PMID- 33323546
OWN - NLM
STAT- MEDLINE
DCOM- 20210903
LR  - 20210903
IS  - 1945-4589 (Electronic)
IS  - 1945-4589 (Linking)
VI  - 13
IP  - 2
DP  - 2020 Dec 11
TI  - Antitumor effect of poly lactic acid nanoparticles loaded with cisplatin and 
      chloroquine on the oral squamous cell carcinoma.
PG  - 2593-2603
LID - 10.18632/aging.202297 [doi]
AB  - PURPOSE: Poly lactic acid (PLA) combined with cisplatin-chloroquine nanoparticles 
      (CDDP/CQ-PLA NPs) and PLA combined with cisplatin nanoparticles (CDDP-PLA NPs) 
      were prepared to investigate their inhibitory effects on the proliferation of 
      oral squamous cell carcinoma (OSCC) Cal-27cell line. PATIENTS AND METHODS: We 
      prepared CDDP/CQ-PLA NPs and CDDP-PLA NPs. Transmission electron microscopy (TEM) 
      and dynamic light scattering (DLS) were used to detect the physiological 
      characteristics and particle size parameters of drug-loaded nanoparticles. The 
      drug concentration and cumulative release were measured by UV and visible 
      spectrophotometer. MTT assay was used to detect viability of Cal-27 cells. 
      Annexin/PI staining was used to detect cell apoptosis. Biological kits were used 
      to detect malondialdehyde (MDA) content, catalase (CAT) activity, antioxidant 
      enzyme superoxide dismutase (SOD) activity and glutathione peroxidase (GSH PX) 
      activity in Cal-27 cells. Western blot was used to detect apoptosis and autophagy 
      of Cal-27 cells. RESULTS: CDDP/CQ-PLA NPs and CDDP -PLA NPs had good drug loaded 
      nanoparticles and drug release. CDDP/CQ-PLA NPs showed higher ROS and apoptosis 
      rate, and lower autophagy than CDDP-PLA NPs. CONCLUSION: CDDP/CQ-PLA NPs reduced 
      autophagy and enhanced ROS and apoptosis of Cal-27 cells, which shows a potential 
      in the clinical treatment of OSCC.
FAU - Li, Qiang
AU  - Li Q
AD  - Department of Oral and Maxillofacial Surgery, Cangzhou Central Hospital, 
      Cangzhou, Hebei Province, China.
FAU - Liu, Xia
AU  - Liu X
AD  - Department of Basic Nursing, Cangzhou Medical College, Cangzhou, Hebei Province, 
      China.
FAU - Yan, Wei
AU  - Yan W
AD  - Department of Oral and Maxillofacial Surgery, Cangzhou Central Hospital, 
      Cangzhou, Hebei Province, China.
FAU - Chen, Yong
AU  - Chen Y
AD  - Department of Oral and Maxillofacial Surgery, Cangzhou Central Hospital, 
      Cangzhou, Hebei Province, China.
LA  - eng
PT  - Journal Article
DEP - 20201211
PL  - United States
TA  - Aging (Albany NY)
JT  - Aging
JID - 101508617
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Drug Carriers)
RN  - 0 (Polyesters)
RN  - 459TN2L5F5 (poly(lactide))
RN  - 886U3H6UFF (Chloroquine)
RN  - Q20Q21Q62J (Cisplatin)
SB  - IM
MH  - Antineoplastic Agents/administration & dosage/*therapeutic use
MH  - Apoptosis/drug effects
MH  - Autophagy/drug effects
MH  - Carcinoma, Squamous Cell/*drug therapy
MH  - Cell Line, Tumor
MH  - Cell Proliferation/drug effects
MH  - Chloroquine/administration & dosage/*therapeutic use
MH  - Cisplatin/administration & dosage/*therapeutic use
MH  - Drug Carriers
MH  - Drug Delivery Systems
MH  - Drug Liberation
MH  - Humans
MH  - Mouth Neoplasms/*drug therapy
MH  - Nanoparticles
MH  - Oxidative Stress
MH  - Particle Size
MH  - Polyesters/administration & dosage/*therapeutic use
PMC - PMC7880364
OTO - NOTNLM
OT  - dynamic light scattering
OT  - oral squamous cell carcinoma
OT  - poly lactic acid
OT  - transmission electron microscopy
COIS- CONFLICTS OF INTEREST: The authors declare that they have no conflicts of 
      interest.
EDAT- 2020/12/17 06:00
MHDA- 2021/09/04 06:00
PMCR- 2021/01/31
CRDT- 2020/12/16 05:29
PHST- 2020/02/08 00:00 [received]
PHST- 2020/04/17 00:00 [accepted]
PHST- 2020/12/17 06:00 [pubmed]
PHST- 2021/09/04 06:00 [medline]
PHST- 2020/12/16 05:29 [entrez]
PHST- 2021/01/31 00:00 [pmc-release]
AID - 202297 [pii]
AID - 10.18632/aging.202297 [doi]
PST - ppublish
SO  - Aging (Albany NY). 2020 Dec 11;13(2):2593-2603. doi: 10.18632/aging.202297. Epub 
      2020 Dec 11.