PMID- 33324039
OWN - NLM
STAT- MEDLINE
DCOM- 20210921
LR  - 20220419
IS  - 1177-8881 (Electronic)
IS  - 1177-8881 (Linking)
VI  - 14
DP  - 2020
TI  - Neohesperidin Ameliorates Steroid-Induced Osteonecrosis of the Femoral Head by 
      Inhibiting the Histone Modification of lncRNA HOTAIR.
PG  - 5419-5430
LID - 10.2147/DDDT.S255276 [doi]
AB  - BACKGROUND: Neohesperidin (NH) and lncRNA HOTAIR (HOTAIR) could regulate 
      osteoclastic and osteogenic differentiation. This study aimed to explore whether 
      HOTAIR-mediated osteogenic differentiation was regulated by NH. METHODS: 
      Steroid-induced osteonecrosis of the femoral head (SONFH) mice model was 
      established. Histopathological changes in mouse osteonecrosis tissues were 
      detected by hematoxylin-eosin staining. Bone marrow stromal cells (BMSCs) were 
      isolated from healthy mice bone marrow samples by Ficoll density gradient and 
      identified by flow cytometry. After treating the BMSCs with NH and dexamethasone 
      or transfecting with HOTAIR overexpression plasmids and siHOTAIR, histone 
      modification of HOTAIR, the cell viability, osteogenic differentiation, and 
      adipogenic differentiation were detected by chromatin immunoprecipitation, MTT, 
      Alizarin Red and Oil Red O staining, respectively. The expressions of HOTAIR and 
      differentiation-related factors in the BMSCs were detected by RT-qPCR and Western 
      blot. RESULTS: HOTAIR was highly expressed in SONFH model mice. NH ameliorated 
      histopathological changes in the model mice, but the effect was reversed by 
      overexpressed HOTAIR. NH increased viability of BMSCs and the H3K27me3 occupancy 
      of HOTAIR, but decreased the expression and the H3K4me3 occupancy of HOTAIR. 
      HOTAIR expression was down-regulated in BMSCs after osteogenic differentiation 
      but was up-regulated after adipogenic differentiation. HOTAIR overexpression 
      inhibited osteogenic differentiation and the expressions of RUNX2, OCN, and ALP, 
      but increased adipogenic differentiation and the expressions of LPL and PPARr in 
      BMSCs; moreover, the opposite results were observed in siHOTAIR. CONCLUSION: NH 
      ameliorated SONFH by inhibiting the histone modifications of HOTAIR.
CI  - (c) 2020 Yuan et al.
FAU - Yuan, Shuai
AU  - Yuan S
AD  - Department of Joint Surgery and Sports Medicine, Changzheng Hospital, Naval 
      Medical University, Shanghai, People's Republic of China.
FAU - Zhang, Chuanxin
AU  - Zhang C
AD  - Department of Joint Surgery and Sports Medicine, Changzheng Hospital, Naval 
      Medical University, Shanghai, People's Republic of China.
FAU - Zhu, Yunli
AU  - Zhu Y
AD  - Department of Joint Surgery and Sports Medicine, Changzheng Hospital, Naval 
      Medical University, Shanghai, People's Republic of China.
FAU - Wang, Bo
AU  - Wang B
AD  - Department of Joint Surgery and Sports Medicine, Changzheng Hospital, Naval 
      Medical University, Shanghai, People's Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20201207
PL  - New Zealand
TA  - Drug Des Devel Ther
JT  - Drug design, development and therapy
JID - 101475745
RN  - 0 (HOTAIR long non-coding RNA, mouse)
RN  - 0 (Histones)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (Steroids)
RN  - E750O06Y6O (Hesperidin)
RN  - OA5C88H3L0 (neohesperidin)
SB  - IM
MH  - Animals
MH  - Femur Head/*drug effects/metabolism/pathology
MH  - Hesperidin/*analogs & derivatives/pharmacology
MH  - Histones/*antagonists & inhibitors/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Osteogenesis/*drug effects
MH  - RNA, Long Noncoding/genetics/*metabolism
MH  - Steroids/antagonists & inhibitors/pharmacology
PMC - PMC7733036
OTO - NOTNLM
OT  - HOTAIR
OT  - adipogenic
OT  - bone marrow stromal cells
OT  - neohesperidin
OT  - osteogenesis
COIS- Non-financial competing interests. The authors report no other potential 
      conflicts of interest for this work.
EDAT- 2020/12/17 06:00
MHDA- 2021/09/22 06:00
PMCR- 2020/12/07
CRDT- 2020/12/16 05:35
PHST- 2020/03/24 00:00 [received]
PHST- 2020/10/08 00:00 [accepted]
PHST- 2020/12/16 05:35 [entrez]
PHST- 2020/12/17 06:00 [pubmed]
PHST- 2021/09/22 06:00 [medline]
PHST- 2020/12/07 00:00 [pmc-release]
AID - 255276 [pii]
AID - 10.2147/DDDT.S255276 [doi]
PST - epublish
SO  - Drug Des Devel Ther. 2020 Dec 7;14:5419-5430. doi: 10.2147/DDDT.S255276. 
      eCollection 2020.