PMID- 33326094
OWN - NLM
STAT- MEDLINE
DCOM- 20210401
LR  - 20210401
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2226
DP  - 2021
TI  - Molecular Approaches to Diagnosis in Ewing Sarcoma: Fluorescence In Situ 
      Hybridization (FISH).
PG  - 65-83
LID - 10.1007/978-1-0716-1020-6_6 [doi]
AB  - The differential diagnosis of small round cell tumors (SRCT) crucially relies on 
      the synoptic evaluation of morphology, immunohistochemical patterns, and 
      molecular features. Though the implementation of broad RNA sequencing in 
      diagnostic molecular pathology routines has substantially changed the standards 
      of molecular affirmation of diagnoses, fluorescence in situ hybridization (FISH) 
      on formalin-fixed, paraffin-embedded (FFPE) tissue sections is still an 
      elementary tool to provide a rapid molecular corroboration of diagnoses, 
      essentially required for therapeutic decisions. We discuss here the major FISH 
      approaches currently employed in diagnostic molecular pathology, addressing 
      classic Ewing sarcoma and differential diagnoses among SRCT which cannot 
      sufficiently be ruled out by immunohistochemistry. This chapter will approach 
      technical issues but particularly strategies and pitfalls in the interpretation 
      of FISH patterns.
FAU - Trautmann, Marcel
AU  - Trautmann M
AD  - Division of Translational Pathology, Gerhard-Domagk-Institute of Pathology, 
      Munster, Germany.
FAU - Hartmann, Wolfgang
AU  - Hartmann W
AD  - Division of Translational Pathology, Gerhard-Domagk-Institute of Pathology, 
      Munster, Germany. wolfgang.hartmann@ukmuenster.de.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Biomarkers, Tumor)
SB  - IM
MH  - *Biomarkers, Tumor
MH  - Bone Neoplasms/*diagnosis/etiology
MH  - Chromosome Aberrations
MH  - Diagnosis, Differential
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Microscopy, Fluorescence
MH  - *Molecular Diagnostic Techniques
MH  - Sarcoma, Ewing/*diagnosis/etiology
OTO - NOTNLM
OT  - BCOR
OT  - CIC
OT  - EWSR1
OT  - Ewing sarcoma
OT  - FISH
OT  - FUS
OT  - Small round cell tumor
EDAT- 2020/12/17 06:00
MHDA- 2021/04/02 06:00
CRDT- 2020/12/16 12:13
PHST- 2020/12/16 12:13 [entrez]
PHST- 2020/12/17 06:00 [pubmed]
PHST- 2021/04/02 06:00 [medline]
AID - 10.1007/978-1-0716-1020-6_6 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2021;2226:65-83. doi: 10.1007/978-1-0716-1020-6_6.