PMID- 33326091
OWN - NLM
STAT- MEDLINE
DCOM- 20210401
LR  - 20210401
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2226
DP  - 2021
TI  - Tissue Preservation and FFPE Samples: Optimized Nucleic Acids Isolation in Ewing 
      Sarcoma.
PG  - 27-38
LID - 10.1007/978-1-0716-1020-6_3 [doi]
AB  - Different methods have been described for the preservation of biopsy or resection 
      samples. In the routine pathology, the cheapest and most commonly used is 
      fixation of samples in formalin and embedding in paraffin (FFPE samples). This 
      method preserves tissue samples for a very long time and is suitable for several 
      specialized techniques such as fluorescence in situ hybridization (FISH) and 
      immunohistochemistry, the latter being the most frequent and often the only 
      additional method used for establishment of final diagnosis. However, in light of 
      the growing need of next-generation sequencing and microarray technologies that 
      are often very helpful to establish and/or confirm diagnoses in the field of 
      pediatric sarcoma (including Ewing sarcoma), preservation of high-quality and 
      quantity of nucleic acids (DNA/RNA) is desirable. Herein, we describe how to 
      ideally preserve samples, as well as how to proceed to isolate nucleic acids for 
      successful subsequent molecular assays with a special focus on Ewing sarcoma 
      samples.
FAU - Romero-Perez, Laura
AU  - Romero-Perez L
AD  - Division of Translational Pediatric Sarcoma Research, German Cancer Research 
      Center (DKFZ), Heidelberg, Germany. Laura.romeroperez@dkfz-heidelberg.de.
AD  - Hopp Children's Cancer Center (KiTZ), Heidelberg, Germany. 
      Laura.romeroperez@dkfz-heidelberg.de.
AD  - German Cancer Consortium (DKTK), Heidelberg, Germany. 
      Laura.romeroperez@dkfz-heidelberg.de.
FAU - Grunewald, Thomas G P
AU  - Grunewald TGP
AD  - Division of Translational Pediatric Sarcoma Research, German Cancer Research 
      Center (DKFZ), Hopp Children's Cancer Center (KiTZ), Heidelberg, Germany.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Nucleic Acids)
SB  - IM
MH  - Biopsy
MH  - Cryopreservation
MH  - Humans
MH  - *Immunohistochemistry/methods
MH  - *Molecular Diagnostic Techniques/methods
MH  - Nucleic Acids/isolation & purification
MH  - Paraffin Embedding
MH  - Preservation, Biological
MH  - Sarcoma, Ewing/*diagnosis/pathology
MH  - Tissue Fixation
MH  - *Tissue Preservation/methods
OTO - NOTNLM
OT  - DNA
OT  - Ewing sarcoma
OT  - FFPE samples
OT  - Molecular assays
OT  - Preservation
OT  - RNA
EDAT- 2020/12/17 06:00
MHDA- 2021/04/02 06:00
CRDT- 2020/12/16 12:13
PHST- 2020/12/16 12:13 [entrez]
PHST- 2020/12/17 06:00 [pubmed]
PHST- 2021/04/02 06:00 [medline]
AID - 10.1007/978-1-0716-1020-6_3 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2021;2226:27-38. doi: 10.1007/978-1-0716-1020-6_3.