Italy.
AD  - Department of Morphology, Surgery and Experimental Medicine and Laboratory for 
      Technologies of Advanced Therapies (LTTA), University of Ferrara, 44121 Ferrara, 
      Italy.
FAU - Buzzetti, Marta
AU  - Buzzetti M
AUID- ORCID: 0000-0003-3415-0982
AD  - Immunology and Functional Genomics Unit, Centro Cardiologico Monzino-IRCCS, 20138 
      Milan, Italy.
FAU - Ricci, Veronica
AU  - Ricci V
AD  - Immunology and Functional Genomics Unit, Centro Cardiologico Monzino-IRCCS, 20138 
      Milan, Italy.
AD  - Dipartimento di Medicina Clinica e Chirurgia, Universita deli Studi di Napoli 
      Federico II, 80131 Napoli, Italy.
FAU - Ferrari, Roberto
AU  - Ferrari R
AD  - Maria Cecilia Hospital, GVM Care & Research, 48033 Cotignola, Italy.
AD  - Centro Cardiologico Universitario di Ferrara, University of Ferrara, 44124 
      Ferrara, Italy.
FAU - Fucili, Alessandro
AU  - Fucili A
AUID- ORCID: 0000-0002-1843-2923
AD  - Centro Cardiologico Universitario di Ferrara, University of Ferrara, 44124 
      Ferrara, Italy.
FAU - Livi, Ugolino
AU  - Livi U
AD  - Department of Medicine (DAME), University of Udine, 33100 Udine, Italy.
FAU - Aleksova, Aneta
AU  - Aleksova A
AUID- ORCID: 0000-0001-7711-9905
AD  - Cardiovascular Department, Azienda Sanitaria Universitaria Giuliano Isontina 
      (ASUGI), University of Trieste, 34148 Trieste, Italy.
FAU - Pompilio, Giulio
AU  - Pompilio G
AD  - Vascular Biology and Regenerative Medicine Unit, Centro Cardiologico 
      Monzino-IRCCS, 20138 Milan, Italy.
AD  - Dipartimento di Scienze Cliniche e di Comunita, Universita degli Studi di Milano, 
      20122 Milan, Italy.
FAU - Colombo, Gualtiero I
AU  - Colombo GI
AUID- ORCID: 0000-0002-7348-1939
AD  - Immunology and Functional Genomics Unit, Centro Cardiologico Monzino-IRCCS, 20138 
      Milan, Italy.
LA  - eng
GR  - RC-2010-BIO 64-ID-1489855/Ministero della Salute/
GR  - 5 per thousand 2015/Ministero della Salute/
PT  - Journal Article
DEP - 20201211
PL  - Switzerland
TA  - Biomedicines
JT  - Biomedicines
JID - 101691304
PMC - PMC7764340
OTO - NOTNLM
OT  - biomarkers
OT  - heart failure
OT  - microRNA
COIS- The authors declare no conflict of interest.
EDAT- 2020/12/17 06:00
MHDA- 2020/12/17 06:01
PMCR- 2020/12/11
CRDT- 2020/12/16 01:04
PHST- 2020/11/11 00:00 [received]
PHST- 2020/12/07 00:00 [revised]
PHST- 2020/12/08 00:00 [accepted]
PHST- 2020/12/16 01:04 [entrez]
PHST- 2020/12/17 06:00 [pubmed]
PHST- 2020/12/17 06:01 [medline]
PHST- 2020/12/11 00:00 [pmc-release]
AID - biomedicines8120597 [pii]
AID - biomedicines-08-00597 [pii]
AID - 10.3390/biomedicines8120597 [doi]
PST - epublish
SO  - Biomedicines. 2020 Dec 11;8(12):597. doi: 10.3390/biomedicines8120597.